Experiment: L-Leucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_1595 and Shewana3_1596 are separated by 231 nucleotides Shewana3_1596 and Shewana3_1597 are separated by 58 nucleotides Shewana3_1597 and Shewana3_1598 are separated by 10 nucleotides
Shewana3_1595: Shewana3_1595 - PKD domain-containing protein (RefSeq), at 1,871,397 to 1,874,243
_1595
Shewana3_1596: Shewana3_1596 - hypothetical protein (RefSeq), at 1,874,475 to 1,874,903
_1596
Shewana3_1597: Shewana3_1597 - hypothetical protein (RefSeq), at 1,874,962 to 1,875,858
_1597
Shewana3_1598: Shewana3_1598 - hypothetical protein (RefSeq), at 1,875,869 to 1,876,627
_1598
Position (kb) 1874
1875 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1873.537 kb on + strand, within Shewana3_1595 at 1873.545 kb on + strand, within Shewana3_1595 at 1873.580 kb on + strand, within Shewana3_1595 at 1873.588 kb on - strand, within Shewana3_1595 at 1873.781 kb on + strand, within Shewana3_1595 at 1873.878 kb on + strand, within Shewana3_1595 at 1874.148 kb on - strand at 1874.462 kb on - strand at 1874.486 kb on + strand at 1874.487 kb on + strand at 1874.496 kb on - strand at 1875.573 kb on - strand, within Shewana3_1597 at 1875.827 kb on - strand at 1875.827 kb on - strand at 1875.827 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Leucine (C) remove 1,873,537 + Shewana3_1595 0.75 +0.3 1,873,545 + Shewana3_1595 0.75 +0.6 1,873,580 + Shewana3_1595 0.77 -0.8 1,873,588 - Shewana3_1595 0.77 -0.9 1,873,781 + Shewana3_1595 0.84 -0.9 1,873,878 + Shewana3_1595 0.87 -0.2 1,874,148 - +1.9 1,874,462 - +1.1 1,874,486 + -1.1 1,874,487 + +0.3 1,874,496 - -1.0 1,875,573 - Shewana3_1597 0.68 -2.8 1,875,827 - +0.5 1,875,827 - +0.9 1,875,827 - -1.6
Or see this region's nucleotide sequence