Strain Fitness in Shewanella loihica PV-4 around Shew_2255

Experiment: D,L-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntShew_2254 and Shew_2255 are separated by 48 nucleotidesShew_2255 and trpA overlap by 8 nucleotides Shew_2254: Shew_2254 - bifunctional indole-3-glycerol phosphate synthase/phosphoribosylanthranilate isomerase (RefSeq), at 2,633,363 to 2,634,814 _2254 Shew_2255: Shew_2255 - tryptophan synthase subunit beta (RefSeq), at 2,634,863 to 2,636,074 _2255 Shew_2256: trpA - tryptophan synthase subunit alpha (RefSeq), at 2,636,067 to 2,636,870 trpA Position (kb) 2634 2635 2636 2637Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 2634.032 kb on - strand, within Shew_2254at 2634.164 kb on - strand, within Shew_2254at 2634.458 kb on - strand, within Shew_2254at 2634.537 kb on - strand, within Shew_2254at 2634.824 kb on + strandat 2634.941 kb on - strandat 2635.100 kb on - strand, within Shew_2255at 2635.220 kb on + strand, within Shew_2255at 2635.220 kb on + strand, within Shew_2255at 2635.228 kb on - strand, within Shew_2255at 2635.228 kb on - strand, within Shew_2255at 2635.228 kb on - strand, within Shew_2255at 2635.241 kb on + strand, within Shew_2255at 2635.243 kb on - strand, within Shew_2255at 2635.243 kb on - strand, within Shew_2255at 2635.263 kb on - strand, within Shew_2255at 2635.264 kb on - strand, within Shew_2255at 2635.325 kb on + strand, within Shew_2255at 2635.758 kb on - strand, within Shew_2255at 2635.769 kb on + strand, within Shew_2255at 2635.777 kb on - strand, within Shew_2255at 2635.909 kb on + strand, within Shew_2255at 2636.266 kb on + strand, within trpAat 2636.393 kb on - strand, within trpAat 2636.576 kb on + strand, within trpAat 2636.582 kb on + strand, within trpAat 2636.590 kb on - strand, within trpAat 2636.590 kb on - strand, within trpAat 2636.627 kb on + strand, within trpAat 2636.674 kb on - strand, within trpAat 2636.734 kb on - strand, within trpAat 2636.817 kb on + strandat 2636.873 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D,L-Lactate (C)
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2,634,032 - Shew_2254 0.46 -3.1
2,634,164 - Shew_2254 0.55 -3.2
2,634,458 - Shew_2254 0.75 -3.7
2,634,537 - Shew_2254 0.81 -4.1
2,634,824 + -2.9
2,634,941 - -2.8
2,635,100 - Shew_2255 0.20 -5.3
2,635,220 + Shew_2255 0.29 -4.6
2,635,220 + Shew_2255 0.29 -4.3
2,635,228 - Shew_2255 0.30 -5.0
2,635,228 - Shew_2255 0.30 -5.7
2,635,228 - Shew_2255 0.30 -4.1
2,635,241 + Shew_2255 0.31 -3.3
2,635,243 - Shew_2255 0.31 -3.9
2,635,243 - Shew_2255 0.31 -4.0
2,635,263 - Shew_2255 0.33 -3.1
2,635,264 - Shew_2255 0.33 -0.8
2,635,325 + Shew_2255 0.38 -7.1
2,635,758 - Shew_2255 0.74 -4.1
2,635,769 + Shew_2255 0.75 -5.3
2,635,777 - Shew_2255 0.75 -3.0
2,635,909 + Shew_2255 0.86 -4.3
2,636,266 + trpA Shew_2256 0.25 -7.0
2,636,393 - trpA Shew_2256 0.41 -4.0
2,636,576 + trpA Shew_2256 0.63 -4.9
2,636,582 + trpA Shew_2256 0.64 -4.2
2,636,590 - trpA Shew_2256 0.65 -1.4
2,636,590 - trpA Shew_2256 0.65 -5.5
2,636,627 + trpA Shew_2256 0.70 -3.0
2,636,674 - trpA Shew_2256 0.75 -6.6
2,636,734 - trpA Shew_2256 0.83 -4.7
2,636,817 + -7.5
2,636,873 - -0.6

Or see this region's nucleotide sequence