Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_0334 and sdhB overlap by 4 nucleotides sdhB and Shew_0336 are separated by 191 nucleotides Shew_0336 and rho are separated by 75 nucleotides
Shew_0334: Shew_0334 - fumarate reductase flavoprotein subunit (RefSeq), at 378,664 to 380,670
_0334
Shew_0335: sdhB - fumarate reductase iron-sulfur subunit (RefSeq), at 380,667 to 381,398
sdhB
Shew_0336: Shew_0336 - LysR family transcriptional regulator (RefSeq), at 381,590 to 382,513
_0336
Shew_0337: rho - transcription termination factor Rho (RefSeq), at 382,589 to 383,854
rho
Position (kb) 381
382
383 Strain fitness (log2 ratio) -2
-1
0
1 at 380.607 kb on + strand at 380.678 kb on - strand at 380.915 kb on - strand, within sdhB at 380.999 kb on + strand, within sdhB at 381.010 kb on + strand, within sdhB at 381.155 kb on - strand, within sdhB at 381.750 kb on + strand, within Shew_0336 at 381.758 kb on - strand, within Shew_0336 at 381.758 kb on - strand, within Shew_0336 at 381.758 kb on - strand, within Shew_0336 at 381.760 kb on + strand, within Shew_0336 at 381.870 kb on + strand, within Shew_0336 at 381.908 kb on - strand, within Shew_0336 at 382.034 kb on - strand, within Shew_0336 at 382.106 kb on + strand, within Shew_0336 at 382.217 kb on - strand, within Shew_0336 at 382.221 kb on + strand, within Shew_0336 at 382.221 kb on + strand, within Shew_0336 at 382.425 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 380,607 + +0.2 380,678 - +0.2 380,915 - sdhB Shew_0335 0.34 +0.3 380,999 + sdhB Shew_0335 0.45 +0.2 381,010 + sdhB Shew_0335 0.47 +1.7 381,155 - sdhB Shew_0335 0.67 -1.3 381,750 + Shew_0336 0.17 -0.3 381,758 - Shew_0336 0.18 +0.1 381,758 - Shew_0336 0.18 +0.5 381,758 - Shew_0336 0.18 +0.9 381,760 + Shew_0336 0.18 -1.2 381,870 + Shew_0336 0.30 +0.3 381,908 - Shew_0336 0.34 +1.7 382,034 - Shew_0336 0.48 +0.6 382,106 + Shew_0336 0.56 -0.1 382,217 - Shew_0336 0.68 +0.6 382,221 + Shew_0336 0.68 +0.5 382,221 + Shew_0336 0.68 +0.0 382,425 + -2.1
Or see this region's nucleotide sequence