Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS18095 and CA265_RS18100 are separated by 77 nucleotides CA265_RS18100 and CA265_RS18105 are separated by 2 nucleotides CA265_RS18105 and CA265_RS18110 are separated by 32 nucleotides CA265_RS18110 and CA265_RS18115 are separated by 108 nucleotides
CA265_RS18095: CA265_RS18095 - peptidase, at 4,517,308 to 4,519,527
_RS18095
CA265_RS18100: CA265_RS18100 - hypothetical protein, at 4,519,605 to 4,519,835
_RS18100
CA265_RS18105: CA265_RS18105 - hypothetical protein, at 4,519,838 to 4,520,206
_RS18105
CA265_RS18110: CA265_RS18110 - excinuclease ABC subunit C, at 4,520,239 to 4,522,041
_RS18110
CA265_RS18115: CA265_RS18115 - gliding motility protein GldN, at 4,522,150 to 4,523,064
_RS18115
Position (kb)
4520
4521
4522
4523 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4519.459 kb on + strand at 4519.525 kb on + strand at 4519.530 kb on - strand at 4519.530 kb on - strand at 4519.534 kb on + strand at 4519.802 kb on + strand, within CA265_RS18100 at 4519.804 kb on + strand, within CA265_RS18100 at 4519.812 kb on - strand, within CA265_RS18100 at 4519.856 kb on - strand at 4519.858 kb on + strand at 4519.866 kb on + strand at 4519.883 kb on - strand, within CA265_RS18105 at 4519.915 kb on - strand, within CA265_RS18105 at 4519.945 kb on + strand, within CA265_RS18105 at 4519.978 kb on + strand, within CA265_RS18105 at 4519.979 kb on - strand, within CA265_RS18105 at 4519.979 kb on - strand, within CA265_RS18105 at 4519.979 kb on - strand, within CA265_RS18105 at 4520.016 kb on - strand, within CA265_RS18105 at 4520.024 kb on - strand, within CA265_RS18105 at 4520.042 kb on + strand, within CA265_RS18105 at 4520.043 kb on - strand, within CA265_RS18105 at 4520.043 kb on - strand, within CA265_RS18105 at 4520.044 kb on + strand, within CA265_RS18105 at 4520.044 kb on + strand, within CA265_RS18105 at 4520.044 kb on + strand, within CA265_RS18105 at 4520.045 kb on - strand, within CA265_RS18105 at 4520.045 kb on - strand, within CA265_RS18105 at 4520.045 kb on - strand, within CA265_RS18105 at 4520.045 kb on - strand, within CA265_RS18105 at 4520.045 kb on - strand, within CA265_RS18105 at 4520.047 kb on - strand, within CA265_RS18105 at 4520.047 kb on - strand, within CA265_RS18105 at 4520.047 kb on - strand, within CA265_RS18105 at 4520.047 kb on - strand, within CA265_RS18105 at 4520.086 kb on - strand, within CA265_RS18105 at 4520.156 kb on - strand, within CA265_RS18105 at 4520.160 kb on - strand, within CA265_RS18105 at 4520.194 kb on + strand at 4520.201 kb on + strand at 4520.230 kb on + strand at 4520.283 kb on + strand at 4520.283 kb on + strand at 4520.297 kb on - strand at 4520.329 kb on + strand at 4520.344 kb on - strand at 4520.372 kb on - strand at 4520.415 kb on - strand at 4520.434 kb on - strand, within CA265_RS18110 at 4520.619 kb on + strand, within CA265_RS18110 at 4520.619 kb on + strand, within CA265_RS18110 at 4520.620 kb on - strand, within CA265_RS18110 at 4520.620 kb on - strand, within CA265_RS18110 at 4520.658 kb on + strand, within CA265_RS18110 at 4520.658 kb on + strand, within CA265_RS18110 at 4520.659 kb on - strand, within CA265_RS18110 at 4520.659 kb on - strand, within CA265_RS18110 at 4520.687 kb on - strand, within CA265_RS18110 at 4520.762 kb on - strand, within CA265_RS18110 at 4520.778 kb on + strand, within CA265_RS18110 at 4520.779 kb on - strand, within CA265_RS18110 at 4520.828 kb on + strand, within CA265_RS18110 at 4520.938 kb on + strand, within CA265_RS18110 at 4520.938 kb on + strand, within CA265_RS18110 at 4520.951 kb on + strand, within CA265_RS18110 at 4520.952 kb on - strand, within CA265_RS18110 at 4520.968 kb on + strand, within CA265_RS18110 at 4520.969 kb on - strand, within CA265_RS18110 at 4520.991 kb on - strand, within CA265_RS18110 at 4521.011 kb on + strand, within CA265_RS18110 at 4521.011 kb on + strand, within CA265_RS18110 at 4521.011 kb on + strand, within CA265_RS18110 at 4521.011 kb on + strand, within CA265_RS18110 at 4521.059 kb on - strand, within CA265_RS18110 at 4521.108 kb on + strand, within CA265_RS18110 at 4521.222 kb on - strand, within CA265_RS18110 at 4521.299 kb on + strand, within CA265_RS18110 at 4521.331 kb on + strand, within CA265_RS18110 at 4521.331 kb on + strand, within CA265_RS18110 at 4521.419 kb on + strand, within CA265_RS18110 at 4521.419 kb on + strand, within CA265_RS18110 at 4521.419 kb on + strand, within CA265_RS18110 at 4521.420 kb on - strand, within CA265_RS18110 at 4521.481 kb on - strand, within CA265_RS18110 at 4521.544 kb on - strand, within CA265_RS18110 at 4521.558 kb on + strand, within CA265_RS18110 at 4521.583 kb on + strand, within CA265_RS18110 at 4521.681 kb on - strand, within CA265_RS18110 at 4521.742 kb on + strand, within CA265_RS18110 at 4521.766 kb on + strand, within CA265_RS18110 at 4521.767 kb on - strand, within CA265_RS18110 at 4521.837 kb on + strand, within CA265_RS18110 at 4522.042 kb on - strand at 4522.048 kb on + strand at 4522.090 kb on - strand at 4522.090 kb on - strand at 4522.106 kb on - strand at 4522.144 kb on + strand at 4522.148 kb on - strand at 4522.152 kb on - strand at 4522.155 kb on - strand at 4522.211 kb on + strand at 4522.236 kb on + strand at 4522.237 kb on - strand at 4522.240 kb on - strand at 4522.277 kb on - strand, within CA265_RS18115 at 4522.284 kb on - strand, within CA265_RS18115 at 4522.284 kb on - strand, within CA265_RS18115 at 4522.294 kb on + strand, within CA265_RS18115 at 4522.392 kb on - strand, within CA265_RS18115 at 4522.394 kb on + strand, within CA265_RS18115 at 4522.451 kb on - strand, within CA265_RS18115 at 4522.451 kb on - strand, within CA265_RS18115 at 4522.451 kb on - strand, within CA265_RS18115 at 4522.452 kb on + strand, within CA265_RS18115 at 4522.452 kb on + strand, within CA265_RS18115 at 4522.452 kb on + strand, within CA265_RS18115 at 4522.453 kb on - strand, within CA265_RS18115 at 4522.457 kb on - strand, within CA265_RS18115 at 4522.457 kb on - strand, within CA265_RS18115 at 4522.461 kb on + strand, within CA265_RS18115 at 4522.462 kb on - strand, within CA265_RS18115 at 4522.528 kb on + strand, within CA265_RS18115 at 4522.548 kb on + strand, within CA265_RS18115 at 4522.553 kb on - strand, within CA265_RS18115 at 4522.683 kb on - strand, within CA265_RS18115 at 4522.683 kb on - strand, within CA265_RS18115 at 4522.718 kb on - strand, within CA265_RS18115 at 4522.720 kb on + strand, within CA265_RS18115 at 4522.721 kb on - strand, within CA265_RS18115 at 4522.761 kb on + strand, within CA265_RS18115 at 4522.775 kb on - strand, within CA265_RS18115 at 4522.888 kb on - strand, within CA265_RS18115 at 4522.899 kb on + strand, within CA265_RS18115 at 4522.920 kb on + strand, within CA265_RS18115 at 4522.964 kb on - strand, within CA265_RS18115 at 4522.995 kb on + strand at 4523.027 kb on - strand at 4523.040 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 4,519,459 + +0.1 4,519,525 + +0.3 4,519,530 - -0.3 4,519,530 - -1.5 4,519,534 + +0.2 4,519,802 + CA265_RS18100 0.85 +0.0 4,519,804 + CA265_RS18100 0.86 -2.1 4,519,812 - CA265_RS18100 0.90 +0.2 4,519,856 - +0.1 4,519,858 + +0.8 4,519,866 + +0.2 4,519,883 - CA265_RS18105 0.12 +0.5 4,519,915 - CA265_RS18105 0.21 +0.4 4,519,945 + CA265_RS18105 0.29 -1.3 4,519,978 + CA265_RS18105 0.38 -0.6 4,519,979 - CA265_RS18105 0.38 +1.5 4,519,979 - CA265_RS18105 0.38 -0.1 4,519,979 - CA265_RS18105 0.38 +1.2 4,520,016 - CA265_RS18105 0.48 -0.0 4,520,024 - CA265_RS18105 0.50 +0.3 4,520,042 + CA265_RS18105 0.55 -0.2 4,520,043 - CA265_RS18105 0.56 +0.7 4,520,043 - CA265_RS18105 0.56 +0.5 4,520,044 + CA265_RS18105 0.56 -1.4 4,520,044 + CA265_RS18105 0.56 +0.0 4,520,044 + CA265_RS18105 0.56 +0.3 4,520,045 - CA265_RS18105 0.56 +0.2 4,520,045 - CA265_RS18105 0.56 -0.4 4,520,045 - CA265_RS18105 0.56 -0.9 4,520,045 - CA265_RS18105 0.56 -0.4 4,520,045 - CA265_RS18105 0.56 -0.1 4,520,047 - CA265_RS18105 0.57 -0.5 4,520,047 - CA265_RS18105 0.57 -0.2 4,520,047 - CA265_RS18105 0.57 +0.5 4,520,047 - CA265_RS18105 0.57 +0.3 4,520,086 - CA265_RS18105 0.67 +2.1 4,520,156 - CA265_RS18105 0.86 -0.3 4,520,160 - CA265_RS18105 0.87 -0.3 4,520,194 + +1.1 4,520,201 + -0.6 4,520,230 + +0.3 4,520,283 + +1.0 4,520,283 + -1.1 4,520,297 - +1.0 4,520,329 + +0.1 4,520,344 - +0.1 4,520,372 - +0.2 4,520,415 - -0.5 4,520,434 - CA265_RS18110 0.11 +0.9 4,520,619 + CA265_RS18110 0.21 +1.2 4,520,619 + CA265_RS18110 0.21 -2.3 4,520,620 - CA265_RS18110 0.21 +0.9 4,520,620 - CA265_RS18110 0.21 +0.5 4,520,658 + CA265_RS18110 0.23 +0.2 4,520,658 + CA265_RS18110 0.23 +0.9 4,520,659 - CA265_RS18110 0.23 +2.5 4,520,659 - CA265_RS18110 0.23 +0.2 4,520,687 - CA265_RS18110 0.25 +0.4 4,520,762 - CA265_RS18110 0.29 +0.4 4,520,778 + CA265_RS18110 0.30 +0.7 4,520,779 - CA265_RS18110 0.30 +0.2 4,520,828 + CA265_RS18110 0.33 -0.8 4,520,938 + CA265_RS18110 0.39 -0.8 4,520,938 + CA265_RS18110 0.39 +0.8 4,520,951 + CA265_RS18110 0.39 -0.2 4,520,952 - CA265_RS18110 0.40 +0.8 4,520,968 + CA265_RS18110 0.40 +0.9 4,520,969 - CA265_RS18110 0.40 +2.1 4,520,991 - CA265_RS18110 0.42 +0.3 4,521,011 + CA265_RS18110 0.43 +0.2 4,521,011 + CA265_RS18110 0.43 +0.4 4,521,011 + CA265_RS18110 0.43 +0.4 4,521,011 + CA265_RS18110 0.43 -0.7 4,521,059 - CA265_RS18110 0.45 +0.1 4,521,108 + CA265_RS18110 0.48 +0.6 4,521,222 - CA265_RS18110 0.55 +0.7 4,521,299 + CA265_RS18110 0.59 +0.5 4,521,331 + CA265_RS18110 0.61 +0.2 4,521,331 + CA265_RS18110 0.61 -0.1 4,521,419 + CA265_RS18110 0.65 +0.1 4,521,419 + CA265_RS18110 0.65 +0.4 4,521,419 + CA265_RS18110 0.65 +2.1 4,521,420 - CA265_RS18110 0.66 +0.6 4,521,481 - CA265_RS18110 0.69 +0.5 4,521,544 - CA265_RS18110 0.72 -0.1 4,521,558 + CA265_RS18110 0.73 +0.3 4,521,583 + CA265_RS18110 0.75 +0.5 4,521,681 - CA265_RS18110 0.80 +0.5 4,521,742 + CA265_RS18110 0.83 -1.5 4,521,766 + CA265_RS18110 0.85 -0.7 4,521,767 - CA265_RS18110 0.85 +0.2 4,521,837 + CA265_RS18110 0.89 -1.6 4,522,042 - +0.2 4,522,048 + +0.4 4,522,090 - +0.8 4,522,090 - -0.1 4,522,106 - -1.8 4,522,144 + -0.4 4,522,148 - -1.1 4,522,152 - -1.2 4,522,155 - +0.8 4,522,211 + +0.9 4,522,236 + +1.2 4,522,237 - +0.1 4,522,240 - +1.2 4,522,277 - CA265_RS18115 0.14 +0.2 4,522,284 - CA265_RS18115 0.15 +1.0 4,522,284 - CA265_RS18115 0.15 +1.2 4,522,294 + CA265_RS18115 0.16 +1.1 4,522,392 - CA265_RS18115 0.26 -0.0 4,522,394 + CA265_RS18115 0.27 +0.7 4,522,451 - CA265_RS18115 0.33 +0.5 4,522,451 - CA265_RS18115 0.33 -0.3 4,522,451 - CA265_RS18115 0.33 +0.0 4,522,452 + CA265_RS18115 0.33 +0.9 4,522,452 + CA265_RS18115 0.33 -1.4 4,522,452 + CA265_RS18115 0.33 -0.9 4,522,453 - CA265_RS18115 0.33 +1.9 4,522,457 - CA265_RS18115 0.34 -0.3 4,522,457 - CA265_RS18115 0.34 +0.8 4,522,461 + CA265_RS18115 0.34 +0.3 4,522,462 - CA265_RS18115 0.34 +0.7 4,522,528 + CA265_RS18115 0.41 -0.3 4,522,548 + CA265_RS18115 0.43 +1.0 4,522,553 - CA265_RS18115 0.44 +0.0 4,522,683 - CA265_RS18115 0.58 -0.2 4,522,683 - CA265_RS18115 0.58 +0.7 4,522,718 - CA265_RS18115 0.62 +0.3 4,522,720 + CA265_RS18115 0.62 +0.1 4,522,721 - CA265_RS18115 0.62 +0.3 4,522,761 + CA265_RS18115 0.67 +1.4 4,522,775 - CA265_RS18115 0.68 +0.4 4,522,888 - CA265_RS18115 0.81 -1.1 4,522,899 + CA265_RS18115 0.82 +0.1 4,522,920 + CA265_RS18115 0.84 +0.5 4,522,964 - CA265_RS18115 0.89 +0.4 4,522,995 + +0.3 4,523,027 - +0.6 4,523,040 + +2.1
Or see this region's nucleotide sequence