Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS05100 and CA265_RS05105 are separated by 83 nucleotides CA265_RS05105 and CA265_RS05110 are separated by 405 nucleotides
CA265_RS05100: CA265_RS05100 - threonylcarbamoyl-AMP synthase, at 1,215,865 to 1,216,485
_RS05100
CA265_RS05105: CA265_RS05105 - FMN-binding glutamate synthase family protein, at 1,216,569 to 1,218,230
_RS05105
CA265_RS05110: CA265_RS05110 - lantibiotic ABC transporter, at 1,218,636 to 1,219,622
_RS05110
Position (kb)
1216
1217
1218
1219 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1215.600 kb on + strand at 1215.612 kb on + strand at 1215.613 kb on - strand at 1215.613 kb on - strand at 1215.613 kb on - strand at 1215.654 kb on + strand at 1215.655 kb on - strand at 1215.658 kb on - strand at 1215.699 kb on + strand at 1215.758 kb on + strand at 1215.759 kb on - strand at 1215.821 kb on - strand at 1215.853 kb on + strand at 1215.866 kb on + strand at 1215.882 kb on + strand at 1216.062 kb on + strand, within CA265_RS05100 at 1216.062 kb on + strand, within CA265_RS05100 at 1216.107 kb on + strand, within CA265_RS05100 at 1216.107 kb on + strand, within CA265_RS05100 at 1216.107 kb on + strand, within CA265_RS05100 at 1216.108 kb on - strand, within CA265_RS05100 at 1216.171 kb on - strand, within CA265_RS05100 at 1216.194 kb on + strand, within CA265_RS05100 at 1216.236 kb on + strand, within CA265_RS05100 at 1216.237 kb on - strand, within CA265_RS05100 at 1216.254 kb on + strand, within CA265_RS05100 at 1216.257 kb on - strand, within CA265_RS05100 at 1216.402 kb on - strand, within CA265_RS05100 at 1216.405 kb on + strand, within CA265_RS05100 at 1216.405 kb on + strand, within CA265_RS05100 at 1216.406 kb on - strand, within CA265_RS05100 at 1216.471 kb on + strand at 1216.472 kb on - strand at 1216.472 kb on - strand at 1216.485 kb on + strand at 1216.485 kb on + strand at 1216.486 kb on - strand at 1216.505 kb on - strand at 1216.569 kb on - strand at 1216.646 kb on - strand at 1216.702 kb on - strand at 1216.715 kb on + strand at 1216.716 kb on - strand at 1216.716 kb on - strand at 1216.718 kb on + strand at 1216.719 kb on - strand at 1216.719 kb on - strand at 1216.719 kb on - strand at 1216.755 kb on + strand, within CA265_RS05105 at 1216.785 kb on + strand, within CA265_RS05105 at 1216.817 kb on + strand, within CA265_RS05105 at 1216.825 kb on - strand, within CA265_RS05105 at 1216.825 kb on - strand, within CA265_RS05105 at 1216.834 kb on + strand, within CA265_RS05105 at 1216.901 kb on - strand, within CA265_RS05105 at 1216.931 kb on - strand, within CA265_RS05105 at 1216.937 kb on + strand, within CA265_RS05105 at 1216.937 kb on + strand, within CA265_RS05105 at 1217.032 kb on - strand, within CA265_RS05105 at 1217.062 kb on + strand, within CA265_RS05105 at 1217.100 kb on - strand, within CA265_RS05105 at 1217.120 kb on + strand, within CA265_RS05105 at 1217.229 kb on - strand, within CA265_RS05105 at 1217.233 kb on - strand, within CA265_RS05105 at 1217.241 kb on + strand, within CA265_RS05105 at 1217.242 kb on - strand, within CA265_RS05105 at 1217.242 kb on - strand, within CA265_RS05105 at 1217.390 kb on - strand, within CA265_RS05105 at 1217.390 kb on - strand, within CA265_RS05105 at 1217.421 kb on - strand, within CA265_RS05105 at 1217.662 kb on + strand, within CA265_RS05105 at 1217.662 kb on + strand, within CA265_RS05105 at 1217.663 kb on - strand, within CA265_RS05105 at 1217.663 kb on - strand, within CA265_RS05105 at 1217.665 kb on - strand, within CA265_RS05105 at 1217.753 kb on + strand, within CA265_RS05105 at 1217.806 kb on + strand, within CA265_RS05105 at 1217.806 kb on + strand, within CA265_RS05105 at 1217.840 kb on + strand, within CA265_RS05105 at 1217.840 kb on + strand, within CA265_RS05105 at 1217.960 kb on - strand, within CA265_RS05105 at 1217.962 kb on + strand, within CA265_RS05105 at 1217.963 kb on - strand, within CA265_RS05105 at 1218.006 kb on + strand, within CA265_RS05105 at 1218.006 kb on + strand, within CA265_RS05105 at 1218.022 kb on + strand, within CA265_RS05105 at 1218.034 kb on + strand, within CA265_RS05105 at 1218.045 kb on + strand, within CA265_RS05105 at 1218.063 kb on + strand, within CA265_RS05105 at 1218.077 kb on + strand at 1218.078 kb on - strand at 1218.078 kb on - strand at 1218.132 kb on - strand at 1218.153 kb on - strand at 1218.180 kb on + strand at 1218.181 kb on - strand at 1218.192 kb on - strand at 1218.192 kb on - strand at 1218.209 kb on + strand at 1218.209 kb on + strand at 1218.209 kb on + strand at 1218.210 kb on - strand at 1218.210 kb on - strand at 1218.210 kb on - strand at 1218.284 kb on - strand at 1218.284 kb on - strand at 1218.326 kb on - strand at 1218.326 kb on - strand at 1218.419 kb on + strand at 1218.420 kb on - strand at 1218.431 kb on - strand at 1218.518 kb on - strand at 1218.565 kb on - strand at 1218.609 kb on + strand at 1218.611 kb on + strand at 1218.652 kb on + strand at 1218.662 kb on + strand at 1218.772 kb on - strand, within CA265_RS05110 at 1218.804 kb on - strand, within CA265_RS05110 at 1218.805 kb on + strand, within CA265_RS05110 at 1218.806 kb on - strand, within CA265_RS05110 at 1218.913 kb on + strand, within CA265_RS05110 at 1218.944 kb on - strand, within CA265_RS05110 at 1218.964 kb on - strand, within CA265_RS05110 at 1219.008 kb on + strand, within CA265_RS05110 at 1219.021 kb on - strand, within CA265_RS05110 at 1219.051 kb on + strand, within CA265_RS05110 at 1219.061 kb on - strand, within CA265_RS05110 at 1219.061 kb on - strand, within CA265_RS05110 at 1219.127 kb on + strand, within CA265_RS05110 at 1219.127 kb on + strand, within CA265_RS05110 at 1219.128 kb on - strand, within CA265_RS05110 at 1219.128 kb on - strand, within CA265_RS05110 at 1219.150 kb on - strand, within CA265_RS05110
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 1,215,600 + +0.4 1,215,612 + +0.4 1,215,613 - -0.2 1,215,613 - -0.8 1,215,613 - -0.5 1,215,654 + +0.1 1,215,655 - -1.6 1,215,658 - -2.0 1,215,699 + -1.5 1,215,758 + +1.1 1,215,759 - +0.7 1,215,821 - -0.3 1,215,853 + +0.1 1,215,866 + +0.1 1,215,882 + -0.3 1,216,062 + CA265_RS05100 0.32 -1.4 1,216,062 + CA265_RS05100 0.32 +0.3 1,216,107 + CA265_RS05100 0.39 +0.0 1,216,107 + CA265_RS05100 0.39 +0.5 1,216,107 + CA265_RS05100 0.39 +0.5 1,216,108 - CA265_RS05100 0.39 -0.0 1,216,171 - CA265_RS05100 0.49 -0.1 1,216,194 + CA265_RS05100 0.53 +0.3 1,216,236 + CA265_RS05100 0.60 +0.9 1,216,237 - CA265_RS05100 0.60 +0.4 1,216,254 + CA265_RS05100 0.63 -0.5 1,216,257 - CA265_RS05100 0.63 +0.2 1,216,402 - CA265_RS05100 0.86 -0.2 1,216,405 + CA265_RS05100 0.87 +0.1 1,216,405 + CA265_RS05100 0.87 +1.0 1,216,406 - CA265_RS05100 0.87 -0.4 1,216,471 + -0.0 1,216,472 - +0.6 1,216,472 - -0.2 1,216,485 + -0.0 1,216,485 + -0.7 1,216,486 - -0.1 1,216,505 - -0.2 1,216,569 - +0.6 1,216,646 - +0.2 1,216,702 - -1.0 1,216,715 + -0.3 1,216,716 - -1.7 1,216,716 - +0.1 1,216,718 + +0.4 1,216,719 - -0.1 1,216,719 - -1.0 1,216,719 - +0.4 1,216,755 + CA265_RS05105 0.11 -0.2 1,216,785 + CA265_RS05105 0.13 +0.5 1,216,817 + CA265_RS05105 0.15 +0.6 1,216,825 - CA265_RS05105 0.15 +1.1 1,216,825 - CA265_RS05105 0.15 -0.1 1,216,834 + CA265_RS05105 0.16 -0.6 1,216,901 - CA265_RS05105 0.20 -0.0 1,216,931 - CA265_RS05105 0.22 +0.1 1,216,937 + CA265_RS05105 0.22 +1.1 1,216,937 + CA265_RS05105 0.22 -0.0 1,217,032 - CA265_RS05105 0.28 -0.2 1,217,062 + CA265_RS05105 0.30 +0.5 1,217,100 - CA265_RS05105 0.32 +0.7 1,217,120 + CA265_RS05105 0.33 +0.2 1,217,229 - CA265_RS05105 0.40 -0.0 1,217,233 - CA265_RS05105 0.40 -0.2 1,217,241 + CA265_RS05105 0.40 +0.6 1,217,242 - CA265_RS05105 0.40 +0.8 1,217,242 - CA265_RS05105 0.40 +0.9 1,217,390 - CA265_RS05105 0.49 +0.3 1,217,390 - CA265_RS05105 0.49 +0.2 1,217,421 - CA265_RS05105 0.51 +0.1 1,217,662 + CA265_RS05105 0.66 +0.1 1,217,662 + CA265_RS05105 0.66 -0.7 1,217,663 - CA265_RS05105 0.66 -0.5 1,217,663 - CA265_RS05105 0.66 -0.3 1,217,665 - CA265_RS05105 0.66 +0.7 1,217,753 + CA265_RS05105 0.71 -0.4 1,217,806 + CA265_RS05105 0.74 +0.5 1,217,806 + CA265_RS05105 0.74 -0.3 1,217,840 + CA265_RS05105 0.76 +0.5 1,217,840 + CA265_RS05105 0.76 +0.5 1,217,960 - CA265_RS05105 0.84 -0.4 1,217,962 + CA265_RS05105 0.84 +0.4 1,217,963 - CA265_RS05105 0.84 +0.7 1,218,006 + CA265_RS05105 0.86 +0.2 1,218,006 + CA265_RS05105 0.86 +0.4 1,218,022 + CA265_RS05105 0.87 +1.0 1,218,034 + CA265_RS05105 0.88 -0.4 1,218,045 + CA265_RS05105 0.89 +1.1 1,218,063 + CA265_RS05105 0.90 -0.2 1,218,077 + -0.1 1,218,078 - +0.5 1,218,078 - -0.2 1,218,132 - -0.5 1,218,153 - +0.3 1,218,180 + +0.6 1,218,181 - +0.2 1,218,192 - -0.0 1,218,192 - +2.6 1,218,209 + -0.3 1,218,209 + +1.0 1,218,209 + -0.4 1,218,210 - -1.1 1,218,210 - -0.4 1,218,210 - -0.2 1,218,284 - -0.6 1,218,284 - +0.7 1,218,326 - +0.7 1,218,326 - -0.2 1,218,419 + -0.5 1,218,420 - -0.7 1,218,431 - +0.1 1,218,518 - -0.3 1,218,565 - -0.2 1,218,609 + +0.8 1,218,611 + +1.4 1,218,652 + -1.4 1,218,662 + +0.2 1,218,772 - CA265_RS05110 0.14 +0.0 1,218,804 - CA265_RS05110 0.17 -0.1 1,218,805 + CA265_RS05110 0.17 +1.5 1,218,806 - CA265_RS05110 0.17 +0.1 1,218,913 + CA265_RS05110 0.28 +0.2 1,218,944 - CA265_RS05110 0.31 -1.1 1,218,964 - CA265_RS05110 0.33 +0.4 1,219,008 + CA265_RS05110 0.38 +1.4 1,219,021 - CA265_RS05110 0.39 -0.5 1,219,051 + CA265_RS05110 0.42 +1.0 1,219,061 - CA265_RS05110 0.43 -1.1 1,219,061 - CA265_RS05110 0.43 +0.4 1,219,127 + CA265_RS05110 0.50 -0.6 1,219,127 + CA265_RS05110 0.50 +0.6 1,219,128 - CA265_RS05110 0.50 -0.4 1,219,128 - CA265_RS05110 0.50 -0.1 1,219,150 - CA265_RS05110 0.52 +1.1
Or see this region's nucleotide sequence