Experiment: R2A with Lomefloxacin 0.1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02205 and CA265_RS02210 are separated by 219 nucleotides CA265_RS02210 and CA265_RS02215 are separated by 63 nucleotides
CA265_RS02205: CA265_RS02205 - N-acetylglucosamine kinase, at 557,206 to 558,066
_RS02205
CA265_RS02210: CA265_RS02210 - threonine aldolase, at 558,286 to 559,311
_RS02210
CA265_RS02215: CA265_RS02215 - acetyl-CoA carboxylase biotin carboxylase subunit, at 559,375 to 560,886
_RS02215
Position (kb) 558
559
560 Strain fitness (log2 ratio) -2
-1
0
1
2 at 557.549 kb on + strand, within CA265_RS02205 at 557.553 kb on + strand, within CA265_RS02205 at 557.589 kb on - strand, within CA265_RS02205 at 557.877 kb on - strand, within CA265_RS02205 at 557.942 kb on - strand, within CA265_RS02205 at 558.039 kb on + strand at 558.059 kb on - strand at 558.182 kb on + strand at 558.438 kb on + strand, within CA265_RS02210 at 558.455 kb on + strand, within CA265_RS02210 at 558.495 kb on + strand, within CA265_RS02210 at 558.548 kb on + strand, within CA265_RS02210 at 558.606 kb on - strand, within CA265_RS02210 at 558.606 kb on - strand, within CA265_RS02210 at 558.607 kb on + strand, within CA265_RS02210 at 558.664 kb on - strand, within CA265_RS02210 at 558.699 kb on - strand, within CA265_RS02210 at 558.714 kb on + strand, within CA265_RS02210 at 558.715 kb on - strand, within CA265_RS02210 at 558.715 kb on - strand, within CA265_RS02210 at 558.735 kb on + strand, within CA265_RS02210 at 558.736 kb on - strand, within CA265_RS02210 at 558.757 kb on - strand, within CA265_RS02210 at 558.776 kb on + strand, within CA265_RS02210 at 558.777 kb on - strand, within CA265_RS02210 at 558.777 kb on - strand, within CA265_RS02210 at 558.786 kb on - strand, within CA265_RS02210 at 558.854 kb on - strand, within CA265_RS02210 at 558.862 kb on + strand, within CA265_RS02210 at 559.004 kb on + strand, within CA265_RS02210 at 559.021 kb on + strand, within CA265_RS02210 at 559.079 kb on + strand, within CA265_RS02210 at 559.105 kb on + strand, within CA265_RS02210 at 559.138 kb on - strand, within CA265_RS02210 at 559.138 kb on - strand, within CA265_RS02210 at 559.293 kb on - strand at 559.322 kb on - strand at 559.350 kb on + strand at 559.449 kb on + strand at 559.450 kb on - strand at 559.526 kb on + strand at 559.527 kb on - strand, within CA265_RS02215 at 559.527 kb on - strand, within CA265_RS02215 at 559.579 kb on + strand, within CA265_RS02215 at 559.873 kb on - strand, within CA265_RS02215 at 559.908 kb on - strand, within CA265_RS02215 at 560.005 kb on + strand, within CA265_RS02215 at 560.005 kb on + strand, within CA265_RS02215 at 560.011 kb on - strand, within CA265_RS02215 at 560.011 kb on - strand, within CA265_RS02215 at 560.061 kb on + strand, within CA265_RS02215 at 560.117 kb on + strand, within CA265_RS02215 at 560.181 kb on - strand, within CA265_RS02215 at 560.187 kb on - strand, within CA265_RS02215 at 560.310 kb on + strand, within CA265_RS02215
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Lomefloxacin 0.1 mM remove 557,549 + CA265_RS02205 0.40 -1.7 557,553 + CA265_RS02205 0.40 +1.5 557,589 - CA265_RS02205 0.44 -1.3 557,877 - CA265_RS02205 0.78 -2.2 557,942 - CA265_RS02205 0.85 -0.1 558,039 + -1.7 558,059 - -0.8 558,182 + -0.5 558,438 + CA265_RS02210 0.15 -0.3 558,455 + CA265_RS02210 0.16 +0.5 558,495 + CA265_RS02210 0.20 -0.9 558,548 + CA265_RS02210 0.26 -0.1 558,606 - CA265_RS02210 0.31 +0.5 558,606 - CA265_RS02210 0.31 +1.4 558,607 + CA265_RS02210 0.31 -0.1 558,664 - CA265_RS02210 0.37 -0.5 558,699 - CA265_RS02210 0.40 +0.9 558,714 + CA265_RS02210 0.42 +0.8 558,715 - CA265_RS02210 0.42 +0.3 558,715 - CA265_RS02210 0.42 +0.6 558,735 + CA265_RS02210 0.44 -0.9 558,736 - CA265_RS02210 0.44 +0.1 558,757 - CA265_RS02210 0.46 -0.8 558,776 + CA265_RS02210 0.48 -0.3 558,777 - CA265_RS02210 0.48 +1.2 558,777 - CA265_RS02210 0.48 -0.2 558,786 - CA265_RS02210 0.49 +0.8 558,854 - CA265_RS02210 0.55 +0.3 558,862 + CA265_RS02210 0.56 +0.9 559,004 + CA265_RS02210 0.70 +0.5 559,021 + CA265_RS02210 0.72 +2.6 559,079 + CA265_RS02210 0.77 -0.5 559,105 + CA265_RS02210 0.80 -0.3 559,138 - CA265_RS02210 0.83 +1.1 559,138 - CA265_RS02210 0.83 +0.7 559,293 - +0.2 559,322 - -0.8 559,350 + -1.0 559,449 + -0.5 559,450 - -0.1 559,526 + -0.5 559,527 - CA265_RS02215 0.10 -1.2 559,527 - CA265_RS02215 0.10 -1.1 559,579 + CA265_RS02215 0.13 -1.7 559,873 - CA265_RS02215 0.33 -0.4 559,908 - CA265_RS02215 0.35 +0.5 560,005 + CA265_RS02215 0.42 -0.2 560,005 + CA265_RS02215 0.42 -0.6 560,011 - CA265_RS02215 0.42 -0.4 560,011 - CA265_RS02215 0.42 +0.7 560,061 + CA265_RS02215 0.45 +0.1 560,117 + CA265_RS02215 0.49 -0.2 560,181 - CA265_RS02215 0.53 -0.9 560,187 - CA265_RS02215 0.54 -0.0 560,310 + CA265_RS02215 0.62 -1.1
Or see this region's nucleotide sequence