Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3733 and Shewana3_3734 are separated by 9 nucleotides Shewana3_3734 and Shewana3_R0128 are separated by 232 nucleotides Shewana3_R0128 and Shewana3_R0129 are separated by 125 nucleotides
Shewana3_3733: Shewana3_3733 - diguanylate cyclase (RefSeq), at 4,474,035 to 4,475,912
_3733
Shewana3_3734: Shewana3_3734 - delta-aminolevulinic acid dehydratase (RefSeq), at 4,475,922 to 4,476,932
_3734
Shewana3_R0128: Shewana3_R0128 - 5S ribosomal RNA (RefSeq), at 4,477,165 to 4,477,284
_R0128
Shewana3_R0129: Shewana3_R0129 - 23S ribosomal RNA (RefSeq), at 4,477,410 to 4,480,304
_R0129
Position (kb)
4475
4476
4477 Strain fitness (log2 ratio)
-2
-1
0
1 at 4474.938 kb on - strand, within Shewana3_3733 at 4474.948 kb on - strand, within Shewana3_3733 at 4474.965 kb on + strand, within Shewana3_3733 at 4475.033 kb on + strand, within Shewana3_3733 at 4475.214 kb on - strand, within Shewana3_3733 at 4475.319 kb on + strand, within Shewana3_3733 at 4475.352 kb on - strand, within Shewana3_3733 at 4475.645 kb on - strand, within Shewana3_3733 at 4476.178 kb on - strand, within Shewana3_3734 at 4476.453 kb on + strand, within Shewana3_3734 at 4476.494 kb on - strand, within Shewana3_3734 at 4476.533 kb on - strand, within Shewana3_3734 at 4476.535 kb on + strand, within Shewana3_3734 at 4476.747 kb on + strand, within Shewana3_3734
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 4,474,938 - Shewana3_3733 0.48 -1.3 4,474,948 - Shewana3_3733 0.49 +0.8 4,474,965 + Shewana3_3733 0.50 +0.6 4,475,033 + Shewana3_3733 0.53 +0.5 4,475,214 - Shewana3_3733 0.63 +0.5 4,475,319 + Shewana3_3733 0.68 -1.7 4,475,352 - Shewana3_3733 0.70 +1.2 4,475,645 - Shewana3_3733 0.86 -1.7 4,476,178 - Shewana3_3734 0.25 -1.9 4,476,453 + Shewana3_3734 0.53 -1.6 4,476,494 - Shewana3_3734 0.57 -0.8 4,476,533 - Shewana3_3734 0.60 -1.8 4,476,535 + Shewana3_3734 0.61 -0.2 4,476,747 + Shewana3_3734 0.82 +0.8
Or see this region's nucleotide sequence