Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_3172 and Shewana3_R0121 are separated by 219 nucleotides Shewana3_R0121 and Shewana3_R0122 are separated by 55 nucleotides Shewana3_R0122 and Shewana3_R0123 are separated by 44 nucleotides Shewana3_R0123 and Shewana3_3173 are separated by 163 nucleotides
Shewana3_3172: Shewana3_3172 - putative lipoprotein (RefSeq), at 3,811,687 to 3,812,535
_3172
Shewana3_R0121: Shewana3_R0121 - tRNA-Phe (RefSeq), at 3,812,755 to 3,812,830
_R0121
Shewana3_R0122: Shewana3_R0122 - tRNA-Leu (RefSeq), at 3,812,886 to 3,812,972
_R0122
Shewana3_R0123: Shewana3_R0123 - tRNA-Leu (RefSeq), at 3,813,017 to 3,813,103
_R0123
Shewana3_3173: Shewana3_3173 - methyl-accepting chemotaxis sensory transducer (RefSeq), at 3,813,267 to 3,814,892
_3173
Position (kb)
3812
3813 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3812.915 kb on + strand, within Shewana3_R0122 at 3812.948 kb on + strand, within Shewana3_R0122 at 3812.957 kb on + strand, within Shewana3_R0122 at 3813.163 kb on + strand at 3813.220 kb on + strand at 3813.238 kb on - strand at 3813.238 kb on - strand at 3813.719 kb on + strand, within Shewana3_3173 at 3813.879 kb on - strand, within Shewana3_3173 at 3813.935 kb on + strand, within Shewana3_3173 at 3813.955 kb on + strand, within Shewana3_3173
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,812,915 + Shewana3_R0122 0.33 -1.8 3,812,948 + Shewana3_R0122 0.71 -4.1 3,812,957 + Shewana3_R0122 0.82 -2.3 3,813,163 + -0.5 3,813,220 + +0.8 3,813,238 - +0.6 3,813,238 - +0.3 3,813,719 + Shewana3_3173 0.28 +1.2 3,813,879 - Shewana3_3173 0.38 +0.8 3,813,935 + Shewana3_3173 0.41 -1.8 3,813,955 + Shewana3_3173 0.42 +0.4
Or see this region's nucleotide sequence