Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_2703 and Shewana3_2704 are separated by 47 nucleotides Shewana3_2704 and Shewana3_2705 are separated by 109 nucleotides Shewana3_2705 and Shewana3_2706 are separated by 171 nucleotides Shewana3_2706 and Shewana3_2707 are separated by 152 nucleotides
Shewana3_2703: Shewana3_2703 - TonB-dependent receptor (RefSeq), at 3,230,269 to 3,233,124
_2703
Shewana3_2704: Shewana3_2704 - helix-turn-helix, type 11 domain-containing protein (RefSeq), at 3,233,172 to 3,233,870
_2704
Shewana3_2705: Shewana3_2705 - transcription activator, effector binding (RefSeq), at 3,233,980 to 3,234,429
_2705
Shewana3_2706: Shewana3_2706 - glyoxalase/bleomycin resistance protein/dioxygenase (RefSeq), at 3,234,601 to 3,234,984
_2706
Shewana3_2707: Shewana3_2707 - phosphoglucomutase/phosphomannomutase alpha/beta/subunit (RefSeq), at 3,235,137 to 3,236,858
_2707
Position (kb)
3233
3234
3235 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3233.020 kb on - strand at 3233.065 kb on + strand at 3233.765 kb on - strand, within Shewana3_2704 at 3233.798 kb on + strand, within Shewana3_2704 at 3234.072 kb on + strand, within Shewana3_2705 at 3234.190 kb on - strand, within Shewana3_2705 at 3234.274 kb on - strand, within Shewana3_2705 at 3234.426 kb on + strand at 3234.765 kb on + strand, within Shewana3_2706 at 3234.853 kb on - strand, within Shewana3_2706 at 3235.068 kb on - strand at 3235.200 kb on - strand at 3235.410 kb on - strand, within Shewana3_2707
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 3,233,020 - -0.2 3,233,065 + -0.0 3,233,765 - Shewana3_2704 0.85 +0.5 3,233,798 + Shewana3_2704 0.90 +0.4 3,234,072 + Shewana3_2705 0.20 +0.5 3,234,190 - Shewana3_2705 0.47 -1.3 3,234,274 - Shewana3_2705 0.65 -1.8 3,234,426 + -0.6 3,234,765 + Shewana3_2706 0.43 -0.3 3,234,853 - Shewana3_2706 0.66 -2.1 3,235,068 - -3.2 3,235,200 - +0.1 3,235,410 - Shewana3_2707 0.16 +0.2
Or see this region's nucleotide sequence