Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0086 and Shewana3_0087 are separated by 71 nucleotides Shewana3_0087 and Shewana3_0088 are separated by 15 nucleotides Shewana3_0088 and Shewana3_0089 are separated by 62 nucleotides
Shewana3_0086: Shewana3_0086 - MerR family transcriptional regulator (RefSeq), at 99,057 to 99,410
_0086
Shewana3_0087: Shewana3_0087 - NAD(P)H dehydrogenase (quinone) (RefSeq), at 99,482 to 100,060
_0087
Shewana3_0088: Shewana3_0088 - glutathione-dependent formaldehyde-activating, GFA (RefSeq), at 100,076 to 100,471
_0088
Shewana3_0089: Shewana3_0089 - 2,3-diketo-5-methylthio-1-phosphopentane phosphatase (RefSeq), at 100,534 to 101,214
_0089
Position (kb) 100
101 Strain fitness (log2 ratio) -2
-1
0
1 at 99.126 kb on + strand, within Shewana3_0086 at 99.189 kb on - strand, within Shewana3_0086 at 99.267 kb on - strand, within Shewana3_0086 at 99.288 kb on + strand, within Shewana3_0086 at 99.449 kb on - strand at 99.449 kb on - strand at 99.751 kb on + strand, within Shewana3_0087 at 99.817 kb on - strand, within Shewana3_0087 at 100.097 kb on + strand at 100.153 kb on - strand, within Shewana3_0088 at 100.153 kb on - strand, within Shewana3_0088 at 100.170 kb on - strand, within Shewana3_0088 at 100.219 kb on + strand, within Shewana3_0088 at 100.525 kb on + strand at 100.605 kb on + strand, within Shewana3_0089 at 100.605 kb on + strand, within Shewana3_0089 at 100.640 kb on + strand, within Shewana3_0089 at 100.640 kb on + strand, within Shewana3_0089 at 100.694 kb on - strand, within Shewana3_0089 at 100.728 kb on - strand, within Shewana3_0089 at 100.827 kb on - strand, within Shewana3_0089 at 100.903 kb on + strand, within Shewana3_0089 at 100.903 kb on + strand, within Shewana3_0089 at 100.935 kb on - strand, within Shewana3_0089 at 100.969 kb on - strand, within Shewana3_0089 at 101.080 kb on + strand, within Shewana3_0089 at 101.122 kb on + strand, within Shewana3_0089 at 101.122 kb on + strand, within Shewana3_0089 at 101.169 kb on - strand at 101.188 kb on + strand at 101.196 kb on - strand at 101.268 kb on + strand at 101.325 kb on + strand at 101.388 kb on - strand at 101.420 kb on + strand at 101.428 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 99,126 + Shewana3_0086 0.19 +0.4 99,189 - Shewana3_0086 0.37 +1.3 99,267 - Shewana3_0086 0.59 -1.1 99,288 + Shewana3_0086 0.65 -2.0 99,449 - -0.2 99,449 - -1.6 99,751 + Shewana3_0087 0.46 -1.6 99,817 - Shewana3_0087 0.58 -0.1 100,097 + -2.7 100,153 - Shewana3_0088 0.19 +0.4 100,153 - Shewana3_0088 0.19 -0.3 100,170 - Shewana3_0088 0.24 -1.0 100,219 + Shewana3_0088 0.36 -0.1 100,525 + +0.3 100,605 + Shewana3_0089 0.10 +0.4 100,605 + Shewana3_0089 0.10 +0.6 100,640 + Shewana3_0089 0.16 +0.6 100,640 + Shewana3_0089 0.16 +0.7 100,694 - Shewana3_0089 0.23 -0.4 100,728 - Shewana3_0089 0.28 +0.1 100,827 - Shewana3_0089 0.43 -2.3 100,903 + Shewana3_0089 0.54 -0.1 100,903 + Shewana3_0089 0.54 -1.3 100,935 - Shewana3_0089 0.59 +0.2 100,969 - Shewana3_0089 0.64 +0.3 101,080 + Shewana3_0089 0.80 -1.6 101,122 + Shewana3_0089 0.86 +0.3 101,122 + Shewana3_0089 0.86 +1.1 101,169 - +0.5 101,188 + -1.2 101,196 - +1.0 101,268 + +0.1 101,325 + -0.2 101,388 - -1.0 101,420 + +0.1 101,428 - -1.8
Or see this region's nucleotide sequence