Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_0080 and Shewana3_0081 are separated by 140 nucleotides Shewana3_0081 and Shewana3_0082 are separated by 107 nucleotides Shewana3_0082 and Shewana3_0083 are separated by 118 nucleotides
Shewana3_0080: Shewana3_0080 - AMP-dependent synthetase and ligase (RefSeq), at 92,480 to 94,273
_0080
Shewana3_0081: Shewana3_0081 - MORN repeat-containing protein (RefSeq), at 94,414 to 96,903
_0081
Shewana3_0082: Shewana3_0082 - thioesterase superfamily protein (RefSeq), at 97,011 to 97,421
_0082
Shewana3_0083: Shewana3_0083 - HPP family protein (RefSeq), at 97,540 to 98,088
_0083
Position (kb) 94
95
96
97 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 93.769 kb on + strand, within Shewana3_0080 at 93.973 kb on + strand, within Shewana3_0080 at 94.027 kb on + strand, within Shewana3_0080 at 94.110 kb on + strand at 94.123 kb on - strand at 94.157 kb on - strand at 94.171 kb on - strand at 94.391 kb on + strand at 94.398 kb on - strand at 94.459 kb on - strand at 94.464 kb on - strand at 94.682 kb on + strand, within Shewana3_0081 at 94.682 kb on + strand, within Shewana3_0081 at 94.700 kb on - strand, within Shewana3_0081 at 94.741 kb on + strand, within Shewana3_0081 at 94.817 kb on + strand, within Shewana3_0081 at 94.851 kb on - strand, within Shewana3_0081 at 94.887 kb on + strand, within Shewana3_0081 at 95.047 kb on - strand, within Shewana3_0081 at 95.095 kb on - strand, within Shewana3_0081 at 95.113 kb on + strand, within Shewana3_0081 at 95.166 kb on - strand, within Shewana3_0081 at 95.234 kb on + strand, within Shewana3_0081 at 95.237 kb on - strand, within Shewana3_0081 at 95.282 kb on - strand, within Shewana3_0081 at 95.348 kb on - strand, within Shewana3_0081 at 95.349 kb on + strand, within Shewana3_0081 at 95.385 kb on + strand, within Shewana3_0081 at 95.420 kb on + strand, within Shewana3_0081 at 95.528 kb on - strand, within Shewana3_0081 at 95.782 kb on + strand, within Shewana3_0081 at 95.847 kb on + strand, within Shewana3_0081 at 95.894 kb on + strand, within Shewana3_0081 at 96.046 kb on - strand, within Shewana3_0081 at 96.056 kb on - strand, within Shewana3_0081 at 96.080 kb on - strand, within Shewana3_0081 at 96.095 kb on - strand, within Shewana3_0081 at 96.188 kb on + strand, within Shewana3_0081 at 96.214 kb on + strand, within Shewana3_0081 at 96.434 kb on - strand, within Shewana3_0081 at 96.434 kb on - strand, within Shewana3_0081 at 96.492 kb on + strand, within Shewana3_0081 at 96.586 kb on + strand, within Shewana3_0081 at 96.676 kb on - strand at 96.836 kb on - strand at 96.836 kb on - strand at 96.975 kb on + strand at 96.983 kb on - strand at 97.065 kb on + strand, within Shewana3_0082 at 97.111 kb on - strand, within Shewana3_0082 at 97.267 kb on + strand, within Shewana3_0082 at 97.341 kb on + strand, within Shewana3_0082 at 97.346 kb on - strand, within Shewana3_0082 at 97.392 kb on - strand at 97.393 kb on + strand at 97.406 kb on + strand at 97.424 kb on - strand at 97.575 kb on + strand at 97.575 kb on + strand at 97.688 kb on + strand, within Shewana3_0083 at 97.726 kb on + strand, within Shewana3_0083 at 97.726 kb on + strand, within Shewana3_0083 at 97.734 kb on - strand, within Shewana3_0083 at 97.789 kb on + strand, within Shewana3_0083
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 93,769 + Shewana3_0080 0.72 -1.7 93,973 + Shewana3_0080 0.83 -0.1 94,027 + Shewana3_0080 0.86 +0.2 94,110 + -0.2 94,123 - +0.3 94,157 - +0.3 94,171 - +0.2 94,391 + -0.1 94,398 - -1.8 94,459 - -1.5 94,464 - +0.9 94,682 + Shewana3_0081 0.11 -1.6 94,682 + Shewana3_0081 0.11 -1.8 94,700 - Shewana3_0081 0.11 -0.1 94,741 + Shewana3_0081 0.13 +0.5 94,817 + Shewana3_0081 0.16 -2.3 94,851 - Shewana3_0081 0.18 -1.2 94,887 + Shewana3_0081 0.19 -2.3 95,047 - Shewana3_0081 0.25 -1.6 95,095 - Shewana3_0081 0.27 -2.4 95,113 + Shewana3_0081 0.28 -2.7 95,166 - Shewana3_0081 0.30 -2.3 95,234 + Shewana3_0081 0.33 -2.0 95,237 - Shewana3_0081 0.33 -1.6 95,282 - Shewana3_0081 0.35 +0.4 95,348 - Shewana3_0081 0.38 +0.2 95,349 + Shewana3_0081 0.38 -1.5 95,385 + Shewana3_0081 0.39 -2.1 95,420 + Shewana3_0081 0.40 +0.3 95,528 - Shewana3_0081 0.45 +1.0 95,782 + Shewana3_0081 0.55 -3.3 95,847 + Shewana3_0081 0.58 -0.2 95,894 + Shewana3_0081 0.59 +0.8 96,046 - Shewana3_0081 0.66 +0.1 96,056 - Shewana3_0081 0.66 -1.7 96,080 - Shewana3_0081 0.67 -0.2 96,095 - Shewana3_0081 0.68 -1.4 96,188 + Shewana3_0081 0.71 +0.6 96,214 + Shewana3_0081 0.72 +0.0 96,434 - Shewana3_0081 0.81 +0.7 96,434 - Shewana3_0081 0.81 -0.5 96,492 + Shewana3_0081 0.83 -2.7 96,586 + Shewana3_0081 0.87 +2.3 96,676 - -1.8 96,836 - +0.2 96,836 - +0.6 96,975 + -0.0 96,983 - -2.6 97,065 + Shewana3_0082 0.13 -1.3 97,111 - Shewana3_0082 0.24 -1.1 97,267 + Shewana3_0082 0.62 -2.9 97,341 + Shewana3_0082 0.80 -3.1 97,346 - Shewana3_0082 0.82 -0.9 97,392 - -1.1 97,393 + -0.2 97,406 + +0.2 97,424 - +0.1 97,575 + +0.1 97,575 + +0.3 97,688 + Shewana3_0083 0.27 -1.0 97,726 + Shewana3_0083 0.34 -1.6 97,726 + Shewana3_0083 0.34 -2.3 97,734 - Shewana3_0083 0.35 +0.0 97,789 + Shewana3_0083 0.45 +0.1
Or see this region's nucleotide sequence