Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4297 and Shewana3_4298 are separated by 28 nucleotides Shewana3_4298 and Shewana3_4299 are separated by 113 nucleotides
Shewana3_4297: Shewana3_4297 - copper/silver efflux system outer membrane protein CusC (RefSeq), at 180,422 to 181,807
_4297
Shewana3_4298: Shewana3_4298 - periplasmic copper-binding protein (RefSeq), at 181,836 to 182,189
_4298
Shewana3_4299: Shewana3_4299 - copper/silver efflux system membrane fusion protein CusB (RefSeq), at 182,303 to 183,595
_4299
Position (kb) 181
182
183 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 180.905 kb on + strand, within Shewana3_4297 at 181.009 kb on + strand, within Shewana3_4297 at 181.017 kb on - strand, within Shewana3_4297 at 181.017 kb on - strand, within Shewana3_4297 at 181.062 kb on + strand, within Shewana3_4297 at 181.062 kb on + strand, within Shewana3_4297 at 181.070 kb on - strand, within Shewana3_4297 at 181.352 kb on - strand, within Shewana3_4297 at 181.352 kb on - strand, within Shewana3_4297 at 181.468 kb on - strand, within Shewana3_4297 at 181.545 kb on + strand, within Shewana3_4297 at 181.563 kb on + strand, within Shewana3_4297 at 181.575 kb on + strand, within Shewana3_4297 at 181.689 kb on - strand at 181.746 kb on + strand at 181.754 kb on - strand at 181.855 kb on - strand at 182.144 kb on + strand, within Shewana3_4298 at 182.178 kb on + strand at 182.196 kb on + strand at 182.420 kb on + strand at 182.513 kb on + strand, within Shewana3_4299 at 182.565 kb on - strand, within Shewana3_4299 at 182.580 kb on + strand, within Shewana3_4299 at 182.800 kb on + strand, within Shewana3_4299 at 182.877 kb on + strand, within Shewana3_4299 at 183.181 kb on - strand, within Shewana3_4299
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 180,905 + Shewana3_4297 0.35 -0.0 181,009 + Shewana3_4297 0.42 +0.5 181,017 - Shewana3_4297 0.43 -2.1 181,017 - Shewana3_4297 0.43 -2.2 181,062 + Shewana3_4297 0.46 -0.0 181,062 + Shewana3_4297 0.46 +0.0 181,070 - Shewana3_4297 0.47 -2.2 181,352 - Shewana3_4297 0.67 +0.4 181,352 - Shewana3_4297 0.67 +0.1 181,468 - Shewana3_4297 0.75 +0.7 181,545 + Shewana3_4297 0.81 -0.8 181,563 + Shewana3_4297 0.82 -0.5 181,575 + Shewana3_4297 0.83 +0.4 181,689 - +0.3 181,746 + -0.5 181,754 - +1.5 181,855 - +0.1 182,144 + Shewana3_4298 0.87 +0.5 182,178 + -1.1 182,196 + -2.0 182,420 + -1.4 182,513 + Shewana3_4299 0.16 -4.6 182,565 - Shewana3_4299 0.20 +0.1 182,580 + Shewana3_4299 0.21 -0.6 182,800 + Shewana3_4299 0.38 +0.6 182,877 + Shewana3_4299 0.44 -1.6 183,181 - Shewana3_4299 0.68 +0.1
Or see this region's nucleotide sequence