Experiment: L-Isoleucine (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shewana3_4218 and Shewana3_4219 are separated by 12 nucleotides Shewana3_4219 and Shewana3_4220 overlap by 1 nucleotides
Shewana3_4218: Shewana3_4218 - Type IV secretory pathway VirB4 components-like protein (RefSeq), at 89,025 to 91,709
_4218
Shewana3_4219: Shewana3_4219 - hypothetical protein (RefSeq), at 91,722 to 92,450
_4219
Shewana3_4220: Shewana3_4220 - hypothetical protein (RefSeq), at 92,450 to 93,277
_4220
Position (kb) 91
92
93 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 90.741 kb on + strand, within Shewana3_4218 at 90.748 kb on - strand, within Shewana3_4218 at 91.030 kb on + strand, within Shewana3_4218 at 91.190 kb on + strand, within Shewana3_4218 at 91.295 kb on + strand, within Shewana3_4218 at 91.322 kb on + strand, within Shewana3_4218 at 91.434 kb on + strand, within Shewana3_4218 at 91.434 kb on + strand, within Shewana3_4218 at 91.452 kb on - strand at 91.489 kb on - strand at 91.558 kb on - strand at 91.870 kb on - strand, within Shewana3_4219 at 92.080 kb on - strand, within Shewana3_4219 at 92.080 kb on - strand, within Shewana3_4219 at 92.140 kb on - strand, within Shewana3_4219 at 92.212 kb on + strand, within Shewana3_4219 at 92.330 kb on - strand, within Shewana3_4219 at 92.334 kb on + strand, within Shewana3_4219 at 92.571 kb on - strand, within Shewana3_4220 at 92.605 kb on - strand, within Shewana3_4220 at 92.789 kb on - strand, within Shewana3_4220 at 92.872 kb on - strand, within Shewana3_4220 at 92.887 kb on - strand, within Shewana3_4220 at 92.913 kb on + strand, within Shewana3_4220 at 93.003 kb on - strand, within Shewana3_4220 at 93.147 kb on + strand, within Shewana3_4220
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (C) remove 90,741 + Shewana3_4218 0.64 +0.0 90,748 - Shewana3_4218 0.64 +0.3 91,030 + Shewana3_4218 0.75 +1.1 91,190 + Shewana3_4218 0.81 -2.4 91,295 + Shewana3_4218 0.85 +0.2 91,322 + Shewana3_4218 0.86 +0.0 91,434 + Shewana3_4218 0.90 +0.3 91,434 + Shewana3_4218 0.90 -0.3 91,452 - +0.2 91,489 - -0.1 91,558 - -3.2 91,870 - Shewana3_4219 0.20 +0.3 92,080 - Shewana3_4219 0.49 +1.6 92,080 - Shewana3_4219 0.49 +0.7 92,140 - Shewana3_4219 0.57 +0.7 92,212 + Shewana3_4219 0.67 +0.7 92,330 - Shewana3_4219 0.83 +0.4 92,334 + Shewana3_4219 0.84 -3.1 92,571 - Shewana3_4220 0.15 -2.3 92,605 - Shewana3_4220 0.19 -3.2 92,789 - Shewana3_4220 0.41 -0.7 92,872 - Shewana3_4220 0.51 -2.1 92,887 - Shewana3_4220 0.53 +0.2 92,913 + Shewana3_4220 0.56 +0.2 93,003 - Shewana3_4220 0.67 +0.5 93,147 + Shewana3_4220 0.84 -0.8
Or see this region's nucleotide sequence