Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2573 and Shew_2574 are separated by 2 nucleotides Shew_2574 and Shew_2575 are separated by 46 nucleotides Shew_2575 and Shew_2576 are separated by 12 nucleotides
Shew_2573: Shew_2573 - carbamoyl-phosphate synthase L chain, ATP-binding (RefSeq), at 3,032,237 to 3,034,270
_2573
Shew_2574: Shew_2574 - pyruvate carboxyltransferase (RefSeq), at 3,034,273 to 3,035,178
_2574
Shew_2575: Shew_2575 - 3-oxoacid CoA-transferase, A subunit (RefSeq), at 3,035,225 to 3,035,926
_2575
Shew_2576: Shew_2576 - 3-oxoacid CoA-transferase, B subunit (RefSeq), at 3,035,939 to 3,036,595
_2576
Position (kb)
3034
3035
3036 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5
6 at 3033.542 kb on + strand, within Shew_2573 at 3033.758 kb on - strand, within Shew_2573 at 3033.822 kb on + strand, within Shew_2573 at 3034.033 kb on + strand, within Shew_2573 at 3034.124 kb on + strand at 3034.609 kb on - strand, within Shew_2574 at 3035.162 kb on - strand at 3036.139 kb on - strand, within Shew_2576
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 3,033,542 + Shew_2573 0.64 +2.7 3,033,758 - Shew_2573 0.75 +2.9 3,033,822 + Shew_2573 0.78 +1.7 3,034,033 + Shew_2573 0.88 +2.1 3,034,124 + +2.1 3,034,609 - Shew_2574 0.37 +6.5 3,035,162 - +0.8 3,036,139 - Shew_2576 0.30 +0.8
Or see this region's nucleotide sequence