Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_2323 and Shew_2324 are separated by 316 nucleotides Shew_2324 and Shew_2325 are separated by 113 nucleotides Shew_2325 and Shew_2326 overlap by 14 nucleotides Shew_2326 and Shew_2327 are separated by 32 nucleotides Shew_2327 and Shew_2328 are separated by 196 nucleotides
Shew_2323: Shew_2323 - phosphate-starvation-inducible E (RefSeq), at 2,718,642 to 2,719,046
_2323
Shew_2324: Shew_2324 - type IV pilus assembly PilZ (RefSeq), at 2,719,363 to 2,719,665
_2324
Shew_2325: Shew_2325 - hypothetical protein (RefSeq), at 2,719,779 to 2,720,447
_2325
Shew_2326: Shew_2326 - RepA domain-containing protein (RefSeq), at 2,720,434 to 2,720,778
_2326
Shew_2327: Shew_2327 - hypothetical protein (RefSeq), at 2,720,811 to 2,721,014
_2327
Shew_2328: Shew_2328 - signal transduction histidine kinase, LytS (RefSeq), at 2,721,211 to 2,722,908
_2328
Position (kb)
2719
2720
2721 Strain fitness (log2 ratio)
-1
0
1
2 at 2718.786 kb on + strand, within Shew_2323 at 2719.183 kb on - strand at 2719.464 kb on + strand, within Shew_2324 at 2719.570 kb on - strand, within Shew_2324 at 2719.909 kb on + strand, within Shew_2325 at 2719.909 kb on - strand, within Shew_2325 at 2720.115 kb on - strand, within Shew_2325 at 2720.171 kb on + strand, within Shew_2325 at 2720.283 kb on - strand, within Shew_2325 at 2720.283 kb on - strand, within Shew_2325 at 2720.867 kb on + strand, within Shew_2327
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 2,718,786 + Shew_2323 0.36 -0.7 2,719,183 - -0.7 2,719,464 + Shew_2324 0.33 +0.3 2,719,570 - Shew_2324 0.68 -0.2 2,719,909 + Shew_2325 0.19 +0.6 2,719,909 - Shew_2325 0.19 +1.1 2,720,115 - Shew_2325 0.50 -0.1 2,720,171 + Shew_2325 0.59 -1.1 2,720,283 - Shew_2325 0.75 +1.1 2,720,283 - Shew_2325 0.75 +0.3 2,720,867 + Shew_2327 0.27 +2.3
Or see this region's nucleotide sequence