Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Shew_1668 and Shew_1669 are separated by 162 nucleotides Shew_1669 and Shew_1670 are separated by 15 nucleotides Shew_1670 and Shew_1671 overlap by 1 nucleotides
Shew_1668: Shew_1668 - methylmalonate-semialdehyde dehydrogenase (RefSeq), at 1,917,266 to 1,918,771
_1668
Shew_1669: Shew_1669 - butyryl-CoA dehydrogenase (RefSeq), at 1,918,934 to 1,920,091
_1669
Shew_1670: Shew_1670 - enoyl-CoA hydratase (RefSeq), at 1,920,107 to 1,920,880
_1670
Shew_1671: Shew_1671 - enoyl-CoA hydratase/isomerase (RefSeq), at 1,920,880 to 1,922,010
_1671
Position (kb)
1918
1919
1920
1921 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1918.118 kb on - strand, within Shew_1668 at 1918.279 kb on + strand, within Shew_1668 at 1918.287 kb on - strand, within Shew_1668 at 1918.287 kb on - strand, within Shew_1668 at 1919.184 kb on + strand, within Shew_1669 at 1919.291 kb on + strand, within Shew_1669 at 1919.299 kb on - strand, within Shew_1669 at 1919.352 kb on - strand, within Shew_1669 at 1919.371 kb on - strand, within Shew_1669 at 1919.681 kb on - strand, within Shew_1669 at 1919.720 kb on - strand, within Shew_1669 at 1920.249 kb on + strand, within Shew_1670 at 1920.249 kb on + strand, within Shew_1670 at 1920.533 kb on + strand, within Shew_1670 at 1920.541 kb on - strand, within Shew_1670 at 1920.627 kb on - strand, within Shew_1670 at 1920.876 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 1,918,118 - Shew_1668 0.57 +1.5 1,918,279 + Shew_1668 0.67 +1.4 1,918,287 - Shew_1668 0.68 +1.4 1,918,287 - Shew_1668 0.68 +1.4 1,919,184 + Shew_1669 0.22 +1.2 1,919,291 + Shew_1669 0.31 +0.4 1,919,299 - Shew_1669 0.32 +1.2 1,919,352 - Shew_1669 0.36 +0.1 1,919,371 - Shew_1669 0.38 +2.4 1,919,681 - Shew_1669 0.65 +1.6 1,919,720 - Shew_1669 0.68 +2.4 1,920,249 + Shew_1670 0.18 +1.0 1,920,249 + Shew_1670 0.18 +0.7 1,920,533 + Shew_1670 0.55 -0.2 1,920,541 - Shew_1670 0.56 -1.9 1,920,627 - Shew_1670 0.67 -2.4 1,920,876 + +0.2
Or see this region's nucleotide sequence