Experiment: D-Fructose (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_678 and Pf6N2E2_679 overlap by 4 nucleotides Pf6N2E2_679 and Pf6N2E2_680 are separated by 191 nucleotides Pf6N2E2_680 and Pf6N2E2_681 overlap by 4 nucleotides
Pf6N2E2_678 - Putative oxidoreductase in 4-hydroxyproline catabolic gene cluster, at 783,039 to 784,295
_678
Pf6N2E2_679 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 784,292 to 784,528
_679
Pf6N2E2_680 - D-amino-acid oxidase (EC 1.4.3.3), at 784,720 to 785,835
_680
Pf6N2E2_681 - 4-hydroxyproline epimerase (EC 5.1.1.8), at 785,832 to 786,764
_681
Position (kb)
784
785
786 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 784.000 kb on + strand, within Pf6N2E2_678 at 784.001 kb on - strand, within Pf6N2E2_678 at 784.047 kb on - strand, within Pf6N2E2_678 at 784.089 kb on + strand, within Pf6N2E2_678 at 784.089 kb on + strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.090 kb on - strand, within Pf6N2E2_678 at 784.387 kb on + strand, within Pf6N2E2_679 at 784.387 kb on + strand, within Pf6N2E2_679 at 784.517 kb on + strand at 784.518 kb on - strand at 784.760 kb on + strand at 784.760 kb on + strand at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.092 kb on + strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.093 kb on - strand, within Pf6N2E2_680 at 785.094 kb on + strand, within Pf6N2E2_680 at 785.094 kb on + strand, within Pf6N2E2_680 at 785.095 kb on - strand, within Pf6N2E2_680 at 785.095 kb on - strand, within Pf6N2E2_680 at 785.142 kb on - strand, within Pf6N2E2_680 at 785.142 kb on - strand, within Pf6N2E2_680 at 785.397 kb on - strand, within Pf6N2E2_680 at 785.522 kb on + strand, within Pf6N2E2_680 at 785.522 kb on + strand, within Pf6N2E2_680 at 785.523 kb on - strand, within Pf6N2E2_680 at 785.947 kb on + strand, within Pf6N2E2_681 at 785.947 kb on + strand, within Pf6N2E2_681 at 786.073 kb on + strand, within Pf6N2E2_681 at 786.074 kb on - strand, within Pf6N2E2_681 at 786.093 kb on + strand, within Pf6N2E2_681 at 786.093 kb on + strand, within Pf6N2E2_681 at 786.198 kb on + strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.206 kb on - strand, within Pf6N2E2_681 at 786.211 kb on + strand, within Pf6N2E2_681 at 786.355 kb on + strand, within Pf6N2E2_681 at 786.355 kb on + strand, within Pf6N2E2_681 at 786.408 kb on + strand, within Pf6N2E2_681 at 786.832 kb on - strand at 786.832 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction D-Fructose (C) remove 784,000 + Pf6N2E2_678 0.76 -1.9 784,001 - Pf6N2E2_678 0.77 +0.6 784,047 - Pf6N2E2_678 0.80 -1.3 784,089 + Pf6N2E2_678 0.84 +2.8 784,089 + Pf6N2E2_678 0.84 -1.4 784,090 - Pf6N2E2_678 0.84 -0.1 784,090 - Pf6N2E2_678 0.84 +0.2 784,090 - Pf6N2E2_678 0.84 -0.1 784,090 - Pf6N2E2_678 0.84 -0.1 784,387 + Pf6N2E2_679 0.40 -1.5 784,387 + Pf6N2E2_679 0.40 +1.0 784,517 + -1.1 784,518 - -0.0 784,760 + +1.1 784,760 + -0.1 785,092 + Pf6N2E2_680 0.33 -0.1 785,092 + Pf6N2E2_680 0.33 -1.4 785,092 + Pf6N2E2_680 0.33 +1.7 785,092 + Pf6N2E2_680 0.33 -1.2 785,092 + Pf6N2E2_680 0.33 +0.5 785,093 - Pf6N2E2_680 0.33 -0.6 785,093 - Pf6N2E2_680 0.33 +1.1 785,093 - Pf6N2E2_680 0.33 +0.8 785,093 - Pf6N2E2_680 0.33 +0.8 785,093 - Pf6N2E2_680 0.33 +0.3 785,093 - Pf6N2E2_680 0.33 -0.1 785,093 - Pf6N2E2_680 0.33 +1.8 785,094 + Pf6N2E2_680 0.34 +1.3 785,094 + Pf6N2E2_680 0.34 +0.6 785,095 - Pf6N2E2_680 0.34 -0.1 785,095 - Pf6N2E2_680 0.34 +0.5 785,142 - Pf6N2E2_680 0.38 +0.7 785,142 - Pf6N2E2_680 0.38 +1.3 785,397 - Pf6N2E2_680 0.61 -1.2 785,522 + Pf6N2E2_680 0.72 +1.2 785,522 + Pf6N2E2_680 0.72 +0.0 785,523 - Pf6N2E2_680 0.72 -0.4 785,947 + Pf6N2E2_681 0.12 -0.4 785,947 + Pf6N2E2_681 0.12 +0.6 786,073 + Pf6N2E2_681 0.26 +0.3 786,074 - Pf6N2E2_681 0.26 +0.6 786,093 + Pf6N2E2_681 0.28 -1.4 786,093 + Pf6N2E2_681 0.28 -1.3 786,198 + Pf6N2E2_681 0.39 -1.3 786,206 - Pf6N2E2_681 0.40 -0.1 786,206 - Pf6N2E2_681 0.40 -0.5 786,206 - Pf6N2E2_681 0.40 -0.3 786,211 + Pf6N2E2_681 0.41 +0.2 786,355 + Pf6N2E2_681 0.56 +0.5 786,355 + Pf6N2E2_681 0.56 +0.5 786,408 + Pf6N2E2_681 0.62 +0.1 786,832 - +0.5 786,832 - -0.9
Or see this region's nucleotide sequence