Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS24725 and CA265_RS24730 are separated by 226 nucleotides CA265_RS24730 and CA265_RS24735 are separated by 70 nucleotides CA265_RS24735 and CA265_RS24740 are separated by 224 nucleotides
CA265_RS24725: CA265_RS24725 - multidrug transporter, at 6,037,484 to 6,040,510
_RS24725
CA265_RS24730: CA265_RS24730 - tRNA-Gln, at 6,040,737 to 6,040,810
_RS24730
CA265_RS24735: CA265_RS24735 - ribose-phosphate pyrophosphokinase, at 6,040,881 to 6,041,822
_RS24735
CA265_RS24740: CA265_RS24740 - 50S ribosomal protein L25/general stress protein Ctc, at 6,042,047 to 6,042,631
_RS24740
Position (kb)
6040
6041
6042 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 6039.905 kb on + strand, within CA265_RS24725 at 6039.922 kb on + strand, within CA265_RS24725 at 6039.967 kb on - strand, within CA265_RS24725 at 6039.967 kb on - strand, within CA265_RS24725 at 6040.041 kb on - strand, within CA265_RS24725 at 6040.072 kb on - strand, within CA265_RS24725 at 6040.085 kb on + strand, within CA265_RS24725 at 6040.086 kb on - strand, within CA265_RS24725 at 6040.125 kb on - strand, within CA265_RS24725 at 6040.125 kb on - strand, within CA265_RS24725 at 6040.219 kb on + strand at 6040.274 kb on - strand at 6040.284 kb on + strand at 6040.289 kb on + strand at 6040.503 kb on + strand at 6040.522 kb on - strand at 6041.820 kb on + strand at 6041.820 kb on + strand at 6041.852 kb on + strand at 6041.874 kb on + strand at 6041.874 kb on + strand at 6041.894 kb on + strand at 6041.944 kb on + strand at 6041.950 kb on + strand at 6042.697 kb on + strand at 6042.768 kb on - strand at 6042.777 kb on + strand at 6042.779 kb on + strand at 6042.780 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 6,039,905 + CA265_RS24725 0.80 -1.2 6,039,922 + CA265_RS24725 0.81 -0.3 6,039,967 - CA265_RS24725 0.82 +0.2 6,039,967 - CA265_RS24725 0.82 +0.5 6,040,041 - CA265_RS24725 0.84 +0.5 6,040,072 - CA265_RS24725 0.85 +0.3 6,040,085 + CA265_RS24725 0.86 +0.7 6,040,086 - CA265_RS24725 0.86 +0.3 6,040,125 - CA265_RS24725 0.87 -0.0 6,040,125 - CA265_RS24725 0.87 -0.7 6,040,219 + -1.5 6,040,274 - -0.9 6,040,284 + +1.1 6,040,289 + -0.5 6,040,503 + +0.4 6,040,522 - -0.2 6,041,820 + -1.1 6,041,820 + -0.6 6,041,852 + -0.9 6,041,874 + -0.1 6,041,874 + -0.6 6,041,894 + -3.1 6,041,944 + -0.8 6,041,950 + -0.3 6,042,697 + -0.5 6,042,768 - +1.9 6,042,777 + -0.6 6,042,779 + -0.2 6,042,780 - -0.5
Or see this region's nucleotide sequence