Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS24160 and CA265_RS24165 overlap by 4 nucleotides CA265_RS24165 and CA265_RS24170 are separated by 110 nucleotides
CA265_RS24160: CA265_RS24160 - bifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase, at 5,885,360 to 5,886,178
_RS24160
CA265_RS24165: CA265_RS24165 - thiaminase II, at 5,886,175 to 5,886,828
_RS24165
CA265_RS24170: CA265_RS24170 - MFS transporter, at 5,886,939 to 5,888,144
_RS24170
Position (kb)
5886
5887 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5885.209 kb on + strand at 5885.224 kb on - strand at 5885.239 kb on + strand at 5885.278 kb on - strand at 5885.334 kb on + strand at 5885.366 kb on + strand at 5885.367 kb on - strand at 5885.376 kb on + strand at 5885.376 kb on + strand at 5885.377 kb on - strand at 5885.377 kb on - strand at 5885.377 kb on - strand at 5885.473 kb on + strand, within CA265_RS24160 at 5885.474 kb on - strand, within CA265_RS24160 at 5885.551 kb on - strand at 5885.570 kb on - strand, within CA265_RS24160 at 5885.572 kb on - strand, within CA265_RS24160 at 5885.572 kb on - strand, within CA265_RS24160 at 5885.572 kb on - strand, within CA265_RS24160 at 5885.572 kb on - strand, within CA265_RS24160 at 5885.657 kb on + strand, within CA265_RS24160 at 5885.771 kb on - strand, within CA265_RS24160 at 5885.916 kb on - strand, within CA265_RS24160 at 5885.969 kb on - strand, within CA265_RS24160 at 5886.001 kb on + strand, within CA265_RS24160 at 5886.002 kb on - strand, within CA265_RS24160 at 5886.165 kb on + strand at 5886.169 kb on + strand at 5886.274 kb on - strand, within CA265_RS24165 at 5886.311 kb on - strand, within CA265_RS24165 at 5886.338 kb on + strand, within CA265_RS24165 at 5886.349 kb on - strand, within CA265_RS24165 at 5886.447 kb on - strand, within CA265_RS24165 at 5886.459 kb on - strand, within CA265_RS24165 at 5886.497 kb on - strand, within CA265_RS24165 at 5886.505 kb on + strand, within CA265_RS24165 at 5886.506 kb on - strand, within CA265_RS24165 at 5886.507 kb on + strand, within CA265_RS24165 at 5886.536 kb on + strand, within CA265_RS24165 at 5886.579 kb on + strand, within CA265_RS24165 at 5886.580 kb on - strand, within CA265_RS24165 at 5886.597 kb on + strand, within CA265_RS24165 at 5886.652 kb on + strand, within CA265_RS24165 at 5886.652 kb on + strand, within CA265_RS24165 at 5886.653 kb on - strand, within CA265_RS24165 at 5886.735 kb on + strand, within CA265_RS24165 at 5886.776 kb on + strand at 5886.918 kb on - strand at 5886.971 kb on - strand at 5886.987 kb on - strand at 5886.987 kb on - strand at 5886.995 kb on - strand at 5886.995 kb on - strand at 5887.022 kb on - strand at 5887.022 kb on - strand at 5887.050 kb on - strand at 5887.170 kb on - strand, within CA265_RS24170 at 5887.264 kb on + strand, within CA265_RS24170 at 5887.335 kb on - strand, within CA265_RS24170 at 5887.375 kb on - strand, within CA265_RS24170 at 5887.475 kb on + strand, within CA265_RS24170 at 5887.475 kb on + strand at 5887.476 kb on - strand, within CA265_RS24170 at 5887.479 kb on + strand, within CA265_RS24170 at 5887.479 kb on + strand, within CA265_RS24170 at 5887.479 kb on + strand, within CA265_RS24170 at 5887.480 kb on - strand, within CA265_RS24170 at 5887.612 kb on + strand, within CA265_RS24170 at 5887.712 kb on + strand, within CA265_RS24170 at 5887.790 kb on + strand, within CA265_RS24170 at 5887.821 kb on - strand, within CA265_RS24170
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 5,885,209 + +0.1 5,885,224 - -0.1 5,885,239 + +1.4 5,885,278 - -0.3 5,885,334 + -0.3 5,885,366 + -0.2 5,885,367 - +0.2 5,885,376 + -1.9 5,885,376 + -4.3 5,885,377 - -0.0 5,885,377 - +0.3 5,885,377 - -0.4 5,885,473 + CA265_RS24160 0.14 -0.0 5,885,474 - CA265_RS24160 0.14 -0.8 5,885,551 - +0.8 5,885,570 - CA265_RS24160 0.26 -0.1 5,885,572 - CA265_RS24160 0.26 -1.0 5,885,572 - CA265_RS24160 0.26 -1.0 5,885,572 - CA265_RS24160 0.26 -0.7 5,885,572 - CA265_RS24160 0.26 +0.1 5,885,657 + CA265_RS24160 0.36 -1.0 5,885,771 - CA265_RS24160 0.50 -0.9 5,885,916 - CA265_RS24160 0.68 -0.1 5,885,969 - CA265_RS24160 0.74 +0.4 5,886,001 + CA265_RS24160 0.78 -1.6 5,886,002 - CA265_RS24160 0.78 -0.8 5,886,165 + +0.4 5,886,169 + -0.2 5,886,274 - CA265_RS24165 0.15 +1.4 5,886,311 - CA265_RS24165 0.21 +0.6 5,886,338 + CA265_RS24165 0.25 -1.0 5,886,349 - CA265_RS24165 0.27 -0.4 5,886,447 - CA265_RS24165 0.42 +0.4 5,886,459 - CA265_RS24165 0.43 +0.4 5,886,497 - CA265_RS24165 0.49 -0.4 5,886,505 + CA265_RS24165 0.50 -0.5 5,886,506 - CA265_RS24165 0.51 -0.3 5,886,507 + CA265_RS24165 0.51 -1.2 5,886,536 + CA265_RS24165 0.55 -0.5 5,886,579 + CA265_RS24165 0.62 -0.8 5,886,580 - CA265_RS24165 0.62 -0.3 5,886,597 + CA265_RS24165 0.65 -1.3 5,886,652 + CA265_RS24165 0.73 -0.1 5,886,652 + CA265_RS24165 0.73 +0.5 5,886,653 - CA265_RS24165 0.73 +0.3 5,886,735 + CA265_RS24165 0.86 -0.3 5,886,776 + -0.1 5,886,918 - +1.3 5,886,971 - +0.2 5,886,987 - +1.1 5,886,987 - +0.2 5,886,995 - -0.1 5,886,995 - -1.1 5,887,022 - +0.9 5,887,022 - +0.5 5,887,050 - +0.8 5,887,170 - CA265_RS24170 0.19 -1.2 5,887,264 + CA265_RS24170 0.27 -0.4 5,887,335 - CA265_RS24170 0.33 -0.1 5,887,375 - CA265_RS24170 0.36 +0.8 5,887,475 + CA265_RS24170 0.44 -0.4 5,887,475 + -0.0 5,887,476 - CA265_RS24170 0.45 -1.2 5,887,479 + CA265_RS24170 0.45 +1.0 5,887,479 + CA265_RS24170 0.45 +1.6 5,887,479 + CA265_RS24170 0.45 -0.2 5,887,480 - CA265_RS24170 0.45 +0.2 5,887,612 + CA265_RS24170 0.56 -0.2 5,887,712 + CA265_RS24170 0.64 +0.4 5,887,790 + CA265_RS24170 0.71 +0.3 5,887,821 - CA265_RS24170 0.73 +0.6
Or see this region's nucleotide sequence