Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS23515 and CA265_RS23520 are separated by 3 nucleotides CA265_RS23520 and CA265_RS23525 are separated by 79 nucleotides CA265_RS23525 and CA265_RS23530 are separated by 82 nucleotides CA265_RS23530 and CA265_RS23535 are separated by 226 nucleotides
CA265_RS23515: CA265_RS23515 - hypothetical protein, at 5,750,501 to 5,750,728
_RS23515
CA265_RS23520: CA265_RS23520 - phosphate ABC transporter substrate-binding protein, at 5,750,732 to 5,751,634
_RS23520
CA265_RS23525: CA265_RS23525 - hypothetical protein, at 5,751,714 to 5,753,429
_RS23525
CA265_RS23530: CA265_RS23530 - NADH-quinone oxidoreductase subunit A, at 5,753,512 to 5,753,883
_RS23530
CA265_RS23535: CA265_RS23535 - NADH dehydrogenase, at 5,754,110 to 5,754,652
_RS23535
Position (kb)
5751
5752
5753
5754 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5750.828 kb on - strand, within CA265_RS23520 at 5750.833 kb on + strand, within CA265_RS23520 at 5750.834 kb on - strand, within CA265_RS23520 at 5750.834 kb on - strand, within CA265_RS23520 at 5750.834 kb on - strand, within CA265_RS23520 at 5750.834 kb on - strand, within CA265_RS23520 at 5750.949 kb on + strand, within CA265_RS23520 at 5750.997 kb on + strand, within CA265_RS23520 at 5751.037 kb on + strand, within CA265_RS23520 at 5751.067 kb on - strand, within CA265_RS23520 at 5751.082 kb on - strand, within CA265_RS23520 at 5751.109 kb on + strand, within CA265_RS23520 at 5751.110 kb on - strand, within CA265_RS23520 at 5751.136 kb on + strand, within CA265_RS23520 at 5751.137 kb on - strand, within CA265_RS23520 at 5751.138 kb on + strand, within CA265_RS23520 at 5751.139 kb on - strand, within CA265_RS23520 at 5751.141 kb on + strand, within CA265_RS23520 at 5751.185 kb on + strand, within CA265_RS23520 at 5751.313 kb on + strand, within CA265_RS23520 at 5751.363 kb on - strand, within CA265_RS23520 at 5751.411 kb on + strand, within CA265_RS23520 at 5751.412 kb on - strand, within CA265_RS23520 at 5751.426 kb on - strand, within CA265_RS23520 at 5751.426 kb on - strand at 5751.451 kb on - strand, within CA265_RS23520 at 5751.483 kb on + strand, within CA265_RS23520 at 5751.484 kb on - strand, within CA265_RS23520 at 5751.484 kb on - strand, within CA265_RS23520 at 5751.541 kb on - strand, within CA265_RS23520 at 5751.742 kb on + strand at 5751.927 kb on - strand, within CA265_RS23525 at 5751.938 kb on + strand, within CA265_RS23525 at 5751.946 kb on + strand, within CA265_RS23525 at 5751.950 kb on + strand, within CA265_RS23525 at 5752.014 kb on - strand, within CA265_RS23525 at 5752.113 kb on + strand at 5752.144 kb on + strand, within CA265_RS23525 at 5752.145 kb on - strand, within CA265_RS23525 at 5752.149 kb on - strand, within CA265_RS23525 at 5752.248 kb on + strand, within CA265_RS23525 at 5752.249 kb on - strand, within CA265_RS23525 at 5752.279 kb on + strand, within CA265_RS23525 at 5752.279 kb on + strand, within CA265_RS23525 at 5752.280 kb on - strand, within CA265_RS23525 at 5752.288 kb on - strand, within CA265_RS23525 at 5752.399 kb on + strand, within CA265_RS23525 at 5752.400 kb on - strand, within CA265_RS23525 at 5752.400 kb on - strand, within CA265_RS23525 at 5752.419 kb on + strand, within CA265_RS23525 at 5752.450 kb on + strand, within CA265_RS23525 at 5752.451 kb on - strand, within CA265_RS23525 at 5752.456 kb on + strand, within CA265_RS23525 at 5752.457 kb on - strand, within CA265_RS23525 at 5752.457 kb on - strand, within CA265_RS23525 at 5752.482 kb on - strand, within CA265_RS23525 at 5752.532 kb on - strand, within CA265_RS23525 at 5752.638 kb on - strand, within CA265_RS23525 at 5752.687 kb on - strand, within CA265_RS23525 at 5752.817 kb on + strand, within CA265_RS23525 at 5752.822 kb on + strand, within CA265_RS23525 at 5752.822 kb on + strand, within CA265_RS23525 at 5752.879 kb on + strand, within CA265_RS23525 at 5752.911 kb on - strand, within CA265_RS23525 at 5752.923 kb on - strand, within CA265_RS23525 at 5752.983 kb on + strand, within CA265_RS23525 at 5752.983 kb on + strand, within CA265_RS23525 at 5752.984 kb on - strand, within CA265_RS23525 at 5752.989 kb on + strand, within CA265_RS23525 at 5752.990 kb on - strand, within CA265_RS23525 at 5752.992 kb on + strand, within CA265_RS23525 at 5752.995 kb on + strand, within CA265_RS23525 at 5753.130 kb on + strand, within CA265_RS23525 at 5753.131 kb on - strand, within CA265_RS23525 at 5753.131 kb on - strand, within CA265_RS23525 at 5753.148 kb on - strand, within CA265_RS23525 at 5753.211 kb on + strand, within CA265_RS23525 at 5753.286 kb on - strand at 5753.304 kb on + strand at 5753.305 kb on - strand at 5753.353 kb on + strand at 5753.428 kb on - strand at 5753.920 kb on + strand at 5754.055 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 5,750,828 - CA265_RS23520 0.11 +0.7 5,750,833 + CA265_RS23520 0.11 +0.3 5,750,834 - CA265_RS23520 0.11 +1.0 5,750,834 - CA265_RS23520 0.11 +0.6 5,750,834 - CA265_RS23520 0.11 +1.7 5,750,834 - CA265_RS23520 0.11 +0.4 5,750,949 + CA265_RS23520 0.24 +0.5 5,750,997 + CA265_RS23520 0.29 -0.3 5,751,037 + CA265_RS23520 0.34 +0.9 5,751,067 - CA265_RS23520 0.37 +1.0 5,751,082 - CA265_RS23520 0.39 +0.6 5,751,109 + CA265_RS23520 0.42 +0.7 5,751,110 - CA265_RS23520 0.42 +1.0 5,751,136 + CA265_RS23520 0.45 +1.1 5,751,137 - CA265_RS23520 0.45 +0.7 5,751,138 + CA265_RS23520 0.45 +0.2 5,751,139 - CA265_RS23520 0.45 -0.2 5,751,141 + CA265_RS23520 0.45 +0.7 5,751,185 + CA265_RS23520 0.50 +1.0 5,751,313 + CA265_RS23520 0.64 +0.4 5,751,363 - CA265_RS23520 0.70 +0.1 5,751,411 + CA265_RS23520 0.75 +0.3 5,751,412 - CA265_RS23520 0.75 +1.1 5,751,426 - CA265_RS23520 0.77 +0.8 5,751,426 - +2.7 5,751,451 - CA265_RS23520 0.80 +0.8 5,751,483 + CA265_RS23520 0.83 +0.1 5,751,484 - CA265_RS23520 0.83 +0.8 5,751,484 - CA265_RS23520 0.83 +0.4 5,751,541 - CA265_RS23520 0.90 +0.7 5,751,742 + -2.6 5,751,927 - CA265_RS23525 0.12 +0.7 5,751,938 + CA265_RS23525 0.13 -0.3 5,751,946 + CA265_RS23525 0.14 +0.6 5,751,950 + CA265_RS23525 0.14 +0.8 5,752,014 - CA265_RS23525 0.17 +0.8 5,752,113 + +2.7 5,752,144 + CA265_RS23525 0.25 +0.6 5,752,145 - CA265_RS23525 0.25 +0.2 5,752,149 - CA265_RS23525 0.25 +0.8 5,752,248 + CA265_RS23525 0.31 +0.1 5,752,249 - CA265_RS23525 0.31 +0.2 5,752,279 + CA265_RS23525 0.33 +0.1 5,752,279 + CA265_RS23525 0.33 +0.4 5,752,280 - CA265_RS23525 0.33 -0.9 5,752,288 - CA265_RS23525 0.33 +1.2 5,752,399 + CA265_RS23525 0.40 +0.6 5,752,400 - CA265_RS23525 0.40 +1.7 5,752,400 - CA265_RS23525 0.40 +1.0 5,752,419 + CA265_RS23525 0.41 +1.4 5,752,450 + CA265_RS23525 0.43 +0.8 5,752,451 - CA265_RS23525 0.43 -0.5 5,752,456 + CA265_RS23525 0.43 +0.0 5,752,457 - CA265_RS23525 0.43 +1.4 5,752,457 - CA265_RS23525 0.43 +0.9 5,752,482 - CA265_RS23525 0.45 +1.1 5,752,532 - CA265_RS23525 0.48 +2.2 5,752,638 - CA265_RS23525 0.54 +0.5 5,752,687 - CA265_RS23525 0.57 -0.8 5,752,817 + CA265_RS23525 0.64 -0.3 5,752,822 + CA265_RS23525 0.65 +0.8 5,752,822 + CA265_RS23525 0.65 +0.8 5,752,879 + CA265_RS23525 0.68 +0.1 5,752,911 - CA265_RS23525 0.70 +1.0 5,752,923 - CA265_RS23525 0.70 +0.3 5,752,983 + CA265_RS23525 0.74 +1.0 5,752,983 + CA265_RS23525 0.74 +1.7 5,752,984 - CA265_RS23525 0.74 -0.2 5,752,989 + CA265_RS23525 0.74 +1.7 5,752,990 - CA265_RS23525 0.74 -0.7 5,752,992 + CA265_RS23525 0.74 +0.7 5,752,995 + CA265_RS23525 0.75 +0.8 5,753,130 + CA265_RS23525 0.83 +1.1 5,753,131 - CA265_RS23525 0.83 +0.5 5,753,131 - CA265_RS23525 0.83 +2.2 5,753,148 - CA265_RS23525 0.84 +0.2 5,753,211 + CA265_RS23525 0.87 +0.8 5,753,286 - +0.2 5,753,304 + +0.8 5,753,305 - +0.4 5,753,353 + +1.5 5,753,428 - -0.0 5,753,920 + +0.8 5,754,055 + -3.0
Or see this region's nucleotide sequence