Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS13045 and CA265_RS13050 are separated by 4 nucleotides CA265_RS13050 and CA265_RS13055 are separated by 227 nucleotides CA265_RS13055 and CA265_RS13060 are separated by 147 nucleotides
CA265_RS13045: CA265_RS13045 - hypothetical protein, at 3,248,873 to 3,251,677
_RS13045
CA265_RS13050: CA265_RS13050 - hypothetical protein, at 3,251,682 to 3,251,960
_RS13050
CA265_RS13055: CA265_RS13055 - hypothetical protein, at 3,252,188 to 3,252,481
_RS13055
CA265_RS13060: CA265_RS13060 - hypothetical protein, at 3,252,629 to 3,253,351
_RS13060
Position (kb)
3251
3252 Strain fitness (log2 ratio)
-2
-1
0
1 at 3250.717 kb on + strand, within CA265_RS13045 at 3250.872 kb on + strand, within CA265_RS13045 at 3250.872 kb on + strand, within CA265_RS13045 at 3250.873 kb on - strand, within CA265_RS13045 at 3250.881 kb on + strand, within CA265_RS13045 at 3250.881 kb on + strand, within CA265_RS13045 at 3250.913 kb on + strand, within CA265_RS13045 at 3250.914 kb on - strand, within CA265_RS13045 at 3250.914 kb on - strand, within CA265_RS13045 at 3250.958 kb on + strand, within CA265_RS13045 at 3250.959 kb on - strand, within CA265_RS13045 at 3250.964 kb on - strand, within CA265_RS13045 at 3250.964 kb on - strand, within CA265_RS13045 at 3251.013 kb on - strand, within CA265_RS13045 at 3251.013 kb on - strand, within CA265_RS13045 at 3251.054 kb on + strand, within CA265_RS13045 at 3251.113 kb on + strand, within CA265_RS13045 at 3251.179 kb on - strand, within CA265_RS13045 at 3251.179 kb on - strand, within CA265_RS13045 at 3251.192 kb on + strand, within CA265_RS13045 at 3251.360 kb on + strand, within CA265_RS13045 at 3251.373 kb on + strand, within CA265_RS13045 at 3251.388 kb on + strand, within CA265_RS13045 at 3251.460 kb on - strand at 3251.540 kb on + strand at 3251.599 kb on - strand at 3251.650 kb on - strand at 3251.662 kb on + strand at 3251.668 kb on + strand at 3251.791 kb on + strand, within CA265_RS13050 at 3251.792 kb on - strand, within CA265_RS13050 at 3251.880 kb on + strand, within CA265_RS13050 at 3251.881 kb on - strand, within CA265_RS13050 at 3251.942 kb on + strand at 3251.949 kb on + strand at 3251.954 kb on + strand at 3251.954 kb on + strand at 3251.954 kb on + strand at 3251.966 kb on - strand at 3251.972 kb on - strand at 3251.972 kb on - strand at 3251.992 kb on + strand at 3252.054 kb on - strand at 3252.066 kb on + strand at 3252.143 kb on + strand at 3252.155 kb on + strand at 3252.155 kb on + strand at 3252.156 kb on - strand at 3252.214 kb on - strand at 3252.462 kb on + strand at 3252.462 kb on + strand at 3252.469 kb on + strand at 3252.472 kb on + strand at 3252.482 kb on - strand at 3252.539 kb on - strand at 3252.660 kb on - strand at 3252.783 kb on + strand, within CA265_RS13060 at 3252.813 kb on + strand, within CA265_RS13060 at 3252.876 kb on - strand, within CA265_RS13060 at 3252.941 kb on + strand, within CA265_RS13060
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 3,250,717 + CA265_RS13045 0.66 -0.4 3,250,872 + CA265_RS13045 0.71 +0.4 3,250,872 + CA265_RS13045 0.71 -0.0 3,250,873 - CA265_RS13045 0.71 +0.0 3,250,881 + CA265_RS13045 0.72 -1.4 3,250,881 + CA265_RS13045 0.72 +0.4 3,250,913 + CA265_RS13045 0.73 +0.0 3,250,914 - CA265_RS13045 0.73 -0.3 3,250,914 - CA265_RS13045 0.73 -0.7 3,250,958 + CA265_RS13045 0.74 +0.5 3,250,959 - CA265_RS13045 0.74 -0.9 3,250,964 - CA265_RS13045 0.75 +0.6 3,250,964 - CA265_RS13045 0.75 +0.5 3,251,013 - CA265_RS13045 0.76 +0.5 3,251,013 - CA265_RS13045 0.76 +0.1 3,251,054 + CA265_RS13045 0.78 -0.1 3,251,113 + CA265_RS13045 0.80 -0.1 3,251,179 - CA265_RS13045 0.82 +0.1 3,251,179 - CA265_RS13045 0.82 -0.1 3,251,192 + CA265_RS13045 0.83 -0.9 3,251,360 + CA265_RS13045 0.89 -0.2 3,251,373 + CA265_RS13045 0.89 +0.2 3,251,388 + CA265_RS13045 0.90 -0.2 3,251,460 - -0.3 3,251,540 + -0.1 3,251,599 - -0.5 3,251,650 - +0.3 3,251,662 + +0.2 3,251,668 + +0.2 3,251,791 + CA265_RS13050 0.39 +0.1 3,251,792 - CA265_RS13050 0.39 -0.3 3,251,880 + CA265_RS13050 0.71 +0.4 3,251,881 - CA265_RS13050 0.71 +0.3 3,251,942 + -0.7 3,251,949 + -0.7 3,251,954 + +1.0 3,251,954 + -1.2 3,251,954 + -0.4 3,251,966 - -0.0 3,251,972 - +0.7 3,251,972 - +0.2 3,251,992 + -0.4 3,252,054 - +0.0 3,252,066 + +0.5 3,252,143 + +1.3 3,252,155 + -0.7 3,252,155 + +0.3 3,252,156 - -0.5 3,252,214 - +0.1 3,252,462 + -1.8 3,252,462 + -0.3 3,252,469 + +0.4 3,252,472 + +0.4 3,252,482 - -0.1 3,252,539 - +0.2 3,252,660 - +0.9 3,252,783 + CA265_RS13060 0.21 +0.4 3,252,813 + CA265_RS13060 0.25 +0.3 3,252,876 - CA265_RS13060 0.34 +0.0 3,252,941 + CA265_RS13060 0.43 +1.1
Or see this region's nucleotide sequence