Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS11760 and CA265_RS11765 are separated by 3 nucleotides CA265_RS11765 and CA265_RS11770 are separated by 12 nucleotides
CA265_RS11760: CA265_RS11760 - mobilization protein, at 2,913,852 to 2,915,810
_RS11760
CA265_RS11765: CA265_RS11765 - conjugative transposon protein TraN, at 2,915,814 to 2,916,659
_RS11765
CA265_RS11770: CA265_RS11770 - hypothetical protein, at 2,916,672 to 2,917,862
_RS11770
Position (kb)
2915
2916
2917 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2914.815 kb on - strand, within CA265_RS11760 at 2914.815 kb on - strand, within CA265_RS11760 at 2914.961 kb on + strand, within CA265_RS11760 at 2914.961 kb on + strand, within CA265_RS11760 at 2914.961 kb on + strand, within CA265_RS11760 at 2914.963 kb on + strand, within CA265_RS11760 at 2915.261 kb on + strand, within CA265_RS11760 at 2915.403 kb on - strand, within CA265_RS11760 at 2915.413 kb on - strand, within CA265_RS11760 at 2915.415 kb on - strand, within CA265_RS11760 at 2915.434 kb on - strand, within CA265_RS11760 at 2915.465 kb on - strand, within CA265_RS11760 at 2915.516 kb on - strand, within CA265_RS11760 at 2915.516 kb on - strand, within CA265_RS11760 at 2915.535 kb on + strand, within CA265_RS11760 at 2915.641 kb on - strand at 2915.741 kb on + strand at 2915.745 kb on + strand at 2915.745 kb on + strand at 2915.751 kb on - strand at 2915.755 kb on + strand at 2915.798 kb on + strand at 2915.812 kb on - strand at 2915.816 kb on - strand at 2915.823 kb on - strand at 2915.829 kb on + strand at 2915.844 kb on + strand at 2915.844 kb on + strand at 2915.845 kb on - strand at 2915.924 kb on + strand, within CA265_RS11765 at 2915.924 kb on + strand, within CA265_RS11765 at 2915.924 kb on + strand, within CA265_RS11765 at 2915.958 kb on + strand, within CA265_RS11765 at 2915.968 kb on + strand, within CA265_RS11765 at 2916.044 kb on + strand, within CA265_RS11765 at 2916.091 kb on + strand, within CA265_RS11765 at 2916.162 kb on - strand, within CA265_RS11765 at 2916.196 kb on - strand, within CA265_RS11765 at 2916.239 kb on + strand, within CA265_RS11765 at 2916.239 kb on + strand, within CA265_RS11765 at 2916.240 kb on - strand, within CA265_RS11765 at 2916.362 kb on - strand, within CA265_RS11765 at 2916.392 kb on + strand, within CA265_RS11765 at 2916.418 kb on - strand, within CA265_RS11765 at 2916.491 kb on + strand, within CA265_RS11765 at 2916.492 kb on - strand, within CA265_RS11765 at 2916.500 kb on - strand, within CA265_RS11765 at 2916.500 kb on - strand, within CA265_RS11765 at 2916.522 kb on - strand, within CA265_RS11765 at 2916.584 kb on + strand at 2916.646 kb on - strand at 2916.719 kb on - strand at 2916.719 kb on - strand at 2916.770 kb on + strand at 2916.771 kb on - strand at 2916.771 kb on - strand at 2916.786 kb on - strand at 2916.820 kb on - strand, within CA265_RS11770 at 2916.905 kb on - strand, within CA265_RS11770 at 2916.905 kb on - strand, within CA265_RS11770 at 2917.067 kb on - strand, within CA265_RS11770 at 2917.068 kb on + strand, within CA265_RS11770 at 2917.130 kb on + strand, within CA265_RS11770 at 2917.274 kb on + strand, within CA265_RS11770 at 2917.276 kb on + strand, within CA265_RS11770 at 2917.277 kb on - strand, within CA265_RS11770 at 2917.311 kb on - strand, within CA265_RS11770 at 2917.313 kb on - strand, within CA265_RS11770 at 2917.466 kb on + strand, within CA265_RS11770 at 2917.467 kb on - strand, within CA265_RS11770 at 2917.469 kb on + strand, within CA265_RS11770 at 2917.470 kb on - strand, within CA265_RS11770 at 2917.477 kb on + strand, within CA265_RS11770 at 2917.516 kb on + strand, within CA265_RS11770 at 2917.544 kb on + strand, within CA265_RS11770 at 2917.576 kb on + strand, within CA265_RS11770 at 2917.576 kb on + strand, within CA265_RS11770 at 2917.580 kb on + strand, within CA265_RS11770 at 2917.658 kb on + strand, within CA265_RS11770
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 2,914,815 - CA265_RS11760 0.49 -3.0 2,914,815 - CA265_RS11760 0.49 -2.5 2,914,961 + CA265_RS11760 0.57 +0.1 2,914,961 + CA265_RS11760 0.57 +0.3 2,914,961 + CA265_RS11760 0.57 +0.6 2,914,963 + CA265_RS11760 0.57 +0.5 2,915,261 + CA265_RS11760 0.72 +0.0 2,915,403 - CA265_RS11760 0.79 -3.1 2,915,413 - CA265_RS11760 0.80 -1.2 2,915,415 - CA265_RS11760 0.80 -1.2 2,915,434 - CA265_RS11760 0.81 -2.1 2,915,465 - CA265_RS11760 0.82 -3.7 2,915,516 - CA265_RS11760 0.85 -1.7 2,915,516 - CA265_RS11760 0.85 +0.1 2,915,535 + CA265_RS11760 0.86 +0.8 2,915,641 - -0.3 2,915,741 + +0.0 2,915,745 + -0.8 2,915,745 + -0.5 2,915,751 - -2.5 2,915,755 + +0.1 2,915,798 + +0.5 2,915,812 - -1.9 2,915,816 - -0.5 2,915,823 - -0.3 2,915,829 + -0.4 2,915,844 + -0.1 2,915,844 + -1.4 2,915,845 - -1.4 2,915,924 + CA265_RS11765 0.13 +0.1 2,915,924 + CA265_RS11765 0.13 +0.7 2,915,924 + CA265_RS11765 0.13 +0.3 2,915,958 + CA265_RS11765 0.17 -0.6 2,915,968 + CA265_RS11765 0.18 +0.3 2,916,044 + CA265_RS11765 0.27 +0.4 2,916,091 + CA265_RS11765 0.33 -0.8 2,916,162 - CA265_RS11765 0.41 +1.0 2,916,196 - CA265_RS11765 0.45 -0.5 2,916,239 + CA265_RS11765 0.50 +0.8 2,916,239 + CA265_RS11765 0.50 +0.4 2,916,240 - CA265_RS11765 0.50 -1.2 2,916,362 - CA265_RS11765 0.65 -1.6 2,916,392 + CA265_RS11765 0.68 +0.1 2,916,418 - CA265_RS11765 0.71 -1.0 2,916,491 + CA265_RS11765 0.80 -0.7 2,916,492 - CA265_RS11765 0.80 -1.0 2,916,500 - CA265_RS11765 0.81 -0.2 2,916,500 - CA265_RS11765 0.81 -0.8 2,916,522 - CA265_RS11765 0.84 -1.0 2,916,584 + -0.7 2,916,646 - -1.7 2,916,719 - +1.5 2,916,719 - -0.7 2,916,770 + +0.4 2,916,771 - -2.6 2,916,771 - -1.2 2,916,786 - -2.5 2,916,820 - CA265_RS11770 0.12 -2.7 2,916,905 - CA265_RS11770 0.20 -2.6 2,916,905 - CA265_RS11770 0.20 -2.9 2,917,067 - CA265_RS11770 0.33 -1.5 2,917,068 + CA265_RS11770 0.33 +0.2 2,917,130 + CA265_RS11770 0.38 +0.3 2,917,274 + CA265_RS11770 0.51 +0.4 2,917,276 + CA265_RS11770 0.51 +0.6 2,917,277 - CA265_RS11770 0.51 -1.8 2,917,311 - CA265_RS11770 0.54 -1.1 2,917,313 - CA265_RS11770 0.54 +0.8 2,917,466 + CA265_RS11770 0.67 -0.1 2,917,467 - CA265_RS11770 0.67 -1.8 2,917,469 + CA265_RS11770 0.67 +0.0 2,917,470 - CA265_RS11770 0.67 -1.9 2,917,477 + CA265_RS11770 0.68 +0.6 2,917,516 + CA265_RS11770 0.71 +0.1 2,917,544 + CA265_RS11770 0.73 -0.2 2,917,576 + CA265_RS11770 0.76 +1.2 2,917,576 + CA265_RS11770 0.76 -0.5 2,917,580 + CA265_RS11770 0.76 -0.3 2,917,658 + CA265_RS11770 0.83 +1.8
Or see this region's nucleotide sequence