Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS08995 and CA265_RS09000 are separated by 249 nucleotides CA265_RS09000 and CA265_RS09005 are separated by 11 nucleotides CA265_RS09005 and CA265_RS09010 are separated by 79 nucleotides
CA265_RS08995: CA265_RS08995 - TonB-dependent receptor, at 2,243,367 to 2,245,793
_RS08995
CA265_RS09000: CA265_RS09000 - hypothetical protein, at 2,246,043 to 2,246,228
_RS09000
CA265_RS09005: CA265_RS09005 - phosphoserine transaminase, at 2,246,240 to 2,247,313
_RS09005
CA265_RS09010: CA265_RS09010 - 3-phosphoglycerate dehydrogenase, at 2,247,393 to 2,248,340
_RS09010
Position (kb)
2246
2247
2248 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2245.290 kb on + strand, within CA265_RS08995 at 2245.338 kb on - strand, within CA265_RS08995 at 2245.450 kb on - strand, within CA265_RS08995 at 2245.451 kb on + strand, within CA265_RS08995 at 2245.462 kb on + strand, within CA265_RS08995 at 2245.493 kb on - strand, within CA265_RS08995 at 2245.545 kb on - strand, within CA265_RS08995 at 2245.552 kb on + strand at 2245.552 kb on + strand at 2245.556 kb on + strand at 2245.634 kb on + strand at 2245.636 kb on + strand at 2245.659 kb on - strand at 2245.687 kb on - strand at 2245.687 kb on - strand at 2245.706 kb on - strand at 2245.710 kb on - strand at 2245.710 kb on - strand at 2245.719 kb on + strand at 2245.719 kb on + strand at 2245.720 kb on - strand at 2245.731 kb on - strand at 2245.734 kb on + strand at 2245.893 kb on - strand at 2245.897 kb on - strand at 2245.950 kb on + strand at 2246.045 kb on - strand at 2246.169 kb on - strand, within CA265_RS09000 at 2246.189 kb on + strand, within CA265_RS09000 at 2246.231 kb on + strand at 2247.347 kb on - strand at 2247.386 kb on + strand at 2247.587 kb on - strand, within CA265_RS09010 at 2247.617 kb on + strand, within CA265_RS09010 at 2247.630 kb on + strand, within CA265_RS09010 at 2247.713 kb on - strand, within CA265_RS09010 at 2247.799 kb on - strand, within CA265_RS09010 at 2247.836 kb on + strand, within CA265_RS09010 at 2247.867 kb on - strand, within CA265_RS09010 at 2247.916 kb on + strand, within CA265_RS09010 at 2247.916 kb on + strand, within CA265_RS09010 at 2247.916 kb on + strand, within CA265_RS09010 at 2247.917 kb on - strand, within CA265_RS09010 at 2248.008 kb on - strand, within CA265_RS09010 at 2248.008 kb on - strand, within CA265_RS09010 at 2248.030 kb on + strand, within CA265_RS09010 at 2248.100 kb on + strand, within CA265_RS09010 at 2248.101 kb on - strand, within CA265_RS09010 at 2248.156 kb on + strand, within CA265_RS09010 at 2248.259 kb on + strand at 2248.260 kb on - strand at 2248.260 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 2,245,290 + CA265_RS08995 0.79 +0.4 2,245,338 - CA265_RS08995 0.81 +0.5 2,245,450 - CA265_RS08995 0.86 +1.2 2,245,451 + CA265_RS08995 0.86 -0.2 2,245,462 + CA265_RS08995 0.86 -0.7 2,245,493 - CA265_RS08995 0.88 -0.8 2,245,545 - CA265_RS08995 0.90 +0.7 2,245,552 + +0.9 2,245,552 + +0.4 2,245,556 + +0.1 2,245,634 + -0.2 2,245,636 + +0.3 2,245,659 - +0.6 2,245,687 - +0.6 2,245,687 - +0.4 2,245,706 - +0.3 2,245,710 - +0.6 2,245,710 - -0.1 2,245,719 + +0.9 2,245,719 + +0.2 2,245,720 - +1.1 2,245,731 - +0.4 2,245,734 + +2.8 2,245,893 - +0.7 2,245,897 - +1.3 2,245,950 + -0.2 2,246,045 - +0.2 2,246,169 - CA265_RS09000 0.68 +1.1 2,246,189 + CA265_RS09000 0.78 +0.3 2,246,231 + +1.2 2,247,347 - +0.9 2,247,386 + -0.1 2,247,587 - CA265_RS09010 0.20 +0.8 2,247,617 + CA265_RS09010 0.24 +0.3 2,247,630 + CA265_RS09010 0.25 -0.6 2,247,713 - CA265_RS09010 0.34 -0.2 2,247,799 - CA265_RS09010 0.43 +0.1 2,247,836 + CA265_RS09010 0.47 -0.3 2,247,867 - CA265_RS09010 0.50 +2.2 2,247,916 + CA265_RS09010 0.55 +1.0 2,247,916 + CA265_RS09010 0.55 +0.4 2,247,916 + CA265_RS09010 0.55 +0.4 2,247,917 - CA265_RS09010 0.55 -0.3 2,248,008 - CA265_RS09010 0.65 +1.7 2,248,008 - CA265_RS09010 0.65 +0.6 2,248,030 + CA265_RS09010 0.67 +0.2 2,248,100 + CA265_RS09010 0.75 -1.0 2,248,101 - CA265_RS09010 0.75 +2.1 2,248,156 + CA265_RS09010 0.80 +3.3 2,248,259 + +1.4 2,248,260 - -0.2 2,248,260 - -0.6
Or see this region's nucleotide sequence