Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06005 and CA265_RS06010 overlap by 11 nucleotides CA265_RS06010 and CA265_RS06015 are separated by 1 nucleotides CA265_RS06015 and CA265_RS06020 are separated by 3 nucleotides
CA265_RS06005: CA265_RS06005 - transposase, at 1,476,810 to 1,477,355
_RS06005
CA265_RS06010: CA265_RS06010 - hypothetical protein, at 1,477,345 to 1,477,647
_RS06010
CA265_RS06015: CA265_RS06015 - hypothetical protein, at 1,477,649 to 1,478,296
_RS06015
CA265_RS06020: CA265_RS06020 - DNA polymerase III, at 1,478,300 to 1,478,848
_RS06020
Position (kb)
1477
1478 Strain fitness (log2 ratio)
-2
-1
0
1 at 1476.380 kb on + strand at 1476.381 kb on - strand at 1476.381 kb on - strand at 1476.381 kb on - strand at 1476.381 kb on - strand at 1476.382 kb on + strand at 1476.428 kb on + strand at 1476.429 kb on - strand at 1476.441 kb on - strand at 1476.443 kb on + strand at 1476.443 kb on + strand at 1476.443 kb on + strand at 1476.443 kb on + strand at 1476.444 kb on - strand at 1476.497 kb on - strand at 1476.559 kb on - strand at 1476.585 kb on - strand at 1476.672 kb on - strand at 1476.684 kb on - strand at 1476.690 kb on - strand at 1476.833 kb on + strand at 1476.871 kb on + strand, within CA265_RS06005 at 1476.880 kb on + strand, within CA265_RS06005 at 1476.894 kb on - strand, within CA265_RS06005 at 1476.964 kb on - strand, within CA265_RS06005 at 1476.975 kb on - strand, within CA265_RS06005 at 1476.978 kb on - strand, within CA265_RS06005 at 1476.991 kb on + strand, within CA265_RS06005 at 1476.991 kb on + strand, within CA265_RS06005 at 1476.992 kb on - strand, within CA265_RS06005 at 1477.034 kb on - strand, within CA265_RS06005 at 1477.034 kb on - strand, within CA265_RS06005 at 1477.034 kb on - strand, within CA265_RS06005 at 1477.034 kb on - strand, within CA265_RS06005 at 1477.053 kb on + strand, within CA265_RS06005 at 1477.056 kb on - strand, within CA265_RS06005 at 1477.092 kb on - strand, within CA265_RS06005 at 1477.191 kb on - strand, within CA265_RS06005 at 1477.199 kb on - strand, within CA265_RS06005 at 1477.221 kb on - strand, within CA265_RS06005 at 1477.251 kb on - strand, within CA265_RS06005 at 1477.296 kb on + strand, within CA265_RS06005 at 1477.309 kb on + strand at 1477.309 kb on + strand at 1477.314 kb on + strand at 1477.337 kb on - strand at 1477.365 kb on - strand at 1477.435 kb on - strand, within CA265_RS06010 at 1477.466 kb on - strand, within CA265_RS06010 at 1477.512 kb on - strand, within CA265_RS06010 at 1477.578 kb on + strand, within CA265_RS06010 at 1477.625 kb on - strand at 1477.636 kb on - strand at 1477.663 kb on + strand at 1477.669 kb on + strand at 1477.670 kb on - strand at 1477.670 kb on - strand at 1477.730 kb on - strand, within CA265_RS06015 at 1477.772 kb on + strand, within CA265_RS06015 at 1477.773 kb on - strand, within CA265_RS06015 at 1477.773 kb on - strand, within CA265_RS06015 at 1477.776 kb on - strand, within CA265_RS06015 at 1477.792 kb on + strand, within CA265_RS06015 at 1477.835 kb on - strand, within CA265_RS06015 at 1477.885 kb on + strand, within CA265_RS06015 at 1477.906 kb on + strand, within CA265_RS06015 at 1477.907 kb on - strand, within CA265_RS06015 at 1477.946 kb on - strand, within CA265_RS06015 at 1477.957 kb on + strand, within CA265_RS06015 at 1478.004 kb on + strand, within CA265_RS06015 at 1478.009 kb on - strand, within CA265_RS06015 at 1478.014 kb on + strand, within CA265_RS06015 at 1478.014 kb on + strand, within CA265_RS06015 at 1478.015 kb on - strand, within CA265_RS06015 at 1478.015 kb on - strand, within CA265_RS06015 at 1478.070 kb on - strand, within CA265_RS06015 at 1478.078 kb on - strand, within CA265_RS06015 at 1478.080 kb on - strand, within CA265_RS06015 at 1478.130 kb on + strand, within CA265_RS06015 at 1478.249 kb on + strand at 1478.250 kb on - strand at 1478.252 kb on + strand at 1478.252 kb on + strand at 1478.281 kb on + strand at 1478.338 kb on - strand at 1478.374 kb on + strand, within CA265_RS06020 at 1478.393 kb on + strand, within CA265_RS06020 at 1478.418 kb on + strand, within CA265_RS06020 at 1478.418 kb on + strand, within CA265_RS06020 at 1478.418 kb on + strand, within CA265_RS06020 at 1478.418 kb on + strand, within CA265_RS06020 at 1478.419 kb on - strand, within CA265_RS06020 at 1478.419 kb on - strand, within CA265_RS06020 at 1478.528 kb on - strand, within CA265_RS06020 at 1478.537 kb on - strand, within CA265_RS06020 at 1478.566 kb on - strand, within CA265_RS06020 at 1478.576 kb on + strand, within CA265_RS06020 at 1478.581 kb on - strand, within CA265_RS06020 at 1478.622 kb on + strand, within CA265_RS06020
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 1,476,380 + -1.2 1,476,381 - +0.5 1,476,381 - +0.0 1,476,381 - -0.8 1,476,381 - +0.1 1,476,382 + -0.2 1,476,428 + -0.6 1,476,429 - -0.6 1,476,441 - -0.3 1,476,443 + +0.5 1,476,443 + -0.7 1,476,443 + -0.6 1,476,443 + +1.2 1,476,444 - -0.4 1,476,497 - -0.3 1,476,559 - -1.1 1,476,585 - -0.1 1,476,672 - -1.4 1,476,684 - -0.8 1,476,690 - -0.6 1,476,833 + -0.2 1,476,871 + CA265_RS06005 0.11 -0.1 1,476,880 + CA265_RS06005 0.13 -0.3 1,476,894 - CA265_RS06005 0.15 +0.1 1,476,964 - CA265_RS06005 0.28 +0.6 1,476,975 - CA265_RS06005 0.30 -0.1 1,476,978 - CA265_RS06005 0.31 -0.8 1,476,991 + CA265_RS06005 0.33 -0.7 1,476,991 + CA265_RS06005 0.33 -1.2 1,476,992 - CA265_RS06005 0.33 -0.9 1,477,034 - CA265_RS06005 0.41 -0.6 1,477,034 - CA265_RS06005 0.41 +0.3 1,477,034 - CA265_RS06005 0.41 +0.1 1,477,034 - CA265_RS06005 0.41 +0.3 1,477,053 + CA265_RS06005 0.45 -0.1 1,477,056 - CA265_RS06005 0.45 +0.1 1,477,092 - CA265_RS06005 0.52 +0.6 1,477,191 - CA265_RS06005 0.70 +0.2 1,477,199 - CA265_RS06005 0.71 -0.7 1,477,221 - CA265_RS06005 0.75 +0.7 1,477,251 - CA265_RS06005 0.81 +0.1 1,477,296 + CA265_RS06005 0.89 -0.3 1,477,309 + -0.5 1,477,309 + -1.3 1,477,314 + +0.7 1,477,337 - +0.1 1,477,365 - -0.6 1,477,435 - CA265_RS06010 0.30 +0.1 1,477,466 - CA265_RS06010 0.40 +0.3 1,477,512 - CA265_RS06010 0.55 +0.4 1,477,578 + CA265_RS06010 0.77 +1.1 1,477,625 - -1.3 1,477,636 - -0.2 1,477,663 + +0.1 1,477,669 + -0.1 1,477,670 - +0.6 1,477,670 - -0.7 1,477,730 - CA265_RS06015 0.12 +0.3 1,477,772 + CA265_RS06015 0.19 +0.6 1,477,773 - CA265_RS06015 0.19 -0.9 1,477,773 - CA265_RS06015 0.19 +0.2 1,477,776 - CA265_RS06015 0.20 +1.0 1,477,792 + CA265_RS06015 0.22 -0.2 1,477,835 - CA265_RS06015 0.29 +0.1 1,477,885 + CA265_RS06015 0.36 -0.5 1,477,906 + CA265_RS06015 0.40 +0.3 1,477,907 - CA265_RS06015 0.40 -0.4 1,477,946 - CA265_RS06015 0.46 +0.1 1,477,957 + CA265_RS06015 0.48 -0.5 1,478,004 + CA265_RS06015 0.55 -0.1 1,478,009 - CA265_RS06015 0.56 -0.0 1,478,014 + CA265_RS06015 0.56 -0.5 1,478,014 + CA265_RS06015 0.56 -0.9 1,478,015 - CA265_RS06015 0.56 +0.3 1,478,015 - CA265_RS06015 0.56 +0.5 1,478,070 - CA265_RS06015 0.65 -0.6 1,478,078 - CA265_RS06015 0.66 -0.3 1,478,080 - CA265_RS06015 0.67 -0.2 1,478,130 + CA265_RS06015 0.74 +0.7 1,478,249 + -0.1 1,478,250 - +0.1 1,478,252 + +0.2 1,478,252 + -0.2 1,478,281 + +0.1 1,478,338 - -0.2 1,478,374 + CA265_RS06020 0.13 -0.1 1,478,393 + CA265_RS06020 0.17 +0.4 1,478,418 + CA265_RS06020 0.21 -0.9 1,478,418 + CA265_RS06020 0.21 -0.3 1,478,418 + CA265_RS06020 0.21 +0.8 1,478,418 + CA265_RS06020 0.21 -1.0 1,478,419 - CA265_RS06020 0.22 +0.8 1,478,419 - CA265_RS06020 0.22 -0.1 1,478,528 - CA265_RS06020 0.42 +0.9 1,478,537 - CA265_RS06020 0.43 -0.1 1,478,566 - CA265_RS06020 0.48 -2.0 1,478,576 + CA265_RS06020 0.50 +0.5 1,478,581 - CA265_RS06020 0.51 -0.5 1,478,622 + CA265_RS06020 0.59 -0.7
Or see this region's nucleotide sequence