Experiment: R2A with A22 0.04 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS04025 and CA265_RS04030 are separated by 87 nucleotides CA265_RS04030 and CA265_RS04035 are separated by 30 nucleotides
CA265_RS04025: CA265_RS04025 - glycoside hydrolase family 2, at 963,509 to 966,673
_RS04025
CA265_RS04030: CA265_RS04030 - alpha-galactosidase, at 966,761 to 967,960
_RS04030
CA265_RS04035: CA265_RS04035 - hypothetical protein, at 967,991 to 969,499
_RS04035
Position (kb)
966
967
968 Strain fitness (log2 ratio)
-2
-1
0
1 at 965.777 kb on + strand, within CA265_RS04025 at 965.849 kb on - strand, within CA265_RS04025 at 965.888 kb on + strand, within CA265_RS04025 at 965.889 kb on - strand, within CA265_RS04025 at 965.889 kb on - strand, within CA265_RS04025 at 965.988 kb on + strand, within CA265_RS04025 at 965.989 kb on - strand, within CA265_RS04025 at 966.007 kb on - strand, within CA265_RS04025 at 966.016 kb on + strand, within CA265_RS04025 at 966.022 kb on + strand, within CA265_RS04025 at 966.022 kb on + strand, within CA265_RS04025 at 966.023 kb on - strand, within CA265_RS04025 at 966.028 kb on + strand, within CA265_RS04025 at 966.035 kb on - strand, within CA265_RS04025 at 966.044 kb on - strand, within CA265_RS04025 at 966.073 kb on - strand, within CA265_RS04025 at 966.090 kb on + strand, within CA265_RS04025 at 966.091 kb on - strand, within CA265_RS04025 at 966.153 kb on + strand, within CA265_RS04025 at 966.166 kb on + strand, within CA265_RS04025 at 966.167 kb on - strand, within CA265_RS04025 at 966.250 kb on + strand, within CA265_RS04025 at 966.252 kb on - strand, within CA265_RS04025 at 966.300 kb on + strand, within CA265_RS04025 at 966.308 kb on - strand, within CA265_RS04025 at 966.308 kb on - strand, within CA265_RS04025 at 966.314 kb on - strand, within CA265_RS04025 at 966.370 kb on + strand at 966.404 kb on - strand at 966.483 kb on + strand at 966.529 kb on - strand at 966.570 kb on + strand at 966.571 kb on - strand at 966.602 kb on - strand at 966.650 kb on + strand at 966.651 kb on - strand at 966.654 kb on - strand at 966.656 kb on - strand at 966.671 kb on + strand at 966.672 kb on - strand at 966.704 kb on + strand at 966.705 kb on - strand at 966.737 kb on + strand at 966.737 kb on + strand at 966.737 kb on + strand at 966.756 kb on + strand at 966.756 kb on + strand at 966.757 kb on - strand at 966.789 kb on + strand at 966.895 kb on + strand, within CA265_RS04030 at 967.039 kb on - strand, within CA265_RS04030 at 967.088 kb on + strand, within CA265_RS04030 at 967.089 kb on - strand, within CA265_RS04030 at 967.123 kb on + strand, within CA265_RS04030 at 967.184 kb on + strand, within CA265_RS04030 at 967.282 kb on + strand, within CA265_RS04030 at 967.282 kb on + strand, within CA265_RS04030 at 967.282 kb on + strand, within CA265_RS04030 at 967.282 kb on + strand, within CA265_RS04030 at 967.294 kb on + strand, within CA265_RS04030 at 967.295 kb on - strand, within CA265_RS04030 at 967.352 kb on - strand, within CA265_RS04030 at 967.367 kb on + strand, within CA265_RS04030 at 967.368 kb on - strand, within CA265_RS04030 at 967.381 kb on + strand, within CA265_RS04030 at 967.381 kb on - strand, within CA265_RS04030 at 967.417 kb on + strand, within CA265_RS04030 at 967.417 kb on + strand, within CA265_RS04030 at 967.418 kb on - strand, within CA265_RS04030 at 967.418 kb on - strand, within CA265_RS04030 at 967.418 kb on - strand, within CA265_RS04030 at 967.421 kb on + strand, within CA265_RS04030 at 967.506 kb on + strand, within CA265_RS04030 at 967.506 kb on + strand, within CA265_RS04030 at 967.506 kb on + strand, within CA265_RS04030 at 967.538 kb on - strand, within CA265_RS04030 at 967.587 kb on - strand, within CA265_RS04030 at 967.597 kb on + strand, within CA265_RS04030 at 967.598 kb on - strand, within CA265_RS04030 at 967.609 kb on - strand, within CA265_RS04030 at 967.675 kb on - strand, within CA265_RS04030 at 967.853 kb on + strand at 967.853 kb on + strand at 967.885 kb on - strand at 967.917 kb on + strand at 967.959 kb on - strand at 967.970 kb on + strand at 968.002 kb on - strand at 968.080 kb on + strand at 968.170 kb on + strand, within CA265_RS04035 at 968.171 kb on - strand, within CA265_RS04035 at 968.291 kb on - strand, within CA265_RS04035 at 968.336 kb on + strand, within CA265_RS04035 at 968.336 kb on + strand, within CA265_RS04035 at 968.337 kb on - strand, within CA265_RS04035 at 968.337 kb on - strand, within CA265_RS04035 at 968.370 kb on - strand, within CA265_RS04035 at 968.403 kb on + strand, within CA265_RS04035 at 968.403 kb on + strand, within CA265_RS04035 at 968.404 kb on - strand, within CA265_RS04035 at 968.412 kb on + strand, within CA265_RS04035 at 968.434 kb on + strand, within CA265_RS04035 at 968.434 kb on + strand, within CA265_RS04035 at 968.435 kb on - strand, within CA265_RS04035 at 968.451 kb on - strand, within CA265_RS04035 at 968.457 kb on + strand, within CA265_RS04035 at 968.515 kb on - strand, within CA265_RS04035 at 968.559 kb on - strand, within CA265_RS04035 at 968.561 kb on - strand, within CA265_RS04035 at 968.561 kb on - strand, within CA265_RS04035 at 968.608 kb on + strand, within CA265_RS04035 at 968.677 kb on - strand, within CA265_RS04035 at 968.784 kb on + strand, within CA265_RS04035 at 968.872 kb on + strand, within CA265_RS04035 at 968.872 kb on + strand, within CA265_RS04035 at 968.885 kb on + strand, within CA265_RS04035 at 968.886 kb on - strand, within CA265_RS04035 at 968.886 kb on - strand, within CA265_RS04035
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with A22 0.04 mg/ml remove 965,777 + CA265_RS04025 0.72 -1.7 965,849 - CA265_RS04025 0.74 +1.0 965,888 + CA265_RS04025 0.75 -0.5 965,889 - CA265_RS04025 0.75 +0.3 965,889 - CA265_RS04025 0.75 +0.5 965,988 + CA265_RS04025 0.78 -0.4 965,989 - CA265_RS04025 0.78 -0.8 966,007 - CA265_RS04025 0.79 -0.5 966,016 + CA265_RS04025 0.79 +0.2 966,022 + CA265_RS04025 0.79 -0.9 966,022 + CA265_RS04025 0.79 -0.1 966,023 - CA265_RS04025 0.79 +0.8 966,028 + CA265_RS04025 0.80 +0.1 966,035 - CA265_RS04025 0.80 -0.3 966,044 - CA265_RS04025 0.80 +0.3 966,073 - CA265_RS04025 0.81 -0.1 966,090 + CA265_RS04025 0.82 -0.2 966,091 - CA265_RS04025 0.82 +1.4 966,153 + CA265_RS04025 0.84 +0.4 966,166 + CA265_RS04025 0.84 -0.2 966,167 - CA265_RS04025 0.84 +0.1 966,250 + CA265_RS04025 0.87 -0.1 966,252 - CA265_RS04025 0.87 +0.3 966,300 + CA265_RS04025 0.88 +0.0 966,308 - CA265_RS04025 0.88 +1.5 966,308 - CA265_RS04025 0.88 -0.0 966,314 - CA265_RS04025 0.89 +0.5 966,370 + +0.9 966,404 - +0.5 966,483 + -0.6 966,529 - +1.4 966,570 + +0.2 966,571 - +0.6 966,602 - +0.6 966,650 + -0.1 966,651 - -1.1 966,654 - -1.2 966,656 - -0.4 966,671 + +0.5 966,672 - +0.2 966,704 + -0.4 966,705 - -0.4 966,737 + -0.6 966,737 + -1.3 966,737 + -0.5 966,756 + +0.2 966,756 + -0.6 966,757 - -0.2 966,789 + -0.1 966,895 + CA265_RS04030 0.11 -0.3 967,039 - CA265_RS04030 0.23 -1.8 967,088 + CA265_RS04030 0.27 +0.9 967,089 - CA265_RS04030 0.27 +0.2 967,123 + CA265_RS04030 0.30 +0.6 967,184 + CA265_RS04030 0.35 -0.6 967,282 + CA265_RS04030 0.43 -1.0 967,282 + CA265_RS04030 0.43 -0.8 967,282 + CA265_RS04030 0.43 -0.6 967,282 + CA265_RS04030 0.43 -0.7 967,294 + CA265_RS04030 0.44 +0.5 967,295 - CA265_RS04030 0.45 +0.0 967,352 - CA265_RS04030 0.49 -1.1 967,367 + CA265_RS04030 0.51 -0.5 967,368 - CA265_RS04030 0.51 +1.0 967,381 + CA265_RS04030 0.52 -0.8 967,381 - CA265_RS04030 0.52 +0.2 967,417 + CA265_RS04030 0.55 +0.2 967,417 + CA265_RS04030 0.55 -0.6 967,418 - CA265_RS04030 0.55 -0.1 967,418 - CA265_RS04030 0.55 +0.7 967,418 - CA265_RS04030 0.55 -0.3 967,421 + CA265_RS04030 0.55 -0.3 967,506 + CA265_RS04030 0.62 -0.1 967,506 + CA265_RS04030 0.62 -0.1 967,506 + CA265_RS04030 0.62 -0.7 967,538 - CA265_RS04030 0.65 +0.1 967,587 - CA265_RS04030 0.69 -0.3 967,597 + CA265_RS04030 0.70 -0.2 967,598 - CA265_RS04030 0.70 +0.0 967,609 - CA265_RS04030 0.71 -0.1 967,675 - CA265_RS04030 0.76 -0.8 967,853 + -0.5 967,853 + -1.0 967,885 - -0.3 967,917 + -0.3 967,959 - +1.0 967,970 + +0.5 968,002 - +0.1 968,080 + -0.4 968,170 + CA265_RS04035 0.12 -0.9 968,171 - CA265_RS04035 0.12 +0.0 968,291 - CA265_RS04035 0.20 -0.3 968,336 + CA265_RS04035 0.23 -0.1 968,336 + CA265_RS04035 0.23 -0.9 968,337 - CA265_RS04035 0.23 -2.0 968,337 - CA265_RS04035 0.23 +0.4 968,370 - CA265_RS04035 0.25 -0.8 968,403 + CA265_RS04035 0.27 -1.9 968,403 + CA265_RS04035 0.27 -0.9 968,404 - CA265_RS04035 0.27 -0.1 968,412 + CA265_RS04035 0.28 -0.2 968,434 + CA265_RS04035 0.29 -0.1 968,434 + CA265_RS04035 0.29 -0.8 968,435 - CA265_RS04035 0.29 -0.1 968,451 - CA265_RS04035 0.30 -1.3 968,457 + CA265_RS04035 0.31 -1.6 968,515 - CA265_RS04035 0.35 -0.6 968,559 - CA265_RS04035 0.38 +0.2 968,561 - CA265_RS04035 0.38 +0.0 968,561 - CA265_RS04035 0.38 -0.2 968,608 + CA265_RS04035 0.41 -1.0 968,677 - CA265_RS04035 0.45 -0.9 968,784 + CA265_RS04035 0.53 -0.4 968,872 + CA265_RS04035 0.58 -1.1 968,872 + CA265_RS04035 0.58 -1.6 968,885 + CA265_RS04035 0.59 -0.2 968,886 - CA265_RS04035 0.59 -0.1 968,886 - CA265_RS04035 0.59 +0.7
Or see this region's nucleotide sequence