Experiment: m.b. nitrate 100 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c20520 and PGA1_c20530 are separated by 83 nucleotides PGA1_c20530 and PGA1_c20535 are separated by 66 nucleotides PGA1_c20535 and PGA1_c20540 are separated by 219 nucleotides
PGA1_c20520: PGA1_c20520 - acetyltransferase and methytransferase domain-containing protein, at 2,125,998 to 2,127,035
_c20520
PGA1_c20530: PGA1_c20530 - putative transcriptional regulator, at 2,127,119 to 2,127,541
_c20530
PGA1_c20535: PGA1_c20535 - hypothetical protein, at 2,127,608 to 2,128,015
_c20535
PGA1_c20540: PGA1_c20540 - ATP-dependent protease La, at 2,128,235 to 2,130,649
_c20540
Position (kb)
2127
2128 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2126.120 kb on + strand, within PGA1_c20520 at 2126.135 kb on + strand, within PGA1_c20520 at 2126.143 kb on - strand, within PGA1_c20520 at 2126.193 kb on + strand, within PGA1_c20520 at 2126.206 kb on - strand, within PGA1_c20520 at 2126.218 kb on + strand, within PGA1_c20520 at 2126.280 kb on - strand, within PGA1_c20520 at 2126.295 kb on - strand, within PGA1_c20520 at 2126.342 kb on + strand, within PGA1_c20520 at 2126.342 kb on + strand, within PGA1_c20520 at 2126.342 kb on + strand, within PGA1_c20520 at 2126.350 kb on - strand, within PGA1_c20520 at 2126.377 kb on + strand, within PGA1_c20520 at 2126.382 kb on + strand, within PGA1_c20520 at 2126.390 kb on - strand, within PGA1_c20520 at 2126.390 kb on - strand, within PGA1_c20520 at 2126.390 kb on - strand, within PGA1_c20520 at 2126.390 kb on - strand, within PGA1_c20520 at 2126.390 kb on - strand, within PGA1_c20520 at 2126.390 kb on - strand, within PGA1_c20520 at 2126.417 kb on - strand, within PGA1_c20520 at 2126.422 kb on - strand, within PGA1_c20520 at 2126.424 kb on - strand, within PGA1_c20520 at 2126.480 kb on + strand, within PGA1_c20520 at 2126.488 kb on + strand, within PGA1_c20520 at 2126.488 kb on + strand, within PGA1_c20520 at 2126.488 kb on + strand, within PGA1_c20520 at 2126.488 kb on - strand, within PGA1_c20520 at 2126.488 kb on - strand, within PGA1_c20520 at 2126.542 kb on + strand, within PGA1_c20520 at 2126.551 kb on - strand, within PGA1_c20520 at 2126.554 kb on + strand, within PGA1_c20520 at 2126.562 kb on - strand, within PGA1_c20520 at 2126.595 kb on - strand, within PGA1_c20520 at 2126.612 kb on + strand, within PGA1_c20520 at 2126.620 kb on - strand, within PGA1_c20520 at 2126.695 kb on + strand, within PGA1_c20520 at 2126.695 kb on + strand, within PGA1_c20520 at 2126.695 kb on + strand, within PGA1_c20520 at 2126.703 kb on - strand, within PGA1_c20520 at 2126.730 kb on + strand, within PGA1_c20520 at 2126.767 kb on - strand, within PGA1_c20520 at 2126.768 kb on - strand, within PGA1_c20520 at 2126.827 kb on - strand, within PGA1_c20520 at 2126.883 kb on + strand, within PGA1_c20520 at 2126.904 kb on + strand, within PGA1_c20520 at 2126.910 kb on + strand, within PGA1_c20520 at 2126.912 kb on - strand, within PGA1_c20520 at 2126.912 kb on - strand, within PGA1_c20520 at 2126.963 kb on + strand at 2126.968 kb on + strand at 2126.968 kb on + strand at 2126.971 kb on - strand at 2126.976 kb on + strand at 2126.976 kb on - strand at 2127.003 kb on - strand at 2127.010 kb on + strand at 2127.068 kb on + strand at 2127.188 kb on + strand, within PGA1_c20530 at 2127.198 kb on + strand, within PGA1_c20530 at 2127.205 kb on + strand, within PGA1_c20530 at 2127.303 kb on - strand, within PGA1_c20530 at 2127.315 kb on + strand, within PGA1_c20530 at 2127.396 kb on + strand, within PGA1_c20530 at 2127.446 kb on - strand, within PGA1_c20530 at 2127.469 kb on + strand, within PGA1_c20530 at 2127.477 kb on - strand, within PGA1_c20530 at 2127.495 kb on + strand, within PGA1_c20530 at 2127.513 kb on + strand at 2127.513 kb on + strand at 2127.521 kb on - strand at 2127.521 kb on - strand at 2127.521 kb on - strand at 2127.618 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on + strand at 2127.626 kb on - strand at 2127.628 kb on + strand at 2127.631 kb on + strand at 2127.634 kb on - strand at 2127.634 kb on - strand at 2127.634 kb on - strand at 2127.634 kb on - strand at 2127.634 kb on - strand at 2127.634 kb on - strand at 2127.634 kb on - strand at 2127.683 kb on - strand, within PGA1_c20535 at 2127.779 kb on + strand, within PGA1_c20535 at 2127.835 kb on - strand, within PGA1_c20535 at 2127.837 kb on - strand, within PGA1_c20535 at 2127.844 kb on - strand, within PGA1_c20535 at 2127.844 kb on - strand, within PGA1_c20535 at 2127.911 kb on - strand, within PGA1_c20535 at 2127.927 kb on + strand, within PGA1_c20535 at 2127.927 kb on + strand, within PGA1_c20535 at 2128.022 kb on - strand at 2128.116 kb on + strand at 2128.116 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 100 mM remove 2,126,120 + PGA1_c20520 0.12 -0.1 2,126,135 + PGA1_c20520 0.13 -0.9 2,126,143 - PGA1_c20520 0.14 +0.4 2,126,193 + PGA1_c20520 0.19 -1.9 2,126,206 - PGA1_c20520 0.20 -0.1 2,126,218 + PGA1_c20520 0.21 +1.1 2,126,280 - PGA1_c20520 0.27 -0.0 2,126,295 - PGA1_c20520 0.29 +0.8 2,126,342 + PGA1_c20520 0.33 -0.7 2,126,342 + PGA1_c20520 0.33 +0.2 2,126,342 + PGA1_c20520 0.33 +0.0 2,126,350 - PGA1_c20520 0.34 +0.9 2,126,377 + PGA1_c20520 0.37 +0.9 2,126,382 + PGA1_c20520 0.37 -0.1 2,126,390 - PGA1_c20520 0.38 -0.7 2,126,390 - PGA1_c20520 0.38 -2.6 2,126,390 - PGA1_c20520 0.38 -1.6 2,126,390 - PGA1_c20520 0.38 -1.1 2,126,390 - PGA1_c20520 0.38 +1.1 2,126,390 - PGA1_c20520 0.38 +0.6 2,126,417 - PGA1_c20520 0.40 +1.7 2,126,422 - PGA1_c20520 0.41 +0.8 2,126,424 - PGA1_c20520 0.41 +0.4 2,126,480 + PGA1_c20520 0.46 -0.6 2,126,488 + PGA1_c20520 0.47 -0.3 2,126,488 + PGA1_c20520 0.47 +0.3 2,126,488 + PGA1_c20520 0.47 +0.7 2,126,488 - PGA1_c20520 0.47 +1.0 2,126,488 - PGA1_c20520 0.47 -0.5 2,126,542 + PGA1_c20520 0.52 -0.4 2,126,551 - PGA1_c20520 0.53 +0.8 2,126,554 + PGA1_c20520 0.54 -0.2 2,126,562 - PGA1_c20520 0.54 -0.3 2,126,595 - PGA1_c20520 0.58 +0.4 2,126,612 + PGA1_c20520 0.59 -0.1 2,126,620 - PGA1_c20520 0.60 +0.3 2,126,695 + PGA1_c20520 0.67 -0.3 2,126,695 + PGA1_c20520 0.67 -0.6 2,126,695 + PGA1_c20520 0.67 -1.6 2,126,703 - PGA1_c20520 0.68 -0.5 2,126,730 + PGA1_c20520 0.71 +2.3 2,126,767 - PGA1_c20520 0.74 +0.0 2,126,768 - PGA1_c20520 0.74 +0.0 2,126,827 - PGA1_c20520 0.80 +0.6 2,126,883 + PGA1_c20520 0.85 -0.3 2,126,904 + PGA1_c20520 0.87 -1.3 2,126,910 + PGA1_c20520 0.88 +0.1 2,126,912 - PGA1_c20520 0.88 +0.6 2,126,912 - PGA1_c20520 0.88 -0.3 2,126,963 + +2.0 2,126,968 + +2.0 2,126,968 + +0.4 2,126,971 - -1.1 2,126,976 + -0.2 2,126,976 - -1.0 2,127,003 - -0.0 2,127,010 + +0.3 2,127,068 + +0.3 2,127,188 + PGA1_c20530 0.16 -1.1 2,127,198 + PGA1_c20530 0.19 -0.4 2,127,205 + PGA1_c20530 0.20 -0.5 2,127,303 - PGA1_c20530 0.43 -0.2 2,127,315 + PGA1_c20530 0.46 -0.1 2,127,396 + PGA1_c20530 0.65 -0.3 2,127,446 - PGA1_c20530 0.77 -1.4 2,127,469 + PGA1_c20530 0.83 -0.0 2,127,477 - PGA1_c20530 0.85 -1.3 2,127,495 + PGA1_c20530 0.89 -0.3 2,127,513 + +2.8 2,127,513 + +0.8 2,127,521 - +0.1 2,127,521 - +1.3 2,127,521 - +0.0 2,127,618 + +0.8 2,127,626 + -1.0 2,127,626 + +0.0 2,127,626 + -1.3 2,127,626 + +0.0 2,127,626 + +0.9 2,127,626 + +1.1 2,127,626 + -0.1 2,127,626 + -0.0 2,127,626 + +1.0 2,127,626 - -0.5 2,127,628 + +0.4 2,127,631 + +0.6 2,127,634 - +0.3 2,127,634 - +1.1 2,127,634 - +1.4 2,127,634 - +1.1 2,127,634 - +0.0 2,127,634 - +0.1 2,127,634 - +0.5 2,127,683 - PGA1_c20535 0.18 +0.1 2,127,779 + PGA1_c20535 0.42 +0.1 2,127,835 - PGA1_c20535 0.56 -0.7 2,127,837 - PGA1_c20535 0.56 -0.1 2,127,844 - PGA1_c20535 0.58 -0.2 2,127,844 - PGA1_c20535 0.58 +0.3 2,127,911 - PGA1_c20535 0.74 -0.1 2,127,927 + PGA1_c20535 0.78 +1.0 2,127,927 + PGA1_c20535 0.78 -0.1 2,128,022 - +0.2 2,128,116 + -0.6 2,128,116 + +0.6
Or see this region's nucleotide sequence