Experiment: m.b. nitrate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_262p00220 and PGA1_262p00230 overlap by 4 nucleotides PGA1_262p00230 and PGA1_262p00240 are separated by 126 nucleotides PGA1_262p00240 and PGA1_262p00250 are separated by 41 nucleotides PGA1_262p00250 and PGA1_262p00260 are separated by 109 nucleotides
PGA1_262p00220: PGA1_262p00220 - putative mandelate racemase/muconate lactonizing enzyme, at 26,417 to 27,592
_262p00220
PGA1_262p00230: PGA1_262p00230 - betaine aldehyde dehydrogenase BetB, at 27,589 to 29,073
_262p00230
PGA1_262p00240: PGA1_262p00240 - Protein of unknown function (DUF1289)., at 29,200 to 29,445
_262p00240
PGA1_262p00250: PGA1_262p00250 - ferritin-like protein, at 29,487 to 29,960
_262p00250
PGA1_262p00260: PGA1_262p00260 - hypothetical protein, at 30,070 to 30,510
_262p00260
Position (kb) 27
28
29
30 Strain fitness (log2 ratio) -2
-1
0
1
2 at 26.594 kb on + strand, within PGA1_262p00220 at 26.629 kb on + strand, within PGA1_262p00220 at 26.655 kb on - strand, within PGA1_262p00220 at 26.826 kb on - strand, within PGA1_262p00220 at 26.928 kb on - strand, within PGA1_262p00220 at 27.002 kb on + strand, within PGA1_262p00220 at 27.002 kb on + strand, within PGA1_262p00220 at 27.010 kb on - strand, within PGA1_262p00220 at 27.010 kb on - strand, within PGA1_262p00220 at 27.010 kb on - strand, within PGA1_262p00220 at 27.010 kb on - strand, within PGA1_262p00220 at 27.123 kb on - strand, within PGA1_262p00220 at 27.144 kb on + strand, within PGA1_262p00220 at 27.159 kb on - strand, within PGA1_262p00220 at 27.193 kb on + strand, within PGA1_262p00220 at 27.251 kb on - strand, within PGA1_262p00220 at 27.333 kb on - strand, within PGA1_262p00220 at 27.390 kb on + strand, within PGA1_262p00220 at 27.390 kb on + strand, within PGA1_262p00220 at 27.390 kb on + strand, within PGA1_262p00220 at 27.390 kb on + strand, within PGA1_262p00220 at 27.398 kb on - strand, within PGA1_262p00220 at 27.400 kb on + strand, within PGA1_262p00220 at 27.419 kb on - strand, within PGA1_262p00220 at 27.419 kb on - strand, within PGA1_262p00220 at 27.447 kb on - strand, within PGA1_262p00220 at 27.447 kb on - strand, within PGA1_262p00220 at 27.523 kb on - strand at 27.530 kb on - strand at 27.563 kb on + strand at 27.576 kb on - strand at 27.676 kb on + strand at 27.730 kb on + strand at 28.180 kb on + strand, within PGA1_262p00230 at 28.312 kb on + strand, within PGA1_262p00230 at 28.334 kb on + strand, within PGA1_262p00230 at 28.336 kb on - strand, within PGA1_262p00230 at 28.352 kb on + strand, within PGA1_262p00230 at 28.366 kb on + strand, within PGA1_262p00230 at 28.373 kb on + strand, within PGA1_262p00230 at 28.438 kb on - strand, within PGA1_262p00230 at 28.639 kb on + strand, within PGA1_262p00230 at 28.698 kb on - strand, within PGA1_262p00230 at 28.743 kb on + strand, within PGA1_262p00230 at 28.818 kb on + strand, within PGA1_262p00230 at 28.913 kb on - strand, within PGA1_262p00230 at 28.913 kb on - strand, within PGA1_262p00230 at 28.942 kb on + strand at 28.943 kb on - strand at 28.964 kb on + strand at 29.088 kb on - strand at 29.153 kb on - strand at 29.174 kb on - strand at 29.230 kb on - strand, within PGA1_262p00240 at 29.230 kb on - strand, within PGA1_262p00240 at 29.230 kb on - strand, within PGA1_262p00240 at 29.250 kb on + strand, within PGA1_262p00240 at 29.348 kb on + strand, within PGA1_262p00240 at 29.380 kb on + strand, within PGA1_262p00240 at 29.484 kb on + strand at 29.484 kb on + strand at 29.484 kb on + strand at 29.492 kb on - strand at 29.492 kb on - strand at 29.632 kb on + strand, within PGA1_262p00250 at 29.733 kb on + strand, within PGA1_262p00250 at 29.897 kb on + strand, within PGA1_262p00250 at 29.901 kb on - strand, within PGA1_262p00250 at 29.906 kb on - strand, within PGA1_262p00250 at 29.954 kb on + strand at 30.013 kb on + strand at 30.022 kb on - strand at 30.032 kb on - strand at 30.047 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 50 mM remove 26,594 + PGA1_262p00220 0.15 -0.2 26,629 + PGA1_262p00220 0.18 +2.3 26,655 - PGA1_262p00220 0.20 -0.3 26,826 - PGA1_262p00220 0.35 -0.0 26,928 - PGA1_262p00220 0.43 -0.6 27,002 + PGA1_262p00220 0.50 -2.1 27,002 + PGA1_262p00220 0.50 +2.1 27,010 - PGA1_262p00220 0.50 +0.0 27,010 - PGA1_262p00220 0.50 -0.4 27,010 - PGA1_262p00220 0.50 +0.5 27,010 - PGA1_262p00220 0.50 -1.4 27,123 - PGA1_262p00220 0.60 -0.6 27,144 + PGA1_262p00220 0.62 -0.2 27,159 - PGA1_262p00220 0.63 +1.8 27,193 + PGA1_262p00220 0.66 +1.6 27,251 - PGA1_262p00220 0.71 +1.2 27,333 - PGA1_262p00220 0.78 -0.5 27,390 + PGA1_262p00220 0.83 +0.5 27,390 + PGA1_262p00220 0.83 -1.8 27,390 + PGA1_262p00220 0.83 -0.6 27,390 + PGA1_262p00220 0.83 +0.2 27,398 - PGA1_262p00220 0.83 -0.5 27,400 + PGA1_262p00220 0.84 -0.8 27,419 - PGA1_262p00220 0.85 +1.2 27,419 - PGA1_262p00220 0.85 +0.7 27,447 - PGA1_262p00220 0.88 +0.2 27,447 - PGA1_262p00220 0.88 -0.1 27,523 - +0.0 27,530 - -1.8 27,563 + +0.6 27,576 - +0.2 27,676 + -1.6 27,730 + -1.3 28,180 + PGA1_262p00230 0.40 +0.2 28,312 + PGA1_262p00230 0.49 +0.2 28,334 + PGA1_262p00230 0.50 +1.2 28,336 - PGA1_262p00230 0.50 -0.5 28,352 + PGA1_262p00230 0.51 +0.4 28,366 + PGA1_262p00230 0.52 +1.5 28,373 + PGA1_262p00230 0.53 -1.4 28,438 - PGA1_262p00230 0.57 +0.4 28,639 + PGA1_262p00230 0.71 -0.2 28,698 - PGA1_262p00230 0.75 -0.6 28,743 + PGA1_262p00230 0.78 +1.0 28,818 + PGA1_262p00230 0.83 +1.6 28,913 - PGA1_262p00230 0.89 -1.0 28,913 - PGA1_262p00230 0.89 -0.1 28,942 + +0.4 28,943 - -0.5 28,964 + -1.2 29,088 - +0.6 29,153 - +0.3 29,174 - +0.2 29,230 - PGA1_262p00240 0.12 +0.8 29,230 - PGA1_262p00240 0.12 +0.8 29,230 - PGA1_262p00240 0.12 +1.6 29,250 + PGA1_262p00240 0.20 +0.8 29,348 + PGA1_262p00240 0.60 -0.7 29,380 + PGA1_262p00240 0.73 +1.5 29,484 + -1.4 29,484 + +1.2 29,484 + -1.2 29,492 - +2.0 29,492 - +0.5 29,632 + PGA1_262p00250 0.31 +0.2 29,733 + PGA1_262p00250 0.52 -0.6 29,897 + PGA1_262p00250 0.86 +1.3 29,901 - PGA1_262p00250 0.87 +1.0 29,906 - PGA1_262p00250 0.88 +1.4 29,954 + -0.6 30,013 + -1.8 30,022 - -1.8 30,032 - -0.8 30,047 + -0.2
Or see this region's nucleotide sequence