Experiment: m.b. nitrate 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c21430 and PGA1_c21440 are separated by 8 nucleotides PGA1_c21440 and PGA1_c21450 are separated by 47 nucleotides PGA1_c21450 and PGA1_c21460 are separated by 71 nucleotides PGA1_c21460 and PGA1_c21470 are separated by 136 nucleotides
PGA1_c21430: PGA1_c21430 - putative permease, at 2,220,788 to 2,221,855
_c21430
PGA1_c21440: PGA1_c21440 - hypothetical protein, at 2,221,864 to 2,222,634
_c21440
PGA1_c21450: PGA1_c21450 - phosphatase-like protein, at 2,222,682 to 2,224,226
_c21450
PGA1_c21460: PGA1_c21460 - hypothetical protein, at 2,224,298 to 2,224,738
_c21460
PGA1_c21470: PGA1_c21470 - endonuclease/exonuclease/phosphatase family protein, at 2,224,875 to 2,225,870
_c21470
Position (kb)
2222
2223
2224
2225 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2221.702 kb on + strand, within PGA1_c21430 at 2221.726 kb on + strand, within PGA1_c21430 at 2221.770 kb on + strand at 2221.770 kb on + strand at 2221.770 kb on + strand at 2221.778 kb on - strand at 2221.805 kb on + strand at 2221.813 kb on - strand at 2221.828 kb on + strand at 2221.836 kb on - strand at 2221.836 kb on - strand at 2221.843 kb on - strand at 2221.862 kb on - strand at 2222.040 kb on + strand, within PGA1_c21440 at 2222.165 kb on - strand, within PGA1_c21440 at 2222.262 kb on + strand, within PGA1_c21440 at 2222.268 kb on + strand, within PGA1_c21440 at 2222.650 kb on + strand at 2222.740 kb on - strand at 2224.270 kb on - strand at 2224.272 kb on + strand at 2224.280 kb on - strand at 2224.464 kb on + strand, within PGA1_c21460 at 2224.578 kb on + strand, within PGA1_c21460 at 2224.591 kb on - strand, within PGA1_c21460 at 2224.593 kb on + strand, within PGA1_c21460 at 2224.596 kb on - strand, within PGA1_c21460 at 2224.603 kb on + strand, within PGA1_c21460 at 2224.611 kb on - strand, within PGA1_c21460 at 2224.616 kb on + strand, within PGA1_c21460 at 2224.624 kb on - strand, within PGA1_c21460 at 2224.696 kb on + strand at 2224.711 kb on - strand at 2224.741 kb on - strand at 2224.748 kb on - strand at 2224.748 kb on - strand at 2224.748 kb on - strand at 2224.772 kb on - strand at 2224.814 kb on + strand at 2224.814 kb on + strand at 2224.817 kb on - strand at 2224.855 kb on - strand at 2224.875 kb on - strand at 2224.886 kb on - strand at 2224.967 kb on - strand at 2224.975 kb on - strand, within PGA1_c21470 at 2225.012 kb on + strand, within PGA1_c21470 at 2225.012 kb on + strand, within PGA1_c21470 at 2225.020 kb on - strand, within PGA1_c21470 at 2225.020 kb on - strand, within PGA1_c21470 at 2225.020 kb on - strand, within PGA1_c21470 at 2225.094 kb on - strand, within PGA1_c21470 at 2225.122 kb on - strand, within PGA1_c21470 at 2225.129 kb on - strand, within PGA1_c21470 at 2225.198 kb on + strand, within PGA1_c21470 at 2225.199 kb on + strand, within PGA1_c21470 at 2225.199 kb on + strand, within PGA1_c21470 at 2225.212 kb on - strand, within PGA1_c21470 at 2225.212 kb on - strand, within PGA1_c21470
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. nitrate 50 mM remove 2,221,702 + PGA1_c21430 0.86 +0.8 2,221,726 + PGA1_c21430 0.88 +0.1 2,221,770 + +1.0 2,221,770 + -1.6 2,221,770 + -1.2 2,221,778 - -1.2 2,221,805 + -0.8 2,221,813 - +0.3 2,221,828 + +0.1 2,221,836 - -0.8 2,221,836 - +0.0 2,221,843 - -1.2 2,221,862 - -1.7 2,222,040 + PGA1_c21440 0.23 -3.2 2,222,165 - PGA1_c21440 0.39 -3.4 2,222,262 + PGA1_c21440 0.52 -2.2 2,222,268 + PGA1_c21440 0.52 -0.6 2,222,650 + +2.7 2,222,740 - +1.2 2,224,270 - +0.1 2,224,272 + +3.2 2,224,280 - +0.9 2,224,464 + PGA1_c21460 0.38 +0.9 2,224,578 + PGA1_c21460 0.63 -0.6 2,224,591 - PGA1_c21460 0.66 -0.8 2,224,593 + PGA1_c21460 0.67 +1.6 2,224,596 - PGA1_c21460 0.68 +1.6 2,224,603 + PGA1_c21460 0.69 -2.2 2,224,611 - PGA1_c21460 0.71 -1.6 2,224,616 + PGA1_c21460 0.72 -0.6 2,224,624 - PGA1_c21460 0.74 +1.7 2,224,696 + -1.2 2,224,711 - +1.4 2,224,741 - +2.0 2,224,748 - -2.5 2,224,748 - +0.6 2,224,748 - +0.4 2,224,772 - +0.6 2,224,814 + +0.8 2,224,814 + +0.2 2,224,817 - +0.7 2,224,855 - +0.1 2,224,875 - +1.2 2,224,886 - -0.6 2,224,967 - +0.1 2,224,975 - PGA1_c21470 0.10 -0.6 2,225,012 + PGA1_c21470 0.14 +0.6 2,225,012 + PGA1_c21470 0.14 -0.6 2,225,020 - PGA1_c21470 0.15 +1.2 2,225,020 - PGA1_c21470 0.15 +0.6 2,225,020 - PGA1_c21470 0.15 +0.2 2,225,094 - PGA1_c21470 0.22 -0.4 2,225,122 - PGA1_c21470 0.25 +0.8 2,225,129 - PGA1_c21470 0.26 +0.6 2,225,198 + PGA1_c21470 0.32 -0.7 2,225,199 + PGA1_c21470 0.33 +2.4 2,225,199 + PGA1_c21470 0.33 -0.6 2,225,212 - PGA1_c21470 0.34 -0.6 2,225,212 - PGA1_c21470 0.34 -0.6
Or see this region's nucleotide sequence