Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0927 and Psest_0928 are separated by 44 nucleotides Psest_0928 and Psest_0929 are separated by 86 nucleotides Psest_0929 and Psest_0930 are separated by 28 nucleotides
Psest_0927: Psest_0927 - zinc-binding alcohol dehydrogenase family protein, at 1,005,057 to 1,006,067
_0927
Psest_0928: Psest_0928 - Gluconolactonase, at 1,006,112 to 1,007,293
_0928
Psest_0929: Psest_0929 - ABC-type proline/glycine betaine transport systems, periplasmic components, at 1,007,380 to 1,008,159
_0929
Psest_0930: Psest_0930 - Nitroreductase, at 1,008,188 to 1,008,841
_0930
Position (kb)
1006
1007
1008 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1005.137 kb on + strand at 1005.224 kb on + strand, within Psest_0927 at 1005.265 kb on + strand, within Psest_0927 at 1005.265 kb on + strand, within Psest_0927 at 1005.265 kb on + strand, within Psest_0927 at 1005.321 kb on + strand, within Psest_0927 at 1005.321 kb on + strand, within Psest_0927 at 1005.321 kb on + strand, within Psest_0927 at 1005.321 kb on + strand, within Psest_0927 at 1005.322 kb on - strand, within Psest_0927 at 1005.322 kb on - strand, within Psest_0927 at 1005.322 kb on - strand, within Psest_0927 at 1005.322 kb on - strand, within Psest_0927 at 1005.322 kb on - strand, within Psest_0927 at 1005.351 kb on + strand, within Psest_0927 at 1005.351 kb on + strand, within Psest_0927 at 1005.351 kb on + strand, within Psest_0927 at 1005.351 kb on + strand, within Psest_0927 at 1005.352 kb on - strand, within Psest_0927 at 1005.352 kb on - strand, within Psest_0927 at 1005.380 kb on + strand, within Psest_0927 at 1005.380 kb on + strand, within Psest_0927 at 1005.380 kb on + strand, within Psest_0927 at 1005.381 kb on - strand, within Psest_0927 at 1005.381 kb on - strand, within Psest_0927 at 1005.381 kb on - strand, within Psest_0927 at 1005.682 kb on + strand, within Psest_0927 at 1005.682 kb on + strand, within Psest_0927 at 1005.682 kb on + strand, within Psest_0927 at 1005.682 kb on + strand, within Psest_0927 at 1005.683 kb on - strand, within Psest_0927 at 1005.741 kb on + strand, within Psest_0927 at 1005.741 kb on + strand, within Psest_0927 at 1005.741 kb on + strand, within Psest_0927 at 1005.741 kb on + strand, within Psest_0927 at 1005.741 kb on + strand, within Psest_0927 at 1005.742 kb on - strand, within Psest_0927 at 1005.742 kb on - strand, within Psest_0927 at 1005.742 kb on - strand, within Psest_0927 at 1005.742 kb on - strand, within Psest_0927 at 1005.742 kb on - strand, within Psest_0927 at 1005.742 kb on - strand, within Psest_0927 at 1005.742 kb on - strand, within Psest_0927 at 1005.742 kb on - strand, within Psest_0927 at 1005.879 kb on + strand, within Psest_0927 at 1005.953 kb on + strand, within Psest_0927 at 1005.953 kb on + strand, within Psest_0927 at 1005.953 kb on + strand, within Psest_0927 at 1005.954 kb on - strand, within Psest_0927 at 1005.954 kb on - strand, within Psest_0927 at 1005.954 kb on - strand, within Psest_0927 at 1005.954 kb on - strand, within Psest_0927 at 1005.954 kb on - strand, within Psest_0927 at 1005.954 kb on - strand, within Psest_0927 at 1006.071 kb on - strand at 1006.071 kb on - strand at 1006.071 kb on - strand at 1006.071 kb on - strand at 1006.245 kb on + strand, within Psest_0928 at 1006.246 kb on - strand, within Psest_0928 at 1006.246 kb on - strand, within Psest_0928 at 1006.301 kb on - strand, within Psest_0928 at 1006.301 kb on - strand, within Psest_0928 at 1006.301 kb on - strand, within Psest_0928 at 1006.610 kb on + strand, within Psest_0928 at 1006.610 kb on + strand, within Psest_0928 at 1006.610 kb on + strand, within Psest_0928 at 1006.610 kb on + strand, within Psest_0928 at 1006.610 kb on + strand, within Psest_0928 at 1006.610 kb on + strand, within Psest_0928 at 1006.610 kb on + strand, within Psest_0928 at 1006.610 kb on + strand, within Psest_0928 at 1006.610 kb on + strand, within Psest_0928 at 1006.610 kb on + strand, within Psest_0928 at 1006.610 kb on + strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.611 kb on - strand, within Psest_0928 at 1006.662 kb on - strand, within Psest_0928 at 1006.892 kb on + strand, within Psest_0928 at 1006.892 kb on + strand, within Psest_0928 at 1006.892 kb on + strand, within Psest_0928 at 1006.892 kb on + strand, within Psest_0928 at 1006.892 kb on + strand, within Psest_0928 at 1006.892 kb on + strand, within Psest_0928 at 1006.892 kb on + strand, within Psest_0928 at 1006.892 kb on + strand, within Psest_0928 at 1006.892 kb on + strand, within Psest_0928 at 1006.893 kb on - strand, within Psest_0928 at 1006.893 kb on - strand, within Psest_0928 at 1006.893 kb on - strand, within Psest_0928 at 1006.893 kb on - strand, within Psest_0928 at 1006.893 kb on - strand, within Psest_0928 at 1006.893 kb on - strand, within Psest_0928 at 1007.061 kb on - strand, within Psest_0928 at 1007.168 kb on + strand, within Psest_0928 at 1007.174 kb on + strand, within Psest_0928 at 1007.174 kb on + strand, within Psest_0928 at 1007.174 kb on + strand, within Psest_0928 at 1007.174 kb on + strand, within Psest_0928 at 1007.174 kb on + strand, within Psest_0928 at 1007.244 kb on - strand at 1007.284 kb on + strand at 1007.285 kb on - strand at 1007.285 kb on - strand at 1007.285 kb on - strand at 1007.285 kb on - strand at 1007.285 kb on - strand at 1007.404 kb on + strand at 1007.404 kb on + strand at 1007.405 kb on - strand at 1007.453 kb on + strand at 1007.453 kb on + strand at 1007.470 kb on + strand, within Psest_0929 at 1007.471 kb on - strand, within Psest_0929 at 1007.471 kb on - strand, within Psest_0929 at 1007.471 kb on - strand, within Psest_0929 at 1007.578 kb on + strand, within Psest_0929 at 1007.578 kb on + strand, within Psest_0929 at 1007.578 kb on + strand, within Psest_0929 at 1007.591 kb on - strand, within Psest_0929 at 1007.623 kb on + strand, within Psest_0929 at 1007.632 kb on + strand, within Psest_0929 at 1007.656 kb on + strand, within Psest_0929 at 1007.656 kb on + strand, within Psest_0929 at 1007.656 kb on + strand, within Psest_0929 at 1007.656 kb on + strand, within Psest_0929 at 1007.656 kb on + strand, within Psest_0929 at 1007.657 kb on - strand, within Psest_0929 at 1007.657 kb on - strand, within Psest_0929 at 1007.657 kb on - strand, within Psest_0929 at 1007.657 kb on - strand, within Psest_0929 at 1007.657 kb on - strand, within Psest_0929 at 1007.676 kb on - strand, within Psest_0929 at 1007.855 kb on - strand, within Psest_0929 at 1008.015 kb on + strand, within Psest_0929 at 1008.016 kb on - strand, within Psest_0929 at 1008.167 kb on - strand at 1008.167 kb on - strand at 1008.219 kb on - strand at 1008.219 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,005,137 + +0.2 1,005,224 + Psest_0927 0.17 -0.2 1,005,265 + Psest_0927 0.21 -0.5 1,005,265 + Psest_0927 0.21 +0.8 1,005,265 + Psest_0927 0.21 +0.1 1,005,321 + Psest_0927 0.26 +2.0 1,005,321 + Psest_0927 0.26 -1.0 1,005,321 + Psest_0927 0.26 +1.5 1,005,321 + Psest_0927 0.26 -1.2 1,005,322 - Psest_0927 0.26 -0.3 1,005,322 - Psest_0927 0.26 -1.7 1,005,322 - Psest_0927 0.26 +0.4 1,005,322 - Psest_0927 0.26 -2.5 1,005,322 - Psest_0927 0.26 -0.6 1,005,351 + Psest_0927 0.29 -1.6 1,005,351 + Psest_0927 0.29 +1.1 1,005,351 + Psest_0927 0.29 -1.1 1,005,351 + Psest_0927 0.29 -1.0 1,005,352 - Psest_0927 0.29 -0.4 1,005,352 - Psest_0927 0.29 -0.3 1,005,380 + Psest_0927 0.32 -0.5 1,005,380 + Psest_0927 0.32 +0.6 1,005,380 + Psest_0927 0.32 +0.0 1,005,381 - Psest_0927 0.32 -0.7 1,005,381 - Psest_0927 0.32 +1.1 1,005,381 - Psest_0927 0.32 +0.6 1,005,682 + Psest_0927 0.62 +0.7 1,005,682 + Psest_0927 0.62 +1.3 1,005,682 + Psest_0927 0.62 +1.7 1,005,682 + Psest_0927 0.62 +1.0 1,005,683 - Psest_0927 0.62 -0.4 1,005,741 + Psest_0927 0.68 +1.2 1,005,741 + Psest_0927 0.68 +0.0 1,005,741 + Psest_0927 0.68 -3.0 1,005,741 + Psest_0927 0.68 -1.8 1,005,741 + Psest_0927 0.68 +1.4 1,005,742 - Psest_0927 0.68 +1.2 1,005,742 - Psest_0927 0.68 -1.2 1,005,742 - Psest_0927 0.68 -2.3 1,005,742 - Psest_0927 0.68 -2.2 1,005,742 - Psest_0927 0.68 +0.2 1,005,742 - Psest_0927 0.68 +1.2 1,005,742 - Psest_0927 0.68 -0.4 1,005,742 - Psest_0927 0.68 +0.4 1,005,879 + Psest_0927 0.81 -2.1 1,005,953 + Psest_0927 0.89 +0.8 1,005,953 + Psest_0927 0.89 -0.9 1,005,953 + Psest_0927 0.89 -0.6 1,005,954 - Psest_0927 0.89 +0.8 1,005,954 - Psest_0927 0.89 +0.1 1,005,954 - Psest_0927 0.89 +0.7 1,005,954 - Psest_0927 0.89 +0.6 1,005,954 - Psest_0927 0.89 -0.0 1,005,954 - Psest_0927 0.89 -0.4 1,006,071 - +0.7 1,006,071 - -0.9 1,006,071 - +1.6 1,006,071 - -0.4 1,006,245 + Psest_0928 0.11 -0.3 1,006,246 - Psest_0928 0.11 +1.0 1,006,246 - Psest_0928 0.11 +0.6 1,006,301 - Psest_0928 0.16 +0.2 1,006,301 - Psest_0928 0.16 +0.3 1,006,301 - Psest_0928 0.16 -0.7 1,006,610 + Psest_0928 0.42 +0.7 1,006,610 + Psest_0928 0.42 +0.4 1,006,610 + Psest_0928 0.42 +0.5 1,006,610 + Psest_0928 0.42 +1.2 1,006,610 + Psest_0928 0.42 -1.2 1,006,610 + Psest_0928 0.42 +0.8 1,006,610 + Psest_0928 0.42 -0.2 1,006,610 + Psest_0928 0.42 +1.2 1,006,610 + Psest_0928 0.42 +1.3 1,006,610 + Psest_0928 0.42 -0.2 1,006,610 + Psest_0928 0.42 +0.2 1,006,611 - Psest_0928 0.42 +0.1 1,006,611 - Psest_0928 0.42 +0.3 1,006,611 - Psest_0928 0.42 +0.5 1,006,611 - Psest_0928 0.42 +0.6 1,006,611 - Psest_0928 0.42 -0.2 1,006,611 - Psest_0928 0.42 +0.1 1,006,611 - Psest_0928 0.42 +0.2 1,006,611 - Psest_0928 0.42 +0.6 1,006,611 - Psest_0928 0.42 +0.6 1,006,611 - Psest_0928 0.42 -0.6 1,006,611 - Psest_0928 0.42 -2.0 1,006,611 - Psest_0928 0.42 +0.3 1,006,611 - Psest_0928 0.42 +1.1 1,006,662 - Psest_0928 0.47 -0.5 1,006,892 + Psest_0928 0.66 +0.3 1,006,892 + Psest_0928 0.66 -0.9 1,006,892 + Psest_0928 0.66 -0.4 1,006,892 + Psest_0928 0.66 -0.2 1,006,892 + Psest_0928 0.66 +0.2 1,006,892 + Psest_0928 0.66 -1.2 1,006,892 + Psest_0928 0.66 +1.0 1,006,892 + Psest_0928 0.66 -0.1 1,006,892 + Psest_0928 0.66 -2.7 1,006,893 - Psest_0928 0.66 +0.1 1,006,893 - Psest_0928 0.66 -0.4 1,006,893 - Psest_0928 0.66 -0.8 1,006,893 - Psest_0928 0.66 +0.7 1,006,893 - Psest_0928 0.66 -1.7 1,006,893 - Psest_0928 0.66 +1.5 1,007,061 - Psest_0928 0.80 +1.3 1,007,168 + Psest_0928 0.89 +0.9 1,007,174 + Psest_0928 0.90 -0.4 1,007,174 + Psest_0928 0.90 +0.1 1,007,174 + Psest_0928 0.90 +0.6 1,007,174 + Psest_0928 0.90 +0.2 1,007,174 + Psest_0928 0.90 +1.2 1,007,244 - -0.9 1,007,284 + +0.8 1,007,285 - +0.5 1,007,285 - +0.3 1,007,285 - +0.4 1,007,285 - +0.5 1,007,285 - +3.6 1,007,404 + -0.2 1,007,404 + +0.5 1,007,405 - -0.1 1,007,453 + -2.1 1,007,453 + +0.3 1,007,470 + Psest_0929 0.12 -0.6 1,007,471 - Psest_0929 0.12 +2.6 1,007,471 - Psest_0929 0.12 +1.2 1,007,471 - Psest_0929 0.12 +0.3 1,007,578 + Psest_0929 0.25 +1.5 1,007,578 + Psest_0929 0.25 -0.6 1,007,578 + Psest_0929 0.25 +0.9 1,007,591 - Psest_0929 0.27 +0.3 1,007,623 + Psest_0929 0.31 +1.4 1,007,632 + Psest_0929 0.32 -0.3 1,007,656 + Psest_0929 0.35 +0.1 1,007,656 + Psest_0929 0.35 +0.7 1,007,656 + Psest_0929 0.35 -1.7 1,007,656 + Psest_0929 0.35 +2.4 1,007,656 + Psest_0929 0.35 -0.2 1,007,657 - Psest_0929 0.36 -1.1 1,007,657 - Psest_0929 0.36 +0.4 1,007,657 - Psest_0929 0.36 +0.5 1,007,657 - Psest_0929 0.36 -0.3 1,007,657 - Psest_0929 0.36 -0.3 1,007,676 - Psest_0929 0.38 +0.6 1,007,855 - Psest_0929 0.61 -0.2 1,008,015 + Psest_0929 0.81 +0.1 1,008,016 - Psest_0929 0.82 -0.2 1,008,167 - +0.2 1,008,167 - +2.6 1,008,219 - +0.5 1,008,219 - -1.6
Or see this region's nucleotide sequence