Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0783 and Psest_0784 are separated by 126 nucleotides Psest_0784 and Psest_0785 are separated by 108 nucleotides
Psest_0783: Psest_0783 - PAS domain S-box, at 852,318 to 856,490
_0783
Psest_0784: Psest_0784 - L-serine dehydratase, iron-sulfur-dependent, single chain form, at 856,617 to 857,993
_0784
Psest_0785: Psest_0785 - Thioredoxin reductase, at 858,102 to 859,049
_0785
Position (kb) 856
857
858 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 855.619 kb on + strand, within Psest_0783 at 855.620 kb on - strand, within Psest_0783 at 855.620 kb on - strand, within Psest_0783 at 855.620 kb on - strand, within Psest_0783 at 855.620 kb on - strand, within Psest_0783 at 855.692 kb on - strand, within Psest_0783 at 855.692 kb on - strand, within Psest_0783 at 855.692 kb on - strand, within Psest_0783 at 855.774 kb on - strand, within Psest_0783 at 856.065 kb on + strand, within Psest_0783 at 856.065 kb on + strand, within Psest_0783 at 856.065 kb on + strand, within Psest_0783 at 856.066 kb on - strand, within Psest_0783 at 856.085 kb on + strand at 856.171 kb on + strand at 856.171 kb on + strand at 856.213 kb on + strand at 856.213 kb on + strand at 856.213 kb on + strand at 856.213 kb on + strand at 856.213 kb on + strand at 856.213 kb on + strand at 856.213 kb on + strand at 856.213 kb on + strand at 856.213 kb on + strand at 856.213 kb on + strand at 856.213 kb on + strand at 856.213 kb on + strand at 856.213 kb on + strand at 856.214 kb on - strand at 856.214 kb on - strand at 856.214 kb on - strand at 856.214 kb on - strand at 856.214 kb on - strand at 856.214 kb on - strand at 856.214 kb on - strand at 856.214 kb on - strand at 856.214 kb on - strand at 856.581 kb on - strand at 856.581 kb on - strand at 856.581 kb on - strand at 856.601 kb on + strand at 856.666 kb on + strand at 856.666 kb on + strand at 856.667 kb on - strand at 856.667 kb on - strand at 856.667 kb on - strand at 856.667 kb on - strand at 856.667 kb on - strand at 857.005 kb on + strand, within Psest_0784 at 857.006 kb on - strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.083 kb on + strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.084 kb on - strand, within Psest_0784 at 857.086 kb on + strand, within Psest_0784 at 857.086 kb on + strand, within Psest_0784 at 857.087 kb on - strand, within Psest_0784 at 857.087 kb on - strand, within Psest_0784 at 857.087 kb on - strand, within Psest_0784 at 857.087 kb on - strand, within Psest_0784 at 857.182 kb on + strand, within Psest_0784 at 857.183 kb on - strand, within Psest_0784 at 857.560 kb on + strand, within Psest_0784 at 857.560 kb on + strand, within Psest_0784 at 857.560 kb on + strand, within Psest_0784 at 857.561 kb on - strand, within Psest_0784 at 857.561 kb on - strand, within Psest_0784 at 857.561 kb on - strand, within Psest_0784 at 857.561 kb on - strand, within Psest_0784 at 857.564 kb on - strand, within Psest_0784 at 857.721 kb on + strand, within Psest_0784 at 857.721 kb on + strand, within Psest_0784 at 857.721 kb on + strand, within Psest_0784 at 857.842 kb on + strand, within Psest_0784 at 857.842 kb on + strand, within Psest_0784 at 858.020 kb on - strand at 858.020 kb on - strand at 858.020 kb on - strand at 858.020 kb on - strand at 858.021 kb on + strand at 858.021 kb on + strand at 858.021 kb on + strand at 858.021 kb on + strand at 858.021 kb on + strand at 858.022 kb on - strand at 858.022 kb on - strand at 858.022 kb on - strand at 858.022 kb on - strand at 858.022 kb on - strand at 858.022 kb on - strand at 858.027 kb on + strand at 858.027 kb on + strand at 858.104 kb on - strand at 858.104 kb on - strand at 858.152 kb on + strand at 858.353 kb on - strand, within Psest_0785 at 858.353 kb on - strand, within Psest_0785 at 858.457 kb on + strand, within Psest_0785 at 858.581 kb on - strand, within Psest_0785 at 858.581 kb on - strand, within Psest_0785 at 858.962 kb on - strand at 858.962 kb on - strand at 858.962 kb on - strand at 858.962 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 855,619 + Psest_0783 0.79 +0.5 855,620 - Psest_0783 0.79 +2.5 855,620 - Psest_0783 0.79 -0.3 855,620 - Psest_0783 0.79 -1.6 855,620 - Psest_0783 0.79 +0.1 855,692 - Psest_0783 0.81 -0.2 855,692 - Psest_0783 0.81 +0.2 855,692 - Psest_0783 0.81 -0.0 855,774 - Psest_0783 0.83 +0.7 856,065 + Psest_0783 0.90 -0.3 856,065 + Psest_0783 0.90 -1.1 856,065 + Psest_0783 0.90 -1.4 856,066 - Psest_0783 0.90 -2.3 856,085 + +1.4 856,171 + +0.3 856,171 + -0.8 856,213 + +0.3 856,213 + -1.9 856,213 + +0.5 856,213 + -2.8 856,213 + -2.1 856,213 + +0.4 856,213 + -0.9 856,213 + -0.7 856,213 + -1.0 856,213 + -2.8 856,213 + +0.5 856,213 + +0.3 856,213 + +0.4 856,214 - +2.1 856,214 - +2.0 856,214 - +0.1 856,214 - +0.1 856,214 - -0.4 856,214 - +0.2 856,214 - -0.3 856,214 - -0.2 856,214 - +0.5 856,581 - -1.3 856,581 - -1.5 856,581 - -0.2 856,601 + +0.0 856,666 + -0.1 856,666 + -0.2 856,667 - +0.5 856,667 - +1.0 856,667 - +1.3 856,667 - +0.6 856,667 - +0.6 857,005 + Psest_0784 0.28 +2.0 857,006 - Psest_0784 0.28 +0.3 857,083 + Psest_0784 0.34 +0.8 857,083 + Psest_0784 0.34 -1.3 857,083 + Psest_0784 0.34 -2.0 857,083 + Psest_0784 0.34 -1.1 857,083 + Psest_0784 0.34 +1.0 857,083 + Psest_0784 0.34 +2.1 857,083 + Psest_0784 0.34 -1.8 857,083 + Psest_0784 0.34 -0.6 857,083 + Psest_0784 0.34 +0.6 857,083 + Psest_0784 0.34 -0.7 857,083 + Psest_0784 0.34 -2.3 857,083 + Psest_0784 0.34 -1.5 857,083 + Psest_0784 0.34 -2.4 857,083 + Psest_0784 0.34 -3.1 857,083 + Psest_0784 0.34 +0.0 857,083 + Psest_0784 0.34 +0.7 857,083 + Psest_0784 0.34 -1.9 857,083 + Psest_0784 0.34 -1.6 857,083 + Psest_0784 0.34 -0.3 857,083 + Psest_0784 0.34 -0.1 857,083 + Psest_0784 0.34 +0.5 857,084 - Psest_0784 0.34 +0.5 857,084 - Psest_0784 0.34 -1.8 857,084 - Psest_0784 0.34 +0.2 857,084 - Psest_0784 0.34 +0.8 857,084 - Psest_0784 0.34 -1.1 857,084 - Psest_0784 0.34 -0.4 857,084 - Psest_0784 0.34 +0.1 857,084 - Psest_0784 0.34 -0.0 857,084 - Psest_0784 0.34 +1.6 857,084 - Psest_0784 0.34 -2.5 857,084 - Psest_0784 0.34 +1.5 857,084 - Psest_0784 0.34 +1.7 857,084 - Psest_0784 0.34 +0.8 857,084 - Psest_0784 0.34 +0.3 857,084 - Psest_0784 0.34 -0.1 857,084 - Psest_0784 0.34 -1.3 857,084 - Psest_0784 0.34 +1.1 857,084 - Psest_0784 0.34 -0.6 857,086 + Psest_0784 0.34 -0.1 857,086 + Psest_0784 0.34 -1.8 857,087 - Psest_0784 0.34 +1.2 857,087 - Psest_0784 0.34 -2.5 857,087 - Psest_0784 0.34 -2.6 857,087 - Psest_0784 0.34 +0.1 857,182 + Psest_0784 0.41 +0.1 857,183 - Psest_0784 0.41 +1.0 857,560 + Psest_0784 0.68 -1.5 857,560 + Psest_0784 0.68 -1.0 857,560 + Psest_0784 0.68 +0.1 857,561 - Psest_0784 0.69 -0.1 857,561 - Psest_0784 0.69 +0.7 857,561 - Psest_0784 0.69 -0.5 857,561 - Psest_0784 0.69 -0.6 857,564 - Psest_0784 0.69 +0.6 857,721 + Psest_0784 0.80 +0.1 857,721 + Psest_0784 0.80 +0.2 857,721 + Psest_0784 0.80 +1.0 857,842 + Psest_0784 0.89 +0.7 857,842 + Psest_0784 0.89 -2.2 858,020 - -0.6 858,020 - -0.0 858,020 - +2.0 858,020 - -0.8 858,021 + +1.7 858,021 + +0.0 858,021 + +0.4 858,021 + -3.0 858,021 + +3.3 858,022 - -0.6 858,022 - -0.5 858,022 - -2.0 858,022 - +0.8 858,022 - -0.3 858,022 - -0.3 858,027 + +0.2 858,027 + -0.0 858,104 - -1.4 858,104 - -0.6 858,152 + +0.5 858,353 - Psest_0785 0.26 +0.4 858,353 - Psest_0785 0.26 +0.6 858,457 + Psest_0785 0.37 +0.1 858,581 - Psest_0785 0.51 +0.5 858,581 - Psest_0785 0.51 -0.4 858,962 - +1.6 858,962 - +4.3 858,962 - -0.2 858,962 - -1.0
Or see this region's nucleotide sequence