Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0778 and Psest_0779 are separated by 195 nucleotides Psest_0779 and Psest_0780 overlap by 1 nucleotides Psest_0780 and Psest_0781 are separated by 227 nucleotides
Psest_0778: Psest_0778 - Protein involved in biosynthesis of mitomycin antibiotics/polyketide fumonisin, at 846,878 to 847,639
_0778
Psest_0779: Psest_0779 - tyrosine aminotransferase (EC 2.6.1.57) <small>(from data)</small>, at 847,835 to 849,031
_0779
Psest_0780: Psest_0780 - Pterin-4a-carbinolamine dehydratase, at 849,031 to 849,387
_0780
Psest_0781: Psest_0781 - phenylalanine-4-hydroxylase, monomeric form, at 849,615 to 850,400
_0781
Position (kb) 847
848
849
850 Strain fitness (log2 ratio) -2
-1
0
1
2 at 846.885 kb on + strand at 846.885 kb on + strand at 846.973 kb on - strand, within Psest_0778 at 846.973 kb on - strand, within Psest_0778 at 847.020 kb on - strand, within Psest_0778 at 847.230 kb on + strand, within Psest_0778 at 847.230 kb on + strand, within Psest_0778 at 847.230 kb on + strand, within Psest_0778 at 847.231 kb on - strand, within Psest_0778 at 847.375 kb on + strand, within Psest_0778 at 847.671 kb on + strand at 847.671 kb on + strand at 847.671 kb on + strand at 847.671 kb on + strand at 847.671 kb on + strand at 847.672 kb on - strand at 847.672 kb on - strand at 847.672 kb on - strand at 847.836 kb on + strand at 847.836 kb on + strand at 847.837 kb on - strand at 847.929 kb on + strand at 847.929 kb on + strand at 847.929 kb on + strand at 847.929 kb on + strand at 847.929 kb on + strand at 847.930 kb on - strand at 847.959 kb on - strand, within Psest_0779 at 847.983 kb on + strand, within Psest_0779 at 847.983 kb on + strand, within Psest_0779 at 847.983 kb on + strand, within Psest_0779 at 847.984 kb on - strand, within Psest_0779 at 848.584 kb on - strand, within Psest_0779 at 848.584 kb on - strand, within Psest_0779 at 848.835 kb on + strand, within Psest_0779 at 848.835 kb on + strand, within Psest_0779 at 848.836 kb on - strand, within Psest_0779 at 849.032 kb on + strand at 849.032 kb on + strand at 849.032 kb on + strand at 849.033 kb on - strand at 849.422 kb on + strand at 849.423 kb on - strand at 849.423 kb on - strand at 849.423 kb on - strand at 849.458 kb on - strand at 849.480 kb on + strand at 849.480 kb on + strand at 849.481 kb on - strand at 849.524 kb on + strand at 849.524 kb on + strand at 849.524 kb on + strand at 849.524 kb on + strand at 849.524 kb on + strand at 849.525 kb on - strand at 849.525 kb on - strand at 849.616 kb on + strand at 849.736 kb on + strand, within Psest_0781 at 849.736 kb on + strand, within Psest_0781 at 849.736 kb on + strand, within Psest_0781 at 849.736 kb on + strand, within Psest_0781 at 849.736 kb on + strand, within Psest_0781 at 849.737 kb on - strand, within Psest_0781 at 849.737 kb on - strand, within Psest_0781 at 849.737 kb on - strand, within Psest_0781 at 849.737 kb on - strand, within Psest_0781 at 849.764 kb on - strand, within Psest_0781 at 849.826 kb on + strand, within Psest_0781 at 849.826 kb on + strand, within Psest_0781 at 849.826 kb on + strand, within Psest_0781 at 849.826 kb on + strand, within Psest_0781 at 849.827 kb on - strand, within Psest_0781 at 849.827 kb on - strand, within Psest_0781 at 849.865 kb on + strand, within Psest_0781 at 849.865 kb on + strand, within Psest_0781 at 849.934 kb on + strand, within Psest_0781 at 849.934 kb on + strand, within Psest_0781 at 849.935 kb on - strand, within Psest_0781 at 849.935 kb on - strand, within Psest_0781 at 849.935 kb on - strand, within Psest_0781 at 849.977 kb on - strand, within Psest_0781
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 846,885 + +1.2 846,885 + -1.0 846,973 - Psest_0778 0.12 +0.7 846,973 - Psest_0778 0.12 +0.8 847,020 - Psest_0778 0.19 -0.8 847,230 + Psest_0778 0.46 +1.7 847,230 + Psest_0778 0.46 +1.2 847,230 + Psest_0778 0.46 +0.7 847,231 - Psest_0778 0.46 -0.4 847,375 + Psest_0778 0.65 -1.3 847,671 + -1.6 847,671 + -0.9 847,671 + +0.7 847,671 + -1.6 847,671 + +0.1 847,672 - +0.3 847,672 - -0.5 847,672 - +0.4 847,836 + -0.3 847,836 + -1.3 847,837 - +1.8 847,929 + +2.1 847,929 + +0.5 847,929 + +0.4 847,929 + +0.7 847,929 + +1.2 847,930 - -1.3 847,959 - Psest_0779 0.10 -1.3 847,983 + Psest_0779 0.12 -1.9 847,983 + Psest_0779 0.12 -1.1 847,983 + Psest_0779 0.12 +0.2 847,984 - Psest_0779 0.12 +1.3 848,584 - Psest_0779 0.63 -1.1 848,584 - Psest_0779 0.63 -0.6 848,835 + Psest_0779 0.84 +1.1 848,835 + Psest_0779 0.84 -1.3 848,836 - Psest_0779 0.84 +0.7 849,032 + +0.7 849,032 + +2.2 849,032 + -0.5 849,033 - +0.1 849,422 + +1.4 849,423 - +1.7 849,423 - -0.9 849,423 - +0.7 849,458 - -1.1 849,480 + -1.5 849,480 + +0.5 849,481 - +0.4 849,524 + -1.8 849,524 + +0.8 849,524 + -0.5 849,524 + +0.0 849,524 + -0.6 849,525 - +0.1 849,525 - -0.3 849,616 + +0.6 849,736 + Psest_0781 0.15 -1.4 849,736 + Psest_0781 0.15 +0.3 849,736 + Psest_0781 0.15 -0.2 849,736 + Psest_0781 0.15 -0.3 849,736 + Psest_0781 0.15 -0.3 849,737 - Psest_0781 0.16 -0.9 849,737 - Psest_0781 0.16 +1.0 849,737 - Psest_0781 0.16 +0.7 849,737 - Psest_0781 0.16 +0.2 849,764 - Psest_0781 0.19 +1.9 849,826 + Psest_0781 0.27 -1.0 849,826 + Psest_0781 0.27 -0.6 849,826 + Psest_0781 0.27 -1.8 849,826 + Psest_0781 0.27 -2.1 849,827 - Psest_0781 0.27 +1.1 849,827 - Psest_0781 0.27 +0.1 849,865 + Psest_0781 0.32 -0.5 849,865 + Psest_0781 0.32 -1.1 849,934 + Psest_0781 0.41 +0.4 849,934 + Psest_0781 0.41 -0.8 849,935 - Psest_0781 0.41 +0.5 849,935 - Psest_0781 0.41 +0.7 849,935 - Psest_0781 0.41 +1.7 849,977 - Psest_0781 0.46 -1.1
Or see this region's nucleotide sequence