Strain Fitness in Pseudomonas stutzeri RCH2 around Psest_0667

Experiment: L-Isoleucine (N)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPsest_0666 and Psest_0667 are separated by 147 nucleotidesPsest_0667 and Psest_0668 are separated by 6 nucleotidesPsest_0668 and Psest_0669 are separated by 85 nucleotides Psest_0666: Psest_0666 - Uncharacterized protein conserved in bacteria, at 725,370 to 726,182 _0666 Psest_0667: Psest_0667 - Phosphodiesterase/alkaline phosphatase D, at 726,330 to 727,883 _0667 Psest_0668: Psest_0668 - Ethanolamine ammonia-lyase, small subunit, at 727,890 to 728,684 _0668 Psest_0669: Psest_0669 - Ethanolamine ammonia-lyase, large subunit, at 728,770 to 730,161 _0669 Position (kb) 726 727 728Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 725.371 kb on + strandat 725.372 kb on - strandat 725.461 kb on + strand, within Psest_0666at 725.462 kb on - strand, within Psest_0666at 725.475 kb on + strand, within Psest_0666at 725.475 kb on + strand, within Psest_0666at 725.476 kb on - strand, within Psest_0666at 725.476 kb on - strand, within Psest_0666at 725.509 kb on + strand, within Psest_0666at 725.509 kb on + strand, within Psest_0666at 725.510 kb on - strand, within Psest_0666at 725.614 kb on + strand, within Psest_0666at 725.737 kb on + strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 725.738 kb on - strand, within Psest_0666at 726.033 kb on + strand, within Psest_0666at 726.033 kb on + strand, within Psest_0666at 726.033 kb on + strand, within Psest_0666at 726.033 kb on + strand, within Psest_0666at 726.033 kb on + strand, within Psest_0666at 726.034 kb on - strand, within Psest_0666at 726.034 kb on - strand, within Psest_0666at 726.216 kb on + strandat 726.216 kb on + strandat 726.217 kb on - strandat 726.217 kb on - strandat 726.217 kb on - strandat 726.217 kb on - strandat 726.217 kb on - strandat 726.250 kb on + strandat 726.251 kb on - strandat 726.293 kb on - strandat 726.822 kb on + strand, within Psest_0667at 726.822 kb on + strand, within Psest_0667at 726.822 kb on + strand, within Psest_0667at 726.823 kb on - strand, within Psest_0667at 726.823 kb on - strand, within Psest_0667at 726.823 kb on - strand, within Psest_0667at 726.823 kb on - strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.879 kb on + strand, within Psest_0667at 726.880 kb on - strand, within Psest_0667at 726.880 kb on - strand, within Psest_0667at 726.880 kb on - strand, within Psest_0667at 726.996 kb on + strand, within Psest_0667at 726.996 kb on + strand, within Psest_0667at 726.997 kb on - strand, within Psest_0667at 727.002 kb on + strand, within Psest_0667at 727.224 kb on + strand, within Psest_0667at 727.225 kb on - strand, within Psest_0667at 727.239 kb on + strand, within Psest_0667at 727.239 kb on + strand, within Psest_0667at 727.302 kb on - strand, within Psest_0667at 727.334 kb on - strand, within Psest_0667at 727.334 kb on - strand, within Psest_0667at 727.334 kb on - strand, within Psest_0667at 727.334 kb on - strand, within Psest_0667at 727.334 kb on - strand, within Psest_0667at 727.579 kb on - strand, within Psest_0667at 727.593 kb on + strand, within Psest_0667at 727.594 kb on - strand, within Psest_0667at 727.594 kb on - strand, within Psest_0667at 727.596 kb on + strand, within Psest_0667at 727.596 kb on + strand, within Psest_0667at 727.597 kb on - strand, within Psest_0667at 727.597 kb on - strand, within Psest_0667at 727.597 kb on - strand, within Psest_0667at 727.617 kb on + strand, within Psest_0667at 727.617 kb on + strand, within Psest_0667at 727.817 kb on - strandat 727.817 kb on - strandat 727.849 kb on - strandat 727.938 kb on + strandat 727.962 kb on + strandat 728.002 kb on + strand, within Psest_0668at 728.002 kb on + strand, within Psest_0668at 728.029 kb on + strand, within Psest_0668at 728.029 kb on + strand, within Psest_0668at 728.029 kb on + strand, within Psest_0668at 728.029 kb on + strand, within Psest_0668at 728.029 kb on + strand, within Psest_0668at 728.029 kb on + strand, within Psest_0668at 728.029 kb on + strand, within Psest_0668at 728.030 kb on - strand, within Psest_0668at 728.030 kb on - strand, within Psest_0668at 728.030 kb on - strand, within Psest_0668at 728.110 kb on + strand, within Psest_0668at 728.110 kb on + strand, within Psest_0668at 728.110 kb on + strand, within Psest_0668at 728.110 kb on + strand, within Psest_0668at 728.111 kb on - strand, within Psest_0668at 728.111 kb on - strand, within Psest_0668at 728.111 kb on - strand, within Psest_0668at 728.111 kb on - strand, within Psest_0668at 728.111 kb on - strand, within Psest_0668at 728.434 kb on + strand, within Psest_0668at 728.434 kb on + strand, within Psest_0668at 728.434 kb on + strand, within Psest_0668at 728.434 kb on + strand, within Psest_0668at 728.435 kb on - strandat 728.483 kb on + strand, within Psest_0668at 728.483 kb on + strand, within Psest_0668at 728.483 kb on + strand, within Psest_0668at 728.484 kb on - strand, within Psest_0668at 728.484 kb on - strand, within Psest_0668at 728.688 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Isoleucine (N)
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725,371 + -1.3
725,372 - -0.0
725,461 + Psest_0666 0.11 -0.6
725,462 - Psest_0666 0.11 +0.2
725,475 + Psest_0666 0.13 -0.2
725,475 + Psest_0666 0.13 -0.6
725,476 - Psest_0666 0.13 -1.5
725,476 - Psest_0666 0.13 -3.4
725,509 + Psest_0666 0.17 +0.5
725,509 + Psest_0666 0.17 -1.0
725,510 - Psest_0666 0.17 +0.1
725,614 + Psest_0666 0.30 +1.7
725,737 + Psest_0666 0.45 -3.2
725,738 - Psest_0666 0.45 -0.9
725,738 - Psest_0666 0.45 +0.9
725,738 - Psest_0666 0.45 -0.6
725,738 - Psest_0666 0.45 -1.5
725,738 - Psest_0666 0.45 -0.8
725,738 - Psest_0666 0.45 -0.6
725,738 - Psest_0666 0.45 -0.1
726,033 + Psest_0666 0.82 -1.5
726,033 + Psest_0666 0.82 +0.3
726,033 + Psest_0666 0.82 -0.6
726,033 + Psest_0666 0.82 -1.7
726,033 + Psest_0666 0.82 -1.1
726,034 - Psest_0666 0.82 +0.4
726,034 - Psest_0666 0.82 +0.4
726,216 + -1.5
726,216 + -2.1
726,217 - +0.7
726,217 - +1.0
726,217 - -2.2
726,217 - +3.8
726,217 - +1.1
726,250 + -2.1
726,251 - -0.8
726,293 - -0.1
726,822 + Psest_0667 0.32 +2.3
726,822 + Psest_0667 0.32 +1.3
726,822 + Psest_0667 0.32 -0.9
726,823 - Psest_0667 0.32 +0.5
726,823 - Psest_0667 0.32 +0.7
726,823 - Psest_0667 0.32 -1.3
726,823 - Psest_0667 0.32 -1.3
726,879 + Psest_0667 0.35 +0.7
726,879 + Psest_0667 0.35 -0.9
726,879 + Psest_0667 0.35 +0.9
726,879 + Psest_0667 0.35 -1.6
726,879 + Psest_0667 0.35 -0.8
726,879 + Psest_0667 0.35 +0.7
726,879 + Psest_0667 0.35 -1.1
726,879 + Psest_0667 0.35 -1.3
726,880 - Psest_0667 0.35 +0.7
726,880 - Psest_0667 0.35 -1.5
726,880 - Psest_0667 0.35 -0.7
726,996 + Psest_0667 0.43 -0.3
726,996 + Psest_0667 0.43 -1.4
726,997 - Psest_0667 0.43 -1.5
727,002 + Psest_0667 0.43 -0.1
727,224 + Psest_0667 0.58 +3.0
727,225 - Psest_0667 0.58 -0.3
727,239 + Psest_0667 0.58 -0.1
727,239 + Psest_0667 0.58 -1.9
727,302 - Psest_0667 0.63 +2.4
727,334 - Psest_0667 0.65 -2.5
727,334 - Psest_0667 0.65 -2.7
727,334 - Psest_0667 0.65 -2.3
727,334 - Psest_0667 0.65 -0.4
727,334 - Psest_0667 0.65 -1.3
727,579 - Psest_0667 0.80 -1.4
727,593 + Psest_0667 0.81 -0.4
727,594 - Psest_0667 0.81 -1.7
727,594 - Psest_0667 0.81 -0.1
727,596 + Psest_0667 0.81 +0.0
727,596 + Psest_0667 0.81 +1.5
727,597 - Psest_0667 0.82 +1.0
727,597 - Psest_0667 0.82 -2.3
727,597 - Psest_0667 0.82 +0.2
727,617 + Psest_0667 0.83 -0.9
727,617 + Psest_0667 0.83 -0.3
727,817 - -3.2
727,817 - +2.2
727,849 - -0.3
727,938 + -2.1
727,962 + -1.9
728,002 + Psest_0668 0.14 -1.9
728,002 + Psest_0668 0.14 -1.7
728,029 + Psest_0668 0.17 -1.1
728,029 + Psest_0668 0.17 -1.1
728,029 + Psest_0668 0.17 -1.3
728,029 + Psest_0668 0.17 -0.4
728,029 + Psest_0668 0.17 -2.1
728,029 + Psest_0668 0.17 -1.9
728,029 + Psest_0668 0.17 -0.7
728,030 - Psest_0668 0.18 +2.0
728,030 - Psest_0668 0.18 -1.3
728,030 - Psest_0668 0.18 -0.4
728,110 + Psest_0668 0.28 -0.3
728,110 + Psest_0668 0.28 +4.1
728,110 + Psest_0668 0.28 -1.1
728,110 + Psest_0668 0.28 -0.7
728,111 - Psest_0668 0.28 -1.7
728,111 - Psest_0668 0.28 -1.3
728,111 - Psest_0668 0.28 -1.3
728,111 - Psest_0668 0.28 -2.2
728,111 - Psest_0668 0.28 -2.6
728,434 + Psest_0668 0.68 -0.1
728,434 + Psest_0668 0.68 -2.1
728,434 + Psest_0668 0.68 -1.2
728,434 + Psest_0668 0.68 +1.0
728,435 - +3.7
728,483 + Psest_0668 0.75 -0.6
728,483 + Psest_0668 0.75 +1.9
728,483 + Psest_0668 0.75 +1.4
728,484 - Psest_0668 0.75 +1.0
728,484 - Psest_0668 0.75 +1.4
728,688 + -1.1

Or see this region's nucleotide sequence