Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0663 and Psest_0664 are separated by 143 nucleotides Psest_0664 and Psest_0665 are separated by 84 nucleotides Psest_0665 and Psest_0666 are separated by 105 nucleotides
Psest_0663: Psest_0663 - PAS fold., at 721,219 to 723,018
_0663
Psest_0664: Psest_0664 - diguanylate cyclase (GGDEF) domain, at 723,162 to 724,652
_0664
Psest_0665: Psest_0665 - Inorganic pyrophosphatase, at 724,737 to 725,264
_0665
Psest_0666: Psest_0666 - Uncharacterized protein conserved in bacteria, at 725,370 to 726,182
_0666
Position (kb) 723
724
725 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 722.176 kb on + strand, within Psest_0663 at 722.176 kb on + strand, within Psest_0663 at 722.176 kb on + strand, within Psest_0663 at 722.176 kb on + strand, within Psest_0663 at 722.177 kb on - strand, within Psest_0663 at 722.177 kb on - strand, within Psest_0663 at 722.177 kb on - strand, within Psest_0663 at 722.401 kb on - strand, within Psest_0663 at 722.567 kb on + strand, within Psest_0663 at 722.626 kb on - strand, within Psest_0663 at 722.666 kb on + strand, within Psest_0663 at 722.761 kb on + strand, within Psest_0663 at 722.872 kb on + strand at 722.905 kb on + strand at 722.906 kb on - strand at 723.024 kb on + strand at 723.197 kb on - strand at 723.237 kb on - strand at 723.427 kb on - strand, within Psest_0664 at 723.601 kb on + strand, within Psest_0664 at 723.601 kb on + strand, within Psest_0664 at 723.602 kb on - strand, within Psest_0664 at 723.702 kb on + strand, within Psest_0664 at 723.703 kb on - strand, within Psest_0664 at 723.703 kb on - strand, within Psest_0664 at 723.703 kb on - strand, within Psest_0664 at 723.703 kb on - strand, within Psest_0664 at 723.837 kb on + strand, within Psest_0664 at 723.838 kb on - strand, within Psest_0664 at 723.838 kb on - strand, within Psest_0664 at 723.870 kb on + strand, within Psest_0664 at 723.870 kb on + strand, within Psest_0664 at 723.870 kb on + strand, within Psest_0664 at 723.871 kb on - strand, within Psest_0664 at 723.871 kb on - strand, within Psest_0664 at 723.871 kb on - strand, within Psest_0664 at 723.947 kb on - strand, within Psest_0664 at 724.490 kb on - strand, within Psest_0664 at 724.548 kb on + strand at 724.548 kb on + strand at 724.549 kb on - strand at 724.576 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.578 kb on + strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.579 kb on - strand at 724.611 kb on + strand at 724.611 kb on + strand at 724.738 kb on + strand at 724.738 kb on + strand at 724.738 kb on + strand at 724.739 kb on - strand at 724.739 kb on - strand at 725.326 kb on - strand at 725.371 kb on + strand at 725.372 kb on - strand at 725.461 kb on + strand, within Psest_0666 at 725.462 kb on - strand, within Psest_0666 at 725.475 kb on + strand, within Psest_0666 at 725.475 kb on + strand, within Psest_0666 at 725.476 kb on - strand, within Psest_0666 at 725.476 kb on - strand, within Psest_0666 at 725.509 kb on + strand, within Psest_0666 at 725.509 kb on + strand, within Psest_0666 at 725.510 kb on - strand, within Psest_0666 at 725.614 kb on + strand, within Psest_0666
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 722,176 + Psest_0663 0.53 -1.0 722,176 + Psest_0663 0.53 -0.8 722,176 + Psest_0663 0.53 -0.2 722,176 + Psest_0663 0.53 -0.6 722,177 - Psest_0663 0.53 -1.7 722,177 - Psest_0663 0.53 +0.0 722,177 - Psest_0663 0.53 -0.5 722,401 - Psest_0663 0.66 -2.1 722,567 + Psest_0663 0.75 -0.3 722,626 - Psest_0663 0.78 -0.6 722,666 + Psest_0663 0.80 -1.7 722,761 + Psest_0663 0.86 -1.6 722,872 + -0.1 722,905 + -1.1 722,906 - -1.9 723,024 + -1.3 723,197 - -1.1 723,237 - -1.5 723,427 - Psest_0664 0.18 -0.8 723,601 + Psest_0664 0.29 -0.9 723,601 + Psest_0664 0.29 -1.2 723,602 - Psest_0664 0.30 -2.1 723,702 + Psest_0664 0.36 -2.1 723,703 - Psest_0664 0.36 +1.4 723,703 - Psest_0664 0.36 +0.3 723,703 - Psest_0664 0.36 +1.4 723,703 - Psest_0664 0.36 -0.5 723,837 + Psest_0664 0.45 -0.8 723,838 - Psest_0664 0.45 -0.6 723,838 - Psest_0664 0.45 +0.0 723,870 + Psest_0664 0.47 -1.5 723,870 + Psest_0664 0.47 -1.3 723,870 + Psest_0664 0.47 +0.5 723,871 - Psest_0664 0.48 -0.9 723,871 - Psest_0664 0.48 -2.0 723,871 - Psest_0664 0.48 -1.0 723,947 - Psest_0664 0.53 -1.3 724,490 - Psest_0664 0.89 -0.7 724,548 + -2.1 724,548 + +0.1 724,549 - -1.4 724,576 + -1.5 724,578 + -0.5 724,578 + -1.1 724,578 + +3.7 724,578 + -0.4 724,578 + -0.3 724,579 - +0.4 724,579 - +0.3 724,579 - -1.4 724,579 - -2.2 724,579 - -0.0 724,611 + -1.0 724,611 + +0.1 724,738 + -0.3 724,738 + -0.2 724,738 + -1.3 724,739 - -0.4 724,739 - -1.7 725,326 - -1.8 725,371 + -1.3 725,372 - -0.0 725,461 + Psest_0666 0.11 -0.6 725,462 - Psest_0666 0.11 +0.2 725,475 + Psest_0666 0.13 -0.2 725,475 + Psest_0666 0.13 -0.6 725,476 - Psest_0666 0.13 -1.5 725,476 - Psest_0666 0.13 -3.4 725,509 + Psest_0666 0.17 +0.5 725,509 + Psest_0666 0.17 -1.0 725,510 - Psest_0666 0.17 +0.1 725,614 + Psest_0666 0.30 +1.7
Or see this region's nucleotide sequence