Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0661 and Psest_0662 are separated by 16 nucleotides Psest_0662 and Psest_0663 are separated by 72 nucleotides Psest_0663 and Psest_0664 are separated by 143 nucleotides
Psest_0661: Psest_0661 - PAS domain S-box, at 718,437 to 720,476
_0661
Psest_0662: Psest_0662 - Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain, at 720,493 to 721,146
_0662
Psest_0663: Psest_0663 - PAS fold., at 721,219 to 723,018
_0663
Psest_0664: Psest_0664 - diguanylate cyclase (GGDEF) domain, at 723,162 to 724,652
_0664
Position (kb) 721
722
723
724 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 720.712 kb on + strand, within Psest_0662 at 720.760 kb on + strand, within Psest_0662 at 720.760 kb on + strand, within Psest_0662 at 720.761 kb on - strand, within Psest_0662 at 721.091 kb on + strand at 721.255 kb on + strand at 721.256 kb on - strand at 721.343 kb on + strand at 721.619 kb on + strand, within Psest_0663 at 721.619 kb on + strand, within Psest_0663 at 721.620 kb on - strand, within Psest_0663 at 721.620 kb on - strand, within Psest_0663 at 721.620 kb on - strand, within Psest_0663 at 721.620 kb on - strand, within Psest_0663 at 721.845 kb on + strand, within Psest_0663 at 722.176 kb on + strand, within Psest_0663 at 722.176 kb on + strand, within Psest_0663 at 722.176 kb on + strand, within Psest_0663 at 722.176 kb on + strand, within Psest_0663 at 722.177 kb on - strand, within Psest_0663 at 722.177 kb on - strand, within Psest_0663 at 722.177 kb on - strand, within Psest_0663 at 722.401 kb on - strand, within Psest_0663 at 722.567 kb on + strand, within Psest_0663 at 722.626 kb on - strand, within Psest_0663 at 722.666 kb on + strand, within Psest_0663 at 722.761 kb on + strand, within Psest_0663 at 722.872 kb on + strand at 722.905 kb on + strand at 722.906 kb on - strand at 723.024 kb on + strand at 723.197 kb on - strand at 723.237 kb on - strand at 723.427 kb on - strand, within Psest_0664 at 723.601 kb on + strand, within Psest_0664 at 723.601 kb on + strand, within Psest_0664 at 723.602 kb on - strand, within Psest_0664 at 723.702 kb on + strand, within Psest_0664 at 723.703 kb on - strand, within Psest_0664 at 723.703 kb on - strand, within Psest_0664 at 723.703 kb on - strand, within Psest_0664 at 723.703 kb on - strand, within Psest_0664 at 723.837 kb on + strand, within Psest_0664 at 723.838 kb on - strand, within Psest_0664 at 723.838 kb on - strand, within Psest_0664 at 723.870 kb on + strand, within Psest_0664 at 723.870 kb on + strand, within Psest_0664 at 723.870 kb on + strand, within Psest_0664 at 723.871 kb on - strand, within Psest_0664 at 723.871 kb on - strand, within Psest_0664 at 723.871 kb on - strand, within Psest_0664 at 723.947 kb on - strand, within Psest_0664
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 720,712 + Psest_0662 0.33 +0.1 720,760 + Psest_0662 0.41 -0.9 720,760 + Psest_0662 0.41 -0.8 720,761 - Psest_0662 0.41 -0.5 721,091 + -3.2 721,255 + +1.0 721,256 - -2.4 721,343 + -0.9 721,619 + Psest_0663 0.22 -2.3 721,619 + Psest_0663 0.22 +0.1 721,620 - Psest_0663 0.22 +0.5 721,620 - Psest_0663 0.22 +0.7 721,620 - Psest_0663 0.22 +0.4 721,620 - Psest_0663 0.22 -0.3 721,845 + Psest_0663 0.35 +2.4 722,176 + Psest_0663 0.53 -1.0 722,176 + Psest_0663 0.53 -0.8 722,176 + Psest_0663 0.53 -0.2 722,176 + Psest_0663 0.53 -0.6 722,177 - Psest_0663 0.53 -1.7 722,177 - Psest_0663 0.53 +0.0 722,177 - Psest_0663 0.53 -0.5 722,401 - Psest_0663 0.66 -2.1 722,567 + Psest_0663 0.75 -0.3 722,626 - Psest_0663 0.78 -0.6 722,666 + Psest_0663 0.80 -1.7 722,761 + Psest_0663 0.86 -1.6 722,872 + -0.1 722,905 + -1.1 722,906 - -1.9 723,024 + -1.3 723,197 - -1.1 723,237 - -1.5 723,427 - Psest_0664 0.18 -0.8 723,601 + Psest_0664 0.29 -0.9 723,601 + Psest_0664 0.29 -1.2 723,602 - Psest_0664 0.30 -2.1 723,702 + Psest_0664 0.36 -2.1 723,703 - Psest_0664 0.36 +1.4 723,703 - Psest_0664 0.36 +0.3 723,703 - Psest_0664 0.36 +1.4 723,703 - Psest_0664 0.36 -0.5 723,837 + Psest_0664 0.45 -0.8 723,838 - Psest_0664 0.45 -0.6 723,838 - Psest_0664 0.45 +0.0 723,870 + Psest_0664 0.47 -1.5 723,870 + Psest_0664 0.47 -1.3 723,870 + Psest_0664 0.47 +0.5 723,871 - Psest_0664 0.48 -0.9 723,871 - Psest_0664 0.48 -2.0 723,871 - Psest_0664 0.48 -1.0 723,947 - Psest_0664 0.53 -1.3
Or see this region's nucleotide sequence