Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0449 and Psest_0450 are separated by 50 nucleotides Psest_0450 and Psest_0451 are separated by 78 nucleotides
Psest_0449: Psest_0449 - Glutamine synthetase adenylyltransferase, at 493,176 to 496,121
_0449
Psest_0450: Psest_0450 - Branched-chain amino acid aminotransferase (EC 2.6.1.42) <small>(from data)</small>, at 496,172 to 497,095
_0450
Psest_0451: Psest_0451 - lipopolysaccharide heptosyltransferase II, at 497,174 to 498,208
_0451
Position (kb) 496
497
498 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 495.177 kb on + strand, within Psest_0449 at 495.492 kb on + strand, within Psest_0449 at 495.492 kb on + strand, within Psest_0449 at 495.492 kb on + strand, within Psest_0449 at 495.492 kb on + strand, within Psest_0449 at 495.492 kb on + strand, within Psest_0449 at 495.493 kb on - strand, within Psest_0449 at 495.493 kb on - strand, within Psest_0449 at 495.854 kb on + strand at 495.854 kb on + strand at 495.864 kb on + strand at 495.909 kb on + strand at 495.910 kb on - strand at 495.911 kb on + strand at 495.911 kb on + strand at 495.993 kb on + strand at 496.094 kb on + strand at 496.094 kb on + strand at 496.094 kb on + strand at 496.094 kb on + strand at 496.268 kb on + strand, within Psest_0450 at 496.460 kb on + strand, within Psest_0450 at 496.460 kb on + strand, within Psest_0450 at 496.481 kb on + strand, within Psest_0450 at 496.481 kb on + strand, within Psest_0450 at 496.559 kb on + strand, within Psest_0450 at 496.559 kb on + strand, within Psest_0450 at 496.664 kb on + strand, within Psest_0450 at 496.664 kb on + strand, within Psest_0450 at 496.664 kb on + strand, within Psest_0450 at 496.664 kb on + strand, within Psest_0450 at 496.664 kb on + strand, within Psest_0450 at 496.664 kb on + strand, within Psest_0450 at 496.742 kb on + strand, within Psest_0450 at 496.796 kb on + strand, within Psest_0450 at 496.796 kb on + strand, within Psest_0450 at 496.796 kb on + strand, within Psest_0450 at 496.796 kb on + strand, within Psest_0450 at 496.796 kb on + strand, within Psest_0450 at 496.796 kb on + strand, within Psest_0450 at 496.878 kb on + strand, within Psest_0450 at 496.878 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 496.910 kb on + strand, within Psest_0450 at 497.033 kb on + strand at 497.033 kb on + strand at 497.033 kb on + strand at 497.093 kb on + strand at 497.093 kb on + strand at 497.093 kb on + strand at 497.093 kb on + strand at 497.093 kb on + strand at 497.159 kb on + strand at 497.166 kb on + strand at 497.166 kb on + strand at 497.166 kb on + strand at 497.173 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 495,177 + Psest_0449 0.68 -0.5 495,492 + Psest_0449 0.79 +0.6 495,492 + Psest_0449 0.79 -0.5 495,492 + Psest_0449 0.79 +0.9 495,492 + Psest_0449 0.79 +0.2 495,492 + Psest_0449 0.79 -0.4 495,493 - Psest_0449 0.79 -2.5 495,493 - Psest_0449 0.79 -1.3 495,854 + +0.7 495,854 + +3.2 495,864 + +0.4 495,909 + +1.0 495,910 - -0.3 495,911 + +1.0 495,911 + +1.2 495,993 + +0.8 496,094 + +1.1 496,094 + +1.2 496,094 + +1.6 496,094 + -0.5 496,268 + Psest_0450 0.10 -2.8 496,460 + Psest_0450 0.31 -2.0 496,460 + Psest_0450 0.31 -2.5 496,481 + Psest_0450 0.33 -2.7 496,481 + Psest_0450 0.33 -1.7 496,559 + Psest_0450 0.42 -2.2 496,559 + Psest_0450 0.42 -2.6 496,664 + Psest_0450 0.53 +0.7 496,664 + Psest_0450 0.53 -0.3 496,664 + Psest_0450 0.53 -1.7 496,664 + Psest_0450 0.53 -1.0 496,664 + Psest_0450 0.53 -0.7 496,664 + Psest_0450 0.53 -2.6 496,742 + Psest_0450 0.62 -2.3 496,796 + Psest_0450 0.68 -2.7 496,796 + Psest_0450 0.68 -3.1 496,796 + Psest_0450 0.68 -3.1 496,796 + Psest_0450 0.68 -2.3 496,796 + Psest_0450 0.68 -2.0 496,796 + Psest_0450 0.68 -0.9 496,878 + Psest_0450 0.76 -1.5 496,878 + Psest_0450 0.76 -2.9 496,910 + Psest_0450 0.80 -2.4 496,910 + Psest_0450 0.80 +0.6 496,910 + Psest_0450 0.80 -3.1 496,910 + Psest_0450 0.80 -3.2 496,910 + Psest_0450 0.80 -1.6 496,910 + Psest_0450 0.80 -1.3 496,910 + Psest_0450 0.80 -2.8 496,910 + Psest_0450 0.80 -1.6 496,910 + Psest_0450 0.80 -4.4 497,033 + -2.5 497,033 + -2.0 497,033 + -2.2 497,093 + +0.5 497,093 + -0.0 497,093 + +0.4 497,093 + +0.5 497,093 + -3.1 497,159 + -0.9 497,166 + -0.5 497,166 + +0.3 497,166 + -0.2 497,173 + -0.4
Or see this region's nucleotide sequence