Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4132 and Psest_4133 are separated by 315 nucleotides Psest_4133 and Psest_4134 are separated by 2 nucleotides Psest_4134 and Psest_4135 are separated by 88 nucleotides
Psest_4132: Psest_4132 - alpha-ketoglutarate DNA-binding response regulator (mifR) (from data) , at 4,312,452 to 4,313,795
_4132
Psest_4133: Psest_4133 - RNA polymerase sigma factor, sigma-70 family, at 4,314,111 to 4,315,352
_4133
Psest_4134: Psest_4134 - Uncharacterized protein conserved in bacteria, at 4,315,355 to 4,315,711
_4134
Psest_4135: Psest_4135 - Ketosteroid isomerase homolog, at 4,315,800 to 4,316,258
_4135
Position (kb)
4314
4315
4316 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4313.255 kb on - strand, within Psest_4132 at 4313.384 kb on + strand, within Psest_4132 at 4313.384 kb on + strand, within Psest_4132 at 4313.385 kb on - strand, within Psest_4132 at 4313.385 kb on - strand, within Psest_4132 at 4313.766 kb on + strand at 4313.766 kb on + strand at 4313.766 kb on + strand at 4313.766 kb on + strand at 4313.766 kb on + strand at 4313.767 kb on - strand at 4313.767 kb on - strand at 4313.767 kb on - strand at 4313.767 kb on - strand at 4313.767 kb on - strand at 4313.789 kb on + strand at 4314.021 kb on + strand at 4314.022 kb on - strand at 4314.053 kb on - strand at 4314.053 kb on - strand at 4314.053 kb on - strand at 4314.053 kb on - strand at 4314.213 kb on - strand at 4314.213 kb on - strand at 4314.213 kb on - strand at 4314.213 kb on - strand at 4314.262 kb on + strand, within Psest_4133 at 4314.397 kb on + strand, within Psest_4133 at 4314.398 kb on - strand, within Psest_4133 at 4314.577 kb on - strand, within Psest_4133 at 4314.757 kb on + strand, within Psest_4133 at 4314.758 kb on - strand, within Psest_4133 at 4314.758 kb on - strand, within Psest_4133 at 4314.758 kb on - strand, within Psest_4133 at 4314.758 kb on - strand, within Psest_4133 at 4314.778 kb on + strand, within Psest_4133 at 4314.778 kb on + strand, within Psest_4133 at 4314.778 kb on + strand, within Psest_4133 at 4314.779 kb on - strand, within Psest_4133 at 4314.779 kb on - strand, within Psest_4133 at 4314.779 kb on - strand, within Psest_4133 at 4314.779 kb on - strand, within Psest_4133 at 4314.882 kb on - strand, within Psest_4133 at 4315.183 kb on - strand, within Psest_4133 at 4315.306 kb on + strand at 4315.467 kb on + strand, within Psest_4134 at 4315.467 kb on + strand, within Psest_4134 at 4315.467 kb on + strand, within Psest_4134 at 4315.468 kb on - strand, within Psest_4134 at 4315.468 kb on - strand, within Psest_4134 at 4315.687 kb on - strand at 4315.687 kb on - strand at 4315.689 kb on + strand at 4315.689 kb on + strand at 4315.689 kb on + strand at 4315.689 kb on + strand at 4315.689 kb on + strand at 4315.689 kb on + strand at 4315.690 kb on - strand at 4315.690 kb on - strand at 4315.690 kb on - strand at 4315.690 kb on - strand at 4315.690 kb on - strand at 4315.690 kb on - strand at 4315.704 kb on + strand at 4315.704 kb on + strand at 4315.704 kb on + strand at 4315.705 kb on - strand at 4315.705 kb on - strand at 4315.705 kb on - strand at 4315.705 kb on - strand at 4315.746 kb on + strand at 4315.747 kb on - strand at 4315.856 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.142 kb on + strand, within Psest_4135 at 4316.143 kb on - strand, within Psest_4135 at 4316.143 kb on - strand, within Psest_4135 at 4316.143 kb on - strand, within Psest_4135 at 4316.143 kb on - strand, within Psest_4135 at 4316.299 kb on + strand at 4316.299 kb on + strand at 4316.299 kb on + strand at 4316.299 kb on + strand at 4316.299 kb on + strand at 4316.300 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,313,255 - Psest_4132 0.60 +0.0 4,313,384 + Psest_4132 0.69 -2.6 4,313,384 + Psest_4132 0.69 -0.9 4,313,385 - Psest_4132 0.69 -0.2 4,313,385 - Psest_4132 0.69 +1.5 4,313,766 + -1.0 4,313,766 + -2.5 4,313,766 + -0.4 4,313,766 + +0.5 4,313,766 + -1.9 4,313,767 - +0.3 4,313,767 - -0.3 4,313,767 - +0.2 4,313,767 - +1.8 4,313,767 - -1.0 4,313,789 + -1.7 4,314,021 + -2.6 4,314,022 - -1.1 4,314,053 - +1.9 4,314,053 - -2.2 4,314,053 - -1.1 4,314,053 - -1.1 4,314,213 - +0.3 4,314,213 - -1.7 4,314,213 - -2.6 4,314,213 - -0.7 4,314,262 + Psest_4133 0.12 +1.1 4,314,397 + Psest_4133 0.23 -2.2 4,314,398 - Psest_4133 0.23 -1.9 4,314,577 - Psest_4133 0.38 -1.0 4,314,757 + Psest_4133 0.52 -1.9 4,314,758 - Psest_4133 0.52 -2.5 4,314,758 - Psest_4133 0.52 -1.0 4,314,758 - Psest_4133 0.52 -0.1 4,314,758 - Psest_4133 0.52 -2.7 4,314,778 + Psest_4133 0.54 -0.5 4,314,778 + Psest_4133 0.54 -0.1 4,314,778 + Psest_4133 0.54 -0.3 4,314,779 - Psest_4133 0.54 -1.3 4,314,779 - Psest_4133 0.54 -1.3 4,314,779 - Psest_4133 0.54 -0.9 4,314,779 - Psest_4133 0.54 -2.2 4,314,882 - Psest_4133 0.62 -0.6 4,315,183 - Psest_4133 0.86 -0.4 4,315,306 + -1.7 4,315,467 + Psest_4134 0.31 -0.7 4,315,467 + Psest_4134 0.31 -0.1 4,315,467 + Psest_4134 0.31 -0.2 4,315,468 - Psest_4134 0.32 +1.7 4,315,468 - Psest_4134 0.32 -0.1 4,315,687 - -0.4 4,315,687 - -0.4 4,315,689 + -2.1 4,315,689 + -3.0 4,315,689 + -1.0 4,315,689 + -1.1 4,315,689 + -2.4 4,315,689 + -0.5 4,315,690 - -3.0 4,315,690 - +1.3 4,315,690 - -1.9 4,315,690 - -2.8 4,315,690 - -1.0 4,315,690 - -2.2 4,315,704 + -2.4 4,315,704 + -1.4 4,315,704 + -0.8 4,315,705 - -0.1 4,315,705 - -0.5 4,315,705 - -0.6 4,315,705 - -2.4 4,315,746 + -1.1 4,315,747 - -0.8 4,315,856 + Psest_4135 0.12 -0.9 4,316,142 + Psest_4135 0.75 +0.2 4,316,142 + Psest_4135 0.75 +0.0 4,316,142 + Psest_4135 0.75 -0.1 4,316,142 + Psest_4135 0.75 -0.8 4,316,142 + Psest_4135 0.75 -0.2 4,316,142 + Psest_4135 0.75 -1.1 4,316,142 + Psest_4135 0.75 -0.7 4,316,142 + Psest_4135 0.75 +0.9 4,316,142 + Psest_4135 0.75 -1.8 4,316,143 - Psest_4135 0.75 -0.9 4,316,143 - Psest_4135 0.75 -1.9 4,316,143 - Psest_4135 0.75 +0.8 4,316,143 - Psest_4135 0.75 -2.2 4,316,299 + -1.9 4,316,299 + -0.4 4,316,299 + +0.5 4,316,299 + +1.2 4,316,299 + +0.5 4,316,300 - -1.5
Or see this region's nucleotide sequence