Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_4051 and Psest_4052 are separated by 20 nucleotides Psest_4052 and Psest_4053 overlap by 4 nucleotides Psest_4053 and Psest_4054 are separated by 115 nucleotides
Psest_4051: Psest_4051 - molybdenum-pterin binding domain, at 4,224,947 to 4,225,162
_4051
Psest_4052: Psest_4052 - ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component, at 4,225,183 to 4,225,983
_4052
Psest_4053: Psest_4053 - ABC-type nitrate/sulfonate/bicarbonate transport system, permease component, at 4,225,980 to 4,226,764
_4053
Psest_4054: Psest_4054 - alkanesulfonate monooxygenase, FMNH(2)-dependent, at 4,226,880 to 4,228,028
_4054
Position (kb)
4225
4226
4227 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4225.073 kb on + strand, within Psest_4051 at 4225.074 kb on - strand, within Psest_4051 at 4225.074 kb on - strand, within Psest_4051 at 4225.074 kb on - strand, within Psest_4051 at 4225.074 kb on - strand, within Psest_4051 at 4225.184 kb on + strand at 4225.270 kb on + strand, within Psest_4052 at 4225.270 kb on + strand, within Psest_4052 at 4225.372 kb on + strand, within Psest_4052 at 4225.474 kb on + strand, within Psest_4052 at 4225.475 kb on - strand, within Psest_4052 at 4225.475 kb on - strand, within Psest_4052 at 4225.475 kb on - strand, within Psest_4052 at 4225.475 kb on - strand, within Psest_4052 at 4225.538 kb on - strand, within Psest_4052 at 4225.538 kb on - strand, within Psest_4052 at 4225.904 kb on - strand at 4225.941 kb on + strand at 4225.942 kb on - strand at 4225.942 kb on - strand at 4226.005 kb on + strand at 4226.006 kb on - strand at 4226.342 kb on - strand, within Psest_4053 at 4226.342 kb on - strand, within Psest_4053 at 4226.342 kb on - strand, within Psest_4053 at 4226.353 kb on + strand, within Psest_4053 at 4226.353 kb on + strand, within Psest_4053 at 4226.353 kb on + strand, within Psest_4053 at 4226.353 kb on + strand, within Psest_4053 at 4226.353 kb on + strand, within Psest_4053 at 4226.354 kb on - strand, within Psest_4053 at 4226.354 kb on - strand, within Psest_4053 at 4226.354 kb on - strand, within Psest_4053 at 4226.354 kb on - strand, within Psest_4053 at 4226.354 kb on - strand, within Psest_4053 at 4226.354 kb on - strand, within Psest_4053 at 4226.781 kb on - strand at 4226.800 kb on + strand at 4226.801 kb on - strand at 4226.878 kb on - strand at 4226.945 kb on - strand at 4227.073 kb on + strand, within Psest_4054 at 4227.074 kb on - strand, within Psest_4054 at 4227.448 kb on + strand, within Psest_4054 at 4227.448 kb on + strand, within Psest_4054 at 4227.448 kb on + strand, within Psest_4054 at 4227.449 kb on - strand, within Psest_4054 at 4227.449 kb on - strand, within Psest_4054 at 4227.505 kb on + strand, within Psest_4054 at 4227.581 kb on - strand, within Psest_4054 at 4227.581 kb on - strand, within Psest_4054
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,225,073 + Psest_4051 0.58 -2.2 4,225,074 - Psest_4051 0.59 +1.1 4,225,074 - Psest_4051 0.59 +0.6 4,225,074 - Psest_4051 0.59 -2.7 4,225,074 - Psest_4051 0.59 -1.4 4,225,184 + -0.7 4,225,270 + Psest_4052 0.11 -1.5 4,225,270 + Psest_4052 0.11 -0.7 4,225,372 + Psest_4052 0.24 -0.5 4,225,474 + Psest_4052 0.36 -1.2 4,225,475 - Psest_4052 0.36 -1.0 4,225,475 - Psest_4052 0.36 -0.2 4,225,475 - Psest_4052 0.36 +0.6 4,225,475 - Psest_4052 0.36 -0.9 4,225,538 - Psest_4052 0.44 +0.3 4,225,538 - Psest_4052 0.44 +0.6 4,225,904 - +0.3 4,225,941 + -1.2 4,225,942 - -0.9 4,225,942 - +0.8 4,226,005 + +0.9 4,226,006 - +0.6 4,226,342 - Psest_4053 0.46 -1.5 4,226,342 - Psest_4053 0.46 +0.1 4,226,342 - Psest_4053 0.46 -0.9 4,226,353 + Psest_4053 0.48 -1.5 4,226,353 + Psest_4053 0.48 -0.7 4,226,353 + Psest_4053 0.48 -0.2 4,226,353 + Psest_4053 0.48 -1.4 4,226,353 + Psest_4053 0.48 -1.5 4,226,354 - Psest_4053 0.48 -0.9 4,226,354 - Psest_4053 0.48 -1.2 4,226,354 - Psest_4053 0.48 -1.7 4,226,354 - Psest_4053 0.48 -0.9 4,226,354 - Psest_4053 0.48 -1.3 4,226,354 - Psest_4053 0.48 -2.7 4,226,781 - -0.2 4,226,800 + +0.5 4,226,801 - -0.2 4,226,878 - +0.3 4,226,945 - -0.7 4,227,073 + Psest_4054 0.17 -2.6 4,227,074 - Psest_4054 0.17 -0.5 4,227,448 + Psest_4054 0.49 +1.9 4,227,448 + Psest_4054 0.49 +0.3 4,227,448 + Psest_4054 0.49 -0.1 4,227,449 - Psest_4054 0.50 -0.7 4,227,449 - Psest_4054 0.50 -0.2 4,227,505 + Psest_4054 0.54 +1.1 4,227,581 - Psest_4054 0.61 -2.2 4,227,581 - Psest_4054 0.61 -0.7
Or see this region's nucleotide sequence