Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3836 and Psest_3837 are separated by 217 nucleotides Psest_3837 and Psest_3838 are separated by 20 nucleotides Psest_3838 and Psest_3839 are separated by 73 nucleotides
Psest_3836: Psest_3836 - L-lactate permease, at 4,005,379 to 4,007,082
_3836
Psest_3837: Psest_3837 - malate synthase G, at 4,007,300 to 4,009,480
_3837
Psest_3838: Psest_3838 - Uncharacterized protein, possibly involved in utilization of glycolate and propanediol, at 4,009,501 to 4,009,905
_3838
Psest_3839: Psest_3839 - D-lactate/glycolate dehydrogenase, iron-sulfur subunit GlcF (EC 1.1.99.6; EC 1.1.99.14) (from data) , at 4,009,979 to 4,011,196
_3839
Position (kb)
4007
4008
4009
4010 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4006.335 kb on - strand, within Psest_3836 at 4006.521 kb on + strand, within Psest_3836 at 4006.994 kb on + strand at 4006.995 kb on - strand at 4006.995 kb on - strand at 4006.995 kb on - strand at 4007.068 kb on + strand at 4007.068 kb on + strand at 4007.068 kb on + strand at 4007.068 kb on + strand at 4007.069 kb on - strand at 4007.069 kb on - strand at 4007.120 kb on + strand at 4007.120 kb on + strand at 4007.120 kb on + strand at 4007.121 kb on - strand at 4007.121 kb on - strand at 4007.121 kb on - strand at 4007.121 kb on - strand at 4007.121 kb on - strand at 4007.121 kb on - strand at 4007.121 kb on - strand at 4007.121 kb on - strand at 4007.121 kb on - strand at 4007.121 kb on - strand at 4007.121 kb on - strand at 4007.121 kb on - strand at 4007.121 kb on - strand at 4007.319 kb on + strand at 4007.320 kb on - strand at 4007.424 kb on + strand at 4007.424 kb on + strand at 4007.424 kb on + strand at 4007.425 kb on - strand at 4007.425 kb on - strand at 4007.566 kb on + strand, within Psest_3837 at 4007.566 kb on + strand, within Psest_3837 at 4007.567 kb on - strand, within Psest_3837 at 4007.567 kb on - strand, within Psest_3837 at 4007.567 kb on - strand, within Psest_3837 at 4007.567 kb on - strand, within Psest_3837 at 4007.658 kb on + strand, within Psest_3837 at 4007.659 kb on - strand, within Psest_3837 at 4008.315 kb on + strand, within Psest_3837 at 4008.315 kb on + strand, within Psest_3837 at 4008.315 kb on + strand, within Psest_3837 at 4008.315 kb on + strand, within Psest_3837 at 4008.315 kb on + strand, within Psest_3837 at 4008.316 kb on - strand, within Psest_3837 at 4008.316 kb on - strand, within Psest_3837 at 4008.316 kb on - strand, within Psest_3837 at 4008.519 kb on + strand, within Psest_3837 at 4008.520 kb on - strand, within Psest_3837 at 4009.065 kb on + strand, within Psest_3837 at 4009.065 kb on + strand, within Psest_3837 at 4009.105 kb on - strand, within Psest_3837 at 4009.739 kb on + strand, within Psest_3838 at 4009.740 kb on - strand, within Psest_3838 at 4009.831 kb on - strand, within Psest_3838 at 4009.925 kb on + strand at 4009.926 kb on - strand at 4009.926 kb on - strand at 4009.926 kb on - strand at 4009.926 kb on - strand at 4010.157 kb on + strand, within Psest_3839 at 4010.158 kb on - strand, within Psest_3839
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 4,006,335 - Psest_3836 0.56 +0.4 4,006,521 + Psest_3836 0.67 -0.4 4,006,994 + -0.7 4,006,995 - -0.1 4,006,995 - -1.9 4,006,995 - +1.1 4,007,068 + +0.9 4,007,068 + +2.6 4,007,068 + -1.0 4,007,068 + -1.3 4,007,069 - +2.5 4,007,069 - +0.4 4,007,120 + -0.6 4,007,120 + +0.0 4,007,120 + +0.3 4,007,121 - +0.6 4,007,121 - -1.1 4,007,121 - +0.3 4,007,121 - +1.3 4,007,121 - +1.2 4,007,121 - -0.4 4,007,121 - -1.6 4,007,121 - -0.1 4,007,121 - -0.1 4,007,121 - +2.1 4,007,121 - -0.4 4,007,121 - -0.1 4,007,121 - +0.5 4,007,319 + +3.5 4,007,320 - -0.0 4,007,424 + -0.1 4,007,424 + +0.0 4,007,424 + +1.9 4,007,425 - -1.1 4,007,425 - +0.5 4,007,566 + Psest_3837 0.12 -0.4 4,007,566 + Psest_3837 0.12 +0.7 4,007,567 - Psest_3837 0.12 +0.7 4,007,567 - Psest_3837 0.12 -0.6 4,007,567 - Psest_3837 0.12 -0.2 4,007,567 - Psest_3837 0.12 -0.1 4,007,658 + Psest_3837 0.16 -1.6 4,007,659 - Psest_3837 0.16 -0.1 4,008,315 + Psest_3837 0.47 +0.9 4,008,315 + Psest_3837 0.47 +0.9 4,008,315 + Psest_3837 0.47 +0.2 4,008,315 + Psest_3837 0.47 -1.0 4,008,315 + Psest_3837 0.47 -1.2 4,008,316 - Psest_3837 0.47 +0.3 4,008,316 - Psest_3837 0.47 +1.1 4,008,316 - Psest_3837 0.47 +0.8 4,008,519 + Psest_3837 0.56 -2.2 4,008,520 - Psest_3837 0.56 -1.7 4,009,065 + Psest_3837 0.81 -1.4 4,009,065 + Psest_3837 0.81 +0.5 4,009,105 - Psest_3837 0.83 -0.0 4,009,739 + Psest_3838 0.59 -1.6 4,009,740 - Psest_3838 0.59 +0.3 4,009,831 - Psest_3838 0.81 +0.3 4,009,925 + -0.4 4,009,926 - +0.3 4,009,926 - +2.8 4,009,926 - -0.7 4,009,926 - +0.3 4,010,157 + Psest_3839 0.15 +1.2 4,010,158 - Psest_3839 0.15 +0.3
Or see this region's nucleotide sequence