Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_0316 and Psest_0317 are separated by 9 nucleotides Psest_0317 and Psest_0318 are separated by 60 nucleotides Psest_0318 and Psest_0319 are separated by 93 nucleotides
Psest_0316: Psest_0316 - Cytochrome c556, at 339,459 to 339,905
_0316
Psest_0317: Psest_0317 - Protein of unknown function (DUF1090)., at 339,915 to 340,295
_0317
Psest_0318: Psest_0318 - NAD-dependent DNA ligase (contains BRCT domain type II), at 340,356 to 342,020
_0318
Psest_0319: Psest_0319 - Predicted ferric reductase, at 342,114 to 343,445
_0319
Position (kb) 340
341
342
343 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 339.425 kb on - strand at 339.503 kb on - strand at 339.503 kb on - strand at 339.561 kb on + strand, within Psest_0316 at 339.562 kb on - strand, within Psest_0316 at 339.562 kb on - strand, within Psest_0316 at 339.562 kb on - strand, within Psest_0316 at 339.916 kb on + strand at 339.917 kb on - strand at 340.032 kb on + strand, within Psest_0317 at 340.055 kb on - strand, within Psest_0317 at 340.308 kb on + strand at 340.308 kb on + strand at 340.308 kb on + strand at 340.308 kb on + strand at 340.309 kb on - strand at 340.309 kb on - strand at 340.309 kb on - strand at 340.327 kb on - strand at 340.371 kb on - strand at 340.371 kb on - strand at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.910 kb on + strand, within Psest_0318 at 340.911 kb on - strand, within Psest_0318 at 340.911 kb on - strand, within Psest_0318 at 340.911 kb on - strand, within Psest_0318 at 340.911 kb on - strand, within Psest_0318 at 340.911 kb on - strand, within Psest_0318 at 340.911 kb on - strand, within Psest_0318 at 340.911 kb on - strand, within Psest_0318 at 340.911 kb on - strand, within Psest_0318 at 340.974 kb on + strand, within Psest_0318 at 341.102 kb on + strand, within Psest_0318 at 341.102 kb on + strand, within Psest_0318 at 341.102 kb on + strand, within Psest_0318 at 341.103 kb on - strand, within Psest_0318 at 341.114 kb on - strand, within Psest_0318 at 341.114 kb on - strand, within Psest_0318 at 341.114 kb on - strand, within Psest_0318 at 341.229 kb on + strand, within Psest_0318 at 341.229 kb on + strand, within Psest_0318 at 341.229 kb on + strand, within Psest_0318 at 341.229 kb on + strand, within Psest_0318 at 341.598 kb on - strand, within Psest_0318 at 341.598 kb on - strand, within Psest_0318 at 341.884 kb on + strand at 341.885 kb on - strand at 341.885 kb on - strand at 342.122 kb on + strand at 342.122 kb on + strand at 342.122 kb on + strand at 342.123 kb on - strand at 342.297 kb on + strand, within Psest_0319 at 342.376 kb on + strand, within Psest_0319 at 342.377 kb on - strand, within Psest_0319 at 342.424 kb on - strand, within Psest_0319 at 342.569 kb on - strand, within Psest_0319 at 342.720 kb on - strand, within Psest_0319 at 342.897 kb on + strand, within Psest_0319 at 342.898 kb on - strand, within Psest_0319 at 342.898 kb on - strand, within Psest_0319 at 342.898 kb on - strand, within Psest_0319 at 342.898 kb on - strand, within Psest_0319 at 342.925 kb on + strand, within Psest_0319 at 342.925 kb on + strand, within Psest_0319 at 342.926 kb on - strand, within Psest_0319 at 342.926 kb on - strand, within Psest_0319 at 342.926 kb on - strand, within Psest_0319 at 343.016 kb on - strand, within Psest_0319
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 339,425 - -0.1 339,503 - +0.0 339,503 - +0.7 339,561 + Psest_0316 0.23 -1.2 339,562 - Psest_0316 0.23 -1.1 339,562 - Psest_0316 0.23 +2.1 339,562 - Psest_0316 0.23 -0.7 339,916 + -1.7 339,917 - -1.3 340,032 + Psest_0317 0.31 +0.6 340,055 - Psest_0317 0.37 -0.7 340,308 + -1.1 340,308 + -0.4 340,308 + -1.2 340,308 + -1.7 340,309 - -1.2 340,309 - -1.0 340,309 - -0.7 340,327 - -1.0 340,371 - -1.3 340,371 - -0.2 340,910 + Psest_0318 0.33 -0.7 340,910 + Psest_0318 0.33 +0.2 340,910 + Psest_0318 0.33 -2.3 340,910 + Psest_0318 0.33 -0.2 340,910 + Psest_0318 0.33 -2.2 340,910 + Psest_0318 0.33 +0.4 340,910 + Psest_0318 0.33 -3.3 340,910 + Psest_0318 0.33 +1.4 340,910 + Psest_0318 0.33 +0.2 340,910 + Psest_0318 0.33 -0.8 340,910 + Psest_0318 0.33 -1.5 340,910 + Psest_0318 0.33 -2.1 340,910 + Psest_0318 0.33 -1.8 340,910 + Psest_0318 0.33 -1.2 340,910 + Psest_0318 0.33 +0.0 340,911 - Psest_0318 0.33 +1.0 340,911 - Psest_0318 0.33 +0.4 340,911 - Psest_0318 0.33 -1.8 340,911 - Psest_0318 0.33 -1.8 340,911 - Psest_0318 0.33 +1.6 340,911 - Psest_0318 0.33 +0.2 340,911 - Psest_0318 0.33 -1.8 340,911 - Psest_0318 0.33 +0.8 340,974 + Psest_0318 0.37 -1.5 341,102 + Psest_0318 0.45 -1.0 341,102 + Psest_0318 0.45 -2.0 341,102 + Psest_0318 0.45 -1.0 341,103 - Psest_0318 0.45 -1.5 341,114 - Psest_0318 0.46 +0.8 341,114 - Psest_0318 0.46 -1.2 341,114 - Psest_0318 0.46 -1.6 341,229 + Psest_0318 0.52 -0.5 341,229 + Psest_0318 0.52 -1.5 341,229 + Psest_0318 0.52 -1.5 341,229 + Psest_0318 0.52 -1.3 341,598 - Psest_0318 0.75 -0.5 341,598 - Psest_0318 0.75 +1.2 341,884 + +0.1 341,885 - -2.0 341,885 - -2.4 342,122 + -0.2 342,122 + +0.9 342,122 + +0.5 342,123 - +0.6 342,297 + Psest_0319 0.14 +0.4 342,376 + Psest_0319 0.20 +1.1 342,377 - Psest_0319 0.20 +0.0 342,424 - Psest_0319 0.23 +0.5 342,569 - Psest_0319 0.34 +0.1 342,720 - Psest_0319 0.45 -2.9 342,897 + Psest_0319 0.59 -0.6 342,898 - Psest_0319 0.59 +0.7 342,898 - Psest_0319 0.59 -2.0 342,898 - Psest_0319 0.59 +0.2 342,898 - Psest_0319 0.59 -0.2 342,925 + Psest_0319 0.61 -1.6 342,925 + Psest_0319 0.61 +0.8 342,926 - Psest_0319 0.61 -0.8 342,926 - Psest_0319 0.61 -0.5 342,926 - Psest_0319 0.61 -1.4 343,016 - Psest_0319 0.68 -1.6
Or see this region's nucleotide sequence