Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3185 and Psest_3186 overlap by 4 nucleotides Psest_3186 and Psest_3187 are separated by 5 nucleotides Psest_3187 and Psest_3188 are separated by 15 nucleotides Psest_3188 and Psest_3189 are separated by 25 nucleotides
Psest_3185: Psest_3185 - MoxR-like ATPases, at 3,357,717 to 3,358,730
_3185
Psest_3186: Psest_3186 - Uncharacterized conserved protein (some members contain a von Willebrand factor type A (vWA) domain), at 3,358,727 to 3,360,055
_3186
Psest_3187: Psest_3187 - hypothetical protein, at 3,360,061 to 3,360,519
_3187
Psest_3188: Psest_3188 - hypothetical protein, at 3,360,535 to 3,360,957
_3188
Psest_3189: Psest_3189 - hypothetical protein, at 3,360,983 to 3,361,360
_3189
Position (kb)
3358
3359
3360
3361 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3357.832 kb on + strand, within Psest_3185 at 3358.027 kb on - strand, within Psest_3185 at 3358.142 kb on - strand, within Psest_3185 at 3358.461 kb on + strand, within Psest_3185 at 3358.907 kb on - strand, within Psest_3186 at 3358.907 kb on - strand, within Psest_3186 at 3358.966 kb on - strand, within Psest_3186 at 3359.064 kb on + strand, within Psest_3186 at 3359.100 kb on - strand, within Psest_3186 at 3359.100 kb on - strand, within Psest_3186 at 3359.152 kb on - strand, within Psest_3186 at 3359.152 kb on - strand, within Psest_3186 at 3359.152 kb on - strand, within Psest_3186 at 3359.152 kb on - strand, within Psest_3186 at 3359.420 kb on + strand, within Psest_3186 at 3359.473 kb on + strand, within Psest_3186 at 3359.474 kb on - strand, within Psest_3186 at 3359.474 kb on - strand, within Psest_3186 at 3359.474 kb on - strand, within Psest_3186 at 3359.658 kb on - strand, within Psest_3186 at 3359.658 kb on - strand, within Psest_3186 at 3359.658 kb on - strand, within Psest_3186 at 3359.713 kb on - strand, within Psest_3186 at 3359.922 kb on - strand, within Psest_3186 at 3359.947 kb on + strand at 3360.054 kb on - strand at 3360.158 kb on + strand, within Psest_3187 at 3360.158 kb on + strand, within Psest_3187 at 3360.159 kb on - strand, within Psest_3187 at 3360.159 kb on - strand, within Psest_3187 at 3360.159 kb on - strand, within Psest_3187 at 3360.159 kb on - strand, within Psest_3187 at 3360.159 kb on - strand, within Psest_3187 at 3360.159 kb on - strand, within Psest_3187 at 3360.228 kb on - strand, within Psest_3187 at 3360.240 kb on + strand, within Psest_3187 at 3360.430 kb on + strand, within Psest_3187 at 3360.430 kb on + strand, within Psest_3187 at 3360.430 kb on + strand, within Psest_3187 at 3360.430 kb on + strand, within Psest_3187 at 3360.430 kb on + strand, within Psest_3187 at 3360.431 kb on - strand, within Psest_3187 at 3360.431 kb on - strand, within Psest_3187 at 3360.431 kb on - strand, within Psest_3187 at 3360.440 kb on + strand, within Psest_3187 at 3360.440 kb on + strand, within Psest_3187 at 3360.441 kb on - strand, within Psest_3187 at 3360.586 kb on + strand, within Psest_3188 at 3360.587 kb on - strand, within Psest_3188 at 3360.587 kb on - strand, within Psest_3188 at 3360.603 kb on - strand, within Psest_3188 at 3360.606 kb on - strand, within Psest_3188 at 3360.606 kb on - strand, within Psest_3188 at 3360.900 kb on - strand, within Psest_3188 at 3360.900 kb on - strand, within Psest_3188 at 3360.900 kb on - strand, within Psest_3188 at 3361.027 kb on - strand, within Psest_3189
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,357,832 + Psest_3185 0.11 +0.0 3,358,027 - Psest_3185 0.31 -2.1 3,358,142 - Psest_3185 0.42 +0.0 3,358,461 + Psest_3185 0.73 -2.4 3,358,907 - Psest_3186 0.14 -1.6 3,358,907 - Psest_3186 0.14 -1.0 3,358,966 - Psest_3186 0.18 +0.6 3,359,064 + Psest_3186 0.25 -2.2 3,359,100 - Psest_3186 0.28 +0.1 3,359,100 - Psest_3186 0.28 -1.7 3,359,152 - Psest_3186 0.32 +2.2 3,359,152 - Psest_3186 0.32 -1.0 3,359,152 - Psest_3186 0.32 -2.9 3,359,152 - Psest_3186 0.32 -1.0 3,359,420 + Psest_3186 0.52 -0.6 3,359,473 + Psest_3186 0.56 -0.5 3,359,474 - Psest_3186 0.56 -2.1 3,359,474 - Psest_3186 0.56 -0.3 3,359,474 - Psest_3186 0.56 -0.4 3,359,658 - Psest_3186 0.70 -1.2 3,359,658 - Psest_3186 0.70 +1.3 3,359,658 - Psest_3186 0.70 -2.5 3,359,713 - Psest_3186 0.74 -0.8 3,359,922 - Psest_3186 0.90 -1.5 3,359,947 + +0.6 3,360,054 - -0.4 3,360,158 + Psest_3187 0.21 -2.0 3,360,158 + Psest_3187 0.21 -0.1 3,360,159 - Psest_3187 0.21 +0.0 3,360,159 - Psest_3187 0.21 -2.2 3,360,159 - Psest_3187 0.21 -1.4 3,360,159 - Psest_3187 0.21 +0.0 3,360,159 - Psest_3187 0.21 -2.1 3,360,159 - Psest_3187 0.21 -0.9 3,360,228 - Psest_3187 0.36 -0.4 3,360,240 + Psest_3187 0.39 +1.0 3,360,430 + Psest_3187 0.80 +1.0 3,360,430 + Psest_3187 0.80 +0.0 3,360,430 + Psest_3187 0.80 -1.2 3,360,430 + Psest_3187 0.80 -0.1 3,360,430 + Psest_3187 0.80 +1.2 3,360,431 - Psest_3187 0.81 +0.6 3,360,431 - Psest_3187 0.81 -2.2 3,360,431 - Psest_3187 0.81 -1.0 3,360,440 + Psest_3187 0.83 -1.2 3,360,440 + Psest_3187 0.83 -1.9 3,360,441 - Psest_3187 0.83 -2.2 3,360,586 + Psest_3188 0.12 -1.9 3,360,587 - Psest_3188 0.12 -1.7 3,360,587 - Psest_3188 0.12 -1.2 3,360,603 - Psest_3188 0.16 -0.4 3,360,606 - Psest_3188 0.17 +1.6 3,360,606 - Psest_3188 0.17 +0.9 3,360,900 - Psest_3188 0.86 +0.0 3,360,900 - Psest_3188 0.86 -0.9 3,360,900 - Psest_3188 0.86 -0.2 3,361,027 - Psest_3189 0.12 +0.9
Or see this region's nucleotide sequence