Experiment: m.b. D-Cycloserine 0.032 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HP15_3063 and HP15_3064 are separated by 288 nucleotides HP15_3064 and HP15_3065 are separated by 78 nucleotides HP15_3065 and HP15_3066 overlap by 4 nucleotides HP15_3066 and HP15_3067 overlap by 1 nucleotides
HP15_3063: HP15_3063 - polynucleotide phosphorylase/polyadenylase, at 3,214,408 to 3,216,591
_3063
HP15_3064: HP15_3064 - ribosomal protein S15, bacterial-type, at 3,216,880 to 3,217,149
_3064
HP15_3065: HP15_3065 - tRNA pseudouridine synthase B, at 3,217,228 to 3,218,169
_3065
HP15_3066: HP15_3066 - ribosome-binding factor A, at 3,218,166 to 3,218,588
_3066
HP15_3067: HP15_3067 - translation initiation factor IF-2, at 3,218,588 to 3,221,137
_3067
Position (kb)
3217
3218
3219 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3216.854 kb on - strand at 3217.437 kb on - strand, within HP15_3065 at 3217.439 kb on + strand, within HP15_3065 at 3217.529 kb on + strand, within HP15_3065 at 3217.567 kb on - strand, within HP15_3065 at 3217.574 kb on + strand, within HP15_3065 at 3217.576 kb on + strand, within HP15_3065 at 3217.591 kb on + strand, within HP15_3065 at 3217.599 kb on - strand, within HP15_3065 at 3217.599 kb on - strand, within HP15_3065 at 3217.638 kb on - strand, within HP15_3065 at 3217.795 kb on - strand, within HP15_3065 at 3217.796 kb on + strand, within HP15_3065 at 3217.922 kb on + strand, within HP15_3065 at 3217.937 kb on + strand, within HP15_3065 at 3217.942 kb on + strand, within HP15_3065 at 3217.943 kb on + strand, within HP15_3065 at 3218.463 kb on + strand, within HP15_3066
Per-strain Table
Position Strand Gene LocusTag Fraction m.b. D-Cycloserine 0.032 mg/ml remove 3,216,854 - -2.3 3,217,437 - HP15_3065 0.22 -0.8 3,217,439 + HP15_3065 0.22 -0.3 3,217,529 + HP15_3065 0.32 +0.1 3,217,567 - HP15_3065 0.36 -0.8 3,217,574 + HP15_3065 0.37 +0.4 3,217,576 + HP15_3065 0.37 -0.7 3,217,591 + HP15_3065 0.39 -0.6 3,217,599 - HP15_3065 0.39 -0.5 3,217,599 - HP15_3065 0.39 -1.6 3,217,638 - HP15_3065 0.44 -0.0 3,217,795 - HP15_3065 0.60 -1.3 3,217,796 + HP15_3065 0.60 -0.5 3,217,922 + HP15_3065 0.74 +0.5 3,217,937 + HP15_3065 0.75 +1.7 3,217,942 + HP15_3065 0.76 -1.2 3,217,943 + HP15_3065 0.76 -0.8 3,218,463 + HP15_3066 0.70 -3.6
Or see this region's nucleotide sequence