Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_3159 and Psest_3160 are separated by 125 nucleotides Psest_3160 and Psest_3161 are separated by 9 nucleotides Psest_3161 and Psest_3162 are separated by 4 nucleotides
Psest_3159: Psest_3159 - phosphoribosylformylglycinamidine synthase, single chain form, at 3,328,470 to 3,332,366
_3159
Psest_3160: Psest_3160 - ABC-type polar amino acid transport system, ATPase component, at 3,332,492 to 3,333,259
_3160
Psest_3161: Psest_3161 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 3,333,269 to 3,334,366
_3161
Psest_3162: Psest_3162 - amine acid ABC transporter, permease protein, 3-TM region, His/Glu/Gln/Arg/opine family, at 3,334,371 to 3,335,555
_3162
Position (kb)
3333
3334
3335 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3332.269 kb on + strand at 3332.406 kb on + strand at 3332.406 kb on + strand at 3332.406 kb on + strand at 3332.406 kb on + strand at 3332.406 kb on + strand at 3332.406 kb on + strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.407 kb on - strand at 3332.411 kb on + strand at 3332.411 kb on + strand at 3332.686 kb on + strand, within Psest_3160 at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.259 kb on + strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.260 kb on - strand at 3333.270 kb on + strand at 3333.270 kb on + strand at 3333.271 kb on - strand at 3333.271 kb on - strand at 3333.345 kb on + strand at 3333.345 kb on + strand at 3333.345 kb on + strand at 3333.345 kb on + strand at 3333.345 kb on + strand at 3333.346 kb on - strand at 3333.346 kb on - strand at 3333.366 kb on + strand at 3333.367 kb on - strand at 3333.367 kb on - strand at 3333.367 kb on - strand at 3333.382 kb on + strand, within Psest_3161 at 3333.383 kb on - strand, within Psest_3161 at 3333.383 kb on - strand, within Psest_3161 at 3333.383 kb on - strand, within Psest_3161 at 3333.540 kb on - strand, within Psest_3161 at 3333.585 kb on + strand, within Psest_3161 at 3333.586 kb on - strand, within Psest_3161 at 3333.586 kb on - strand, within Psest_3161 at 3333.586 kb on - strand, within Psest_3161 at 3333.636 kb on + strand, within Psest_3161 at 3333.636 kb on + strand, within Psest_3161 at 3333.636 kb on + strand, within Psest_3161 at 3333.636 kb on + strand, within Psest_3161 at 3333.637 kb on - strand, within Psest_3161 at 3333.637 kb on - strand, within Psest_3161 at 3333.637 kb on - strand, within Psest_3161 at 3333.637 kb on - strand, within Psest_3161 at 3333.637 kb on - strand, within Psest_3161 at 3333.637 kb on - strand, within Psest_3161 at 3334.104 kb on + strand, within Psest_3161 at 3334.105 kb on - strand, within Psest_3161 at 3334.105 kb on - strand, within Psest_3161 at 3334.242 kb on + strand, within Psest_3161 at 3334.243 kb on - strand, within Psest_3161 at 3334.243 kb on - strand, within Psest_3161 at 3334.243 kb on - strand, within Psest_3161 at 3334.354 kb on + strand at 3334.354 kb on + strand at 3334.354 kb on + strand at 3334.354 kb on + strand at 3334.355 kb on - strand at 3334.360 kb on + strand at 3334.360 kb on + strand at 3334.402 kb on + strand at 3334.402 kb on + strand at 3334.402 kb on + strand at 3334.403 kb on - strand at 3334.403 kb on - strand at 3334.403 kb on - strand at 3334.577 kb on - strand, within Psest_3162 at 3334.577 kb on - strand, within Psest_3162 at 3334.741 kb on + strand, within Psest_3162 at 3334.741 kb on + strand, within Psest_3162 at 3334.741 kb on + strand, within Psest_3162 at 3334.742 kb on - strand, within Psest_3162 at 3334.742 kb on - strand, within Psest_3162 at 3335.061 kb on + strand, within Psest_3162 at 3335.062 kb on - strand, within Psest_3162 at 3335.062 kb on - strand, within Psest_3162 at 3335.062 kb on - strand, within Psest_3162 at 3335.122 kb on + strand, within Psest_3162 at 3335.122 kb on + strand, within Psest_3162 at 3335.123 kb on - strand, within Psest_3162 at 3335.123 kb on - strand, within Psest_3162 at 3335.123 kb on - strand, within Psest_3162 at 3335.172 kb on - strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.173 kb on + strand, within Psest_3162 at 3335.174 kb on - strand, within Psest_3162 at 3335.174 kb on - strand, within Psest_3162 at 3335.174 kb on - strand, within Psest_3162 at 3335.174 kb on - strand, within Psest_3162 at 3335.174 kb on - strand, within Psest_3162 at 3335.174 kb on - strand, within Psest_3162 at 3335.256 kb on + strand, within Psest_3162 at 3335.314 kb on + strand, within Psest_3162 at 3335.314 kb on + strand, within Psest_3162 at 3335.314 kb on + strand, within Psest_3162 at 3335.315 kb on - strand, within Psest_3162 at 3335.315 kb on - strand, within Psest_3162 at 3335.315 kb on - strand, within Psest_3162
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 3,332,269 + -1.5 3,332,406 + -3.9 3,332,406 + -0.3 3,332,406 + -2.1 3,332,406 + -3.8 3,332,406 + -2.6 3,332,406 + -2.2 3,332,407 - +0.1 3,332,407 - -0.2 3,332,407 - +0.9 3,332,407 - +1.5 3,332,407 - -0.9 3,332,407 - +1.5 3,332,407 - +0.3 3,332,407 - -0.4 3,332,407 - +2.4 3,332,407 - +0.2 3,332,407 - -2.1 3,332,407 - -0.2 3,332,411 + -0.1 3,332,411 + -0.6 3,332,686 + Psest_3160 0.25 +0.3 3,333,259 + -0.8 3,333,259 + -0.4 3,333,259 + -0.2 3,333,259 + -1.9 3,333,259 + -0.3 3,333,259 + -0.5 3,333,259 + -0.7 3,333,259 + -1.1 3,333,259 + -3.5 3,333,259 + -1.5 3,333,259 + -1.0 3,333,259 + -2.3 3,333,259 + -0.9 3,333,259 + -1.7 3,333,259 + -1.6 3,333,260 - -0.5 3,333,260 - -0.7 3,333,260 - -0.3 3,333,260 - -2.2 3,333,260 - -0.8 3,333,260 - +0.1 3,333,260 - -2.2 3,333,260 - -3.1 3,333,260 - -1.6 3,333,260 - -0.6 3,333,260 - +0.0 3,333,260 - -0.6 3,333,270 + -1.2 3,333,270 + -0.5 3,333,271 - -2.6 3,333,271 - +0.4 3,333,345 + +0.9 3,333,345 + -1.2 3,333,345 + -0.9 3,333,345 + -0.8 3,333,345 + -0.4 3,333,346 - -2.6 3,333,346 - +0.2 3,333,366 + -2.2 3,333,367 - -1.2 3,333,367 - -1.7 3,333,367 - -1.2 3,333,382 + Psest_3161 0.10 -2.7 3,333,383 - Psest_3161 0.10 +0.0 3,333,383 - Psest_3161 0.10 -1.6 3,333,383 - Psest_3161 0.10 -0.8 3,333,540 - Psest_3161 0.25 -3.1 3,333,585 + Psest_3161 0.29 -1.5 3,333,586 - Psest_3161 0.29 -1.0 3,333,586 - Psest_3161 0.29 -1.3 3,333,586 - Psest_3161 0.29 -1.1 3,333,636 + Psest_3161 0.33 -2.1 3,333,636 + Psest_3161 0.33 -1.1 3,333,636 + Psest_3161 0.33 -2.8 3,333,636 + Psest_3161 0.33 -1.4 3,333,637 - Psest_3161 0.34 +0.2 3,333,637 - Psest_3161 0.34 -2.5 3,333,637 - Psest_3161 0.34 -1.9 3,333,637 - Psest_3161 0.34 -0.6 3,333,637 - Psest_3161 0.34 -2.4 3,333,637 - Psest_3161 0.34 -0.3 3,334,104 + Psest_3161 0.76 -0.0 3,334,105 - Psest_3161 0.76 -0.6 3,334,105 - Psest_3161 0.76 -1.6 3,334,242 + Psest_3161 0.89 -0.8 3,334,243 - Psest_3161 0.89 -1.4 3,334,243 - Psest_3161 0.89 -1.0 3,334,243 - Psest_3161 0.89 -1.6 3,334,354 + -2.2 3,334,354 + -1.9 3,334,354 + -0.5 3,334,354 + -2.1 3,334,355 - -3.8 3,334,360 + -3.2 3,334,360 + -1.5 3,334,402 + -4.2 3,334,402 + +0.6 3,334,402 + -2.0 3,334,403 - -2.9 3,334,403 - -2.4 3,334,403 - -0.6 3,334,577 - Psest_3162 0.17 -3.5 3,334,577 - Psest_3162 0.17 -0.2 3,334,741 + Psest_3162 0.31 -1.9 3,334,741 + Psest_3162 0.31 +0.4 3,334,741 + Psest_3162 0.31 +0.3 3,334,742 - Psest_3162 0.31 -0.6 3,334,742 - Psest_3162 0.31 -2.8 3,335,061 + Psest_3162 0.58 -0.1 3,335,062 - Psest_3162 0.58 -1.6 3,335,062 - Psest_3162 0.58 -0.3 3,335,062 - Psest_3162 0.58 -0.7 3,335,122 + Psest_3162 0.63 +0.1 3,335,122 + Psest_3162 0.63 -0.2 3,335,123 - Psest_3162 0.63 +0.6 3,335,123 - Psest_3162 0.63 +1.2 3,335,123 - Psest_3162 0.63 -2.2 3,335,172 - Psest_3162 0.68 -1.7 3,335,173 + Psest_3162 0.68 -1.8 3,335,173 + Psest_3162 0.68 -1.4 3,335,173 + Psest_3162 0.68 -1.0 3,335,173 + Psest_3162 0.68 +2.0 3,335,173 + Psest_3162 0.68 -3.5 3,335,173 + Psest_3162 0.68 -0.2 3,335,173 + Psest_3162 0.68 -3.4 3,335,174 - Psest_3162 0.68 -1.6 3,335,174 - Psest_3162 0.68 -2.6 3,335,174 - Psest_3162 0.68 -3.6 3,335,174 - Psest_3162 0.68 -0.9 3,335,174 - Psest_3162 0.68 +1.3 3,335,174 - Psest_3162 0.68 -0.7 3,335,256 + Psest_3162 0.75 -2.5 3,335,314 + Psest_3162 0.80 +0.3 3,335,314 + Psest_3162 0.80 -1.4 3,335,314 + Psest_3162 0.80 -0.4 3,335,315 - Psest_3162 0.80 -2.7 3,335,315 - Psest_3162 0.80 -0.9 3,335,315 - Psest_3162 0.80 -2.1
Or see this region's nucleotide sequence