Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_2395 and Psest_2396 are separated by 2 nucleotides Psest_2396 and Psest_2397 overlap by 4 nucleotides Psest_2397 and Psest_2398 overlap by 4 nucleotides Psest_2398 and Psest_2399 overlap by 4 nucleotides
Psest_2395: Psest_2395 - Uncharacterized conserved protein, at 2,548,084 to 2,549,043
_2395
Psest_2396: Psest_2396 - Predicted transcriptional regulators, at 2,549,046 to 2,549,981
_2396
Psest_2397: Psest_2397 - Deoxyribodipyrimidine photolyase, at 2,549,978 to 2,551,408
_2397
Psest_2398: Psest_2398 - SnoaL-like polyketide cyclase., at 2,551,405 to 2,551,833
_2398
Psest_2399: Psest_2399 - Short-chain dehydrogenases of various substrate specificities, at 2,551,830 to 2,552,597
_2399
Position (kb)
2549
2550
2551
2552 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2548.978 kb on + strand at 2548.978 kb on + strand at 2548.978 kb on + strand at 2548.978 kb on + strand at 2548.978 kb on + strand at 2548.996 kb on + strand at 2548.997 kb on - strand at 2548.997 kb on - strand at 2549.206 kb on - strand, within Psest_2396 at 2549.562 kb on + strand, within Psest_2396 at 2549.562 kb on + strand, within Psest_2396 at 2549.562 kb on + strand, within Psest_2396 at 2549.562 kb on + strand, within Psest_2396 at 2549.562 kb on + strand, within Psest_2396 at 2549.562 kb on + strand, within Psest_2396 at 2549.563 kb on - strand, within Psest_2396 at 2549.563 kb on - strand, within Psest_2396 at 2549.563 kb on - strand, within Psest_2396 at 2549.760 kb on + strand, within Psest_2396 at 2549.761 kb on - strand, within Psest_2396 at 2549.861 kb on + strand, within Psest_2396 at 2549.861 kb on + strand, within Psest_2396 at 2550.071 kb on + strand at 2550.071 kb on + strand at 2550.071 kb on + strand at 2550.272 kb on + strand, within Psest_2397 at 2550.272 kb on + strand, within Psest_2397 at 2550.272 kb on + strand, within Psest_2397 at 2550.273 kb on - strand, within Psest_2397 at 2550.273 kb on - strand, within Psest_2397 at 2550.273 kb on - strand, within Psest_2397 at 2550.273 kb on - strand, within Psest_2397 at 2550.423 kb on + strand, within Psest_2397 at 2550.650 kb on + strand, within Psest_2397 at 2550.650 kb on + strand, within Psest_2397 at 2550.651 kb on - strand, within Psest_2397 at 2550.651 kb on - strand, within Psest_2397 at 2550.704 kb on + strand, within Psest_2397 at 2550.800 kb on - strand, within Psest_2397 at 2550.800 kb on - strand, within Psest_2397 at 2550.800 kb on - strand, within Psest_2397 at 2550.833 kb on + strand, within Psest_2397 at 2550.834 kb on - strand, within Psest_2397 at 2550.834 kb on - strand, within Psest_2397 at 2550.834 kb on - strand, within Psest_2397 at 2550.834 kb on - strand, within Psest_2397 at 2550.924 kb on - strand, within Psest_2397 at 2551.483 kb on + strand, within Psest_2398 at 2551.484 kb on - strand, within Psest_2398 at 2551.549 kb on + strand, within Psest_2398 at 2551.550 kb on - strand, within Psest_2398 at 2551.564 kb on + strand, within Psest_2398 at 2551.564 kb on + strand, within Psest_2398 at 2551.564 kb on + strand, within Psest_2398 at 2551.630 kb on + strand, within Psest_2398 at 2551.630 kb on + strand, within Psest_2398 at 2551.750 kb on + strand, within Psest_2398 at 2551.751 kb on - strand, within Psest_2398 at 2551.774 kb on + strand, within Psest_2398 at 2551.775 kb on - strand, within Psest_2398 at 2552.100 kb on + strand, within Psest_2399 at 2552.100 kb on + strand, within Psest_2399 at 2552.100 kb on + strand, within Psest_2399 at 2552.100 kb on + strand, within Psest_2399 at 2552.100 kb on + strand, within Psest_2399 at 2552.100 kb on + strand, within Psest_2399 at 2552.101 kb on - strand, within Psest_2399 at 2552.144 kb on + strand, within Psest_2399 at 2552.212 kb on + strand, within Psest_2399 at 2552.212 kb on + strand, within Psest_2399 at 2552.213 kb on - strand, within Psest_2399 at 2552.213 kb on - strand, within Psest_2399 at 2552.233 kb on + strand, within Psest_2399 at 2552.234 kb on - strand, within Psest_2399 at 2552.277 kb on + strand, within Psest_2399 at 2552.277 kb on + strand, within Psest_2399 at 2552.278 kb on - strand, within Psest_2399 at 2552.353 kb on + strand, within Psest_2399 at 2552.353 kb on + strand, within Psest_2399 at 2552.353 kb on + strand, within Psest_2399 at 2552.354 kb on - strand, within Psest_2399 at 2552.354 kb on - strand, within Psest_2399 at 2552.354 kb on - strand, within Psest_2399 at 2552.354 kb on - strand, within Psest_2399
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 2,548,978 + -0.5 2,548,978 + +1.7 2,548,978 + +2.1 2,548,978 + +0.6 2,548,978 + -0.5 2,548,996 + -0.1 2,548,997 - -2.8 2,548,997 - -1.2 2,549,206 - Psest_2396 0.17 -0.6 2,549,562 + Psest_2396 0.55 -1.8 2,549,562 + Psest_2396 0.55 -0.8 2,549,562 + Psest_2396 0.55 -2.0 2,549,562 + Psest_2396 0.55 +0.6 2,549,562 + Psest_2396 0.55 -1.0 2,549,562 + Psest_2396 0.55 -0.6 2,549,563 - Psest_2396 0.55 -0.4 2,549,563 - Psest_2396 0.55 +0.8 2,549,563 - Psest_2396 0.55 -2.0 2,549,760 + Psest_2396 0.76 +1.1 2,549,761 - Psest_2396 0.76 -0.3 2,549,861 + Psest_2396 0.87 +0.1 2,549,861 + Psest_2396 0.87 +1.1 2,550,071 + -1.4 2,550,071 + -2.8 2,550,071 + -0.8 2,550,272 + Psest_2397 0.21 -0.9 2,550,272 + Psest_2397 0.21 -1.4 2,550,272 + Psest_2397 0.21 -0.8 2,550,273 - Psest_2397 0.21 -2.0 2,550,273 - Psest_2397 0.21 -1.2 2,550,273 - Psest_2397 0.21 -0.9 2,550,273 - Psest_2397 0.21 +0.2 2,550,423 + Psest_2397 0.31 -2.0 2,550,650 + Psest_2397 0.47 -0.4 2,550,650 + Psest_2397 0.47 -2.1 2,550,651 - Psest_2397 0.47 -1.6 2,550,651 - Psest_2397 0.47 -3.0 2,550,704 + Psest_2397 0.51 -0.8 2,550,800 - Psest_2397 0.57 -3.3 2,550,800 - Psest_2397 0.57 -2.5 2,550,800 - Psest_2397 0.57 -1.7 2,550,833 + Psest_2397 0.60 -1.3 2,550,834 - Psest_2397 0.60 +0.9 2,550,834 - Psest_2397 0.60 -0.2 2,550,834 - Psest_2397 0.60 +1.6 2,550,834 - Psest_2397 0.60 +0.8 2,550,924 - Psest_2397 0.66 -1.8 2,551,483 + Psest_2398 0.18 +3.1 2,551,484 - Psest_2398 0.18 -1.0 2,551,549 + Psest_2398 0.34 -2.9 2,551,550 - Psest_2398 0.34 +0.0 2,551,564 + Psest_2398 0.37 -1.2 2,551,564 + Psest_2398 0.37 -0.4 2,551,564 + Psest_2398 0.37 -1.4 2,551,630 + Psest_2398 0.52 -2.6 2,551,630 + Psest_2398 0.52 -1.4 2,551,750 + Psest_2398 0.80 -1.8 2,551,751 - Psest_2398 0.81 -3.0 2,551,774 + Psest_2398 0.86 -0.8 2,551,775 - Psest_2398 0.86 -2.6 2,552,100 + Psest_2399 0.35 +0.7 2,552,100 + Psest_2399 0.35 -1.6 2,552,100 + Psest_2399 0.35 +1.1 2,552,100 + Psest_2399 0.35 +1.1 2,552,100 + Psest_2399 0.35 +0.9 2,552,100 + Psest_2399 0.35 +0.3 2,552,101 - Psest_2399 0.35 +0.3 2,552,144 + Psest_2399 0.41 -2.1 2,552,212 + Psest_2399 0.50 -0.7 2,552,212 + Psest_2399 0.50 -1.9 2,552,213 - Psest_2399 0.50 -0.9 2,552,213 - Psest_2399 0.50 -1.9 2,552,233 + Psest_2399 0.52 -0.6 2,552,234 - Psest_2399 0.53 -0.9 2,552,277 + Psest_2399 0.58 -1.4 2,552,277 + Psest_2399 0.58 +0.2 2,552,278 - Psest_2399 0.58 -1.2 2,552,353 + Psest_2399 0.68 -0.4 2,552,353 + Psest_2399 0.68 +1.1 2,552,353 + Psest_2399 0.68 +1.1 2,552,354 - Psest_2399 0.68 -2.0 2,552,354 - Psest_2399 0.68 +1.0 2,552,354 - Psest_2399 0.68 -3.3 2,552,354 - Psest_2399 0.68 -1.6
Or see this region's nucleotide sequence