Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1719 and Psest_1720 overlap by 8 nucleotides Psest_1720 and Psest_1721 are separated by 19 nucleotides Psest_1721 and Psest_1722 overlap by 11 nucleotides
Psest_1719: Psest_1719 - flagellar basal-body M-ring protein/flagellar hook-basal body protein (fliF), at 1,825,053 to 1,826,837
_1719
Psest_1720: Psest_1720 - flagellar motor switch protein FliG, at 1,826,830 to 1,827,846
_1720
Psest_1721: Psest_1721 - Flagellar biosynthesis/type III secretory pathway protein, at 1,827,866 to 1,828,642
_1721
Psest_1722: Psest_1722 - flagellar protein export ATPase FliI, at 1,828,632 to 1,829,990
_1722
Position (kb)
1826
1827
1828 Strain fitness (log2 ratio)
-1
0
1
2
3
4
5 at 1825.833 kb on + strand, within Psest_1719 at 1826.223 kb on + strand, within Psest_1719 at 1826.224 kb on - strand, within Psest_1719 at 1826.224 kb on - strand, within Psest_1719 at 1826.247 kb on + strand, within Psest_1719 at 1826.247 kb on + strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.248 kb on - strand, within Psest_1719 at 1826.249 kb on + strand, within Psest_1719 at 1826.250 kb on - strand, within Psest_1719 at 1826.250 kb on - strand, within Psest_1719 at 1827.073 kb on + strand, within Psest_1720 at 1827.073 kb on + strand, within Psest_1720 at 1827.073 kb on + strand, within Psest_1720 at 1827.073 kb on + strand, within Psest_1720 at 1827.074 kb on - strand, within Psest_1720 at 1827.136 kb on - strand, within Psest_1720 at 1827.153 kb on + strand, within Psest_1720 at 1827.251 kb on - strand, within Psest_1720 at 1827.425 kb on + strand, within Psest_1720 at 1827.425 kb on + strand, within Psest_1720 at 1827.425 kb on + strand, within Psest_1720 at 1827.426 kb on - strand, within Psest_1720 at 1827.460 kb on + strand, within Psest_1720 at 1827.460 kb on + strand, within Psest_1720 at 1827.460 kb on + strand, within Psest_1720 at 1827.460 kb on + strand, within Psest_1720 at 1827.460 kb on + strand, within Psest_1720 at 1827.460 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.543 kb on + strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.544 kb on - strand, within Psest_1720 at 1827.815 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.844 kb on + strand at 1827.845 kb on - strand at 1827.845 kb on - strand at 1827.852 kb on + strand at 1827.861 kb on + strand at 1828.088 kb on + strand, within Psest_1721 at 1828.088 kb on + strand, within Psest_1721 at 1828.089 kb on - strand, within Psest_1721 at 1828.089 kb on - strand, within Psest_1721 at 1828.140 kb on + strand, within Psest_1721 at 1828.674 kb on + strand at 1828.675 kb on - strand at 1828.809 kb on + strand, within Psest_1722 at 1828.809 kb on + strand, within Psest_1722 at 1828.809 kb on + strand, within Psest_1722 at 1828.809 kb on + strand, within Psest_1722 at 1828.810 kb on - strand, within Psest_1722
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,825,833 + Psest_1719 0.44 +0.9 1,826,223 + Psest_1719 0.66 -1.0 1,826,224 - Psest_1719 0.66 +1.8 1,826,224 - Psest_1719 0.66 +2.6 1,826,247 + Psest_1719 0.67 +1.2 1,826,247 + Psest_1719 0.67 +2.0 1,826,248 - Psest_1719 0.67 +1.4 1,826,248 - Psest_1719 0.67 +1.9 1,826,248 - Psest_1719 0.67 +1.2 1,826,248 - Psest_1719 0.67 +1.4 1,826,248 - Psest_1719 0.67 +3.6 1,826,248 - Psest_1719 0.67 +3.0 1,826,248 - Psest_1719 0.67 +2.0 1,826,249 + Psest_1719 0.67 +2.4 1,826,250 - Psest_1719 0.67 +2.6 1,826,250 - Psest_1719 0.67 +1.8 1,827,073 + Psest_1720 0.24 +2.4 1,827,073 + Psest_1720 0.24 +2.8 1,827,073 + Psest_1720 0.24 +2.7 1,827,073 + Psest_1720 0.24 +3.2 1,827,074 - Psest_1720 0.24 +2.1 1,827,136 - Psest_1720 0.30 +0.7 1,827,153 + Psest_1720 0.32 +0.8 1,827,251 - Psest_1720 0.41 +3.0 1,827,425 + Psest_1720 0.59 +1.8 1,827,425 + Psest_1720 0.59 +1.9 1,827,425 + Psest_1720 0.59 +3.0 1,827,426 - Psest_1720 0.59 +1.5 1,827,460 + Psest_1720 0.62 +2.5 1,827,460 + Psest_1720 0.62 +3.1 1,827,460 + Psest_1720 0.62 +2.4 1,827,460 + Psest_1720 0.62 +1.7 1,827,460 + Psest_1720 0.62 +1.8 1,827,460 + Psest_1720 0.62 +2.2 1,827,543 + Psest_1720 0.70 +0.9 1,827,543 + Psest_1720 0.70 +0.9 1,827,543 + Psest_1720 0.70 +2.0 1,827,543 + Psest_1720 0.70 +1.8 1,827,543 + Psest_1720 0.70 +1.4 1,827,543 + Psest_1720 0.70 +1.7 1,827,543 + Psest_1720 0.70 +2.2 1,827,543 + Psest_1720 0.70 +2.2 1,827,544 - Psest_1720 0.70 +3.2 1,827,544 - Psest_1720 0.70 +2.7 1,827,544 - Psest_1720 0.70 +1.8 1,827,544 - Psest_1720 0.70 +1.2 1,827,544 - Psest_1720 0.70 +2.2 1,827,544 - Psest_1720 0.70 +2.5 1,827,544 - Psest_1720 0.70 +1.9 1,827,544 - Psest_1720 0.70 +2.2 1,827,544 - Psest_1720 0.70 +1.5 1,827,544 - Psest_1720 0.70 +2.1 1,827,544 - Psest_1720 0.70 +2.5 1,827,544 - Psest_1720 0.70 +1.8 1,827,544 - Psest_1720 0.70 +2.9 1,827,544 - Psest_1720 0.70 +2.4 1,827,815 + -1.0 1,827,844 + -1.7 1,827,844 + +0.5 1,827,844 + +0.8 1,827,844 + -1.4 1,827,844 + +0.8 1,827,844 + -0.8 1,827,844 + -0.8 1,827,844 + +5.0 1,827,844 + -0.4 1,827,844 + -1.6 1,827,845 - +1.7 1,827,845 - +1.2 1,827,852 + +0.5 1,827,861 + -0.9 1,828,088 + Psest_1721 0.29 +2.2 1,828,088 + Psest_1721 0.29 +1.6 1,828,089 - Psest_1721 0.29 +1.9 1,828,089 - Psest_1721 0.29 +2.0 1,828,140 + Psest_1721 0.35 +0.0 1,828,674 + +1.1 1,828,675 - +0.4 1,828,809 + Psest_1722 0.13 +1.7 1,828,809 + Psest_1722 0.13 +2.2 1,828,809 + Psest_1722 0.13 +2.8 1,828,809 + Psest_1722 0.13 +1.8 1,828,810 - Psest_1722 0.13 +2.5
Or see this region's nucleotide sequence