Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1603 and Psest_1604 overlap by 1 nucleotides Psest_1604 and Psest_1605 overlap by 4 nucleotides Psest_1605 and Psest_1606 are separated by 81 nucleotides Psest_1606 and Psest_1607 are separated by 147 nucleotides
Psest_1603: Psest_1603 - Thiol:disulfide interchange protein, at 1,706,639 to 1,708,375
_1603
Psest_1604: Psest_1604 - Thiol-disulfide isomerase and thioredoxins, at 1,708,375 to 1,709,190
_1604
Psest_1605: Psest_1605 - Protein-disulfide isomerase, at 1,709,187 to 1,709,954
_1605
Psest_1606: Psest_1606 - hypothetical protein, at 1,710,036 to 1,710,707
_1606
Psest_1607: Psest_1607 - Acetyltransferases, at 1,710,855 to 1,711,322
_1607
Position (kb)
1709
1710 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1708.249 kb on - strand at 1708.561 kb on + strand, within Psest_1604 at 1708.562 kb on - strand, within Psest_1604 at 1708.570 kb on + strand, within Psest_1604 at 1708.570 kb on + strand, within Psest_1604 at 1708.834 kb on + strand, within Psest_1604 at 1709.089 kb on + strand, within Psest_1604 at 1709.089 kb on + strand, within Psest_1604 at 1709.089 kb on + strand, within Psest_1604 at 1709.089 kb on + strand, within Psest_1604 at 1709.211 kb on + strand at 1709.358 kb on + strand, within Psest_1605 at 1709.382 kb on + strand, within Psest_1605 at 1709.382 kb on + strand, within Psest_1605 at 1709.383 kb on - strand, within Psest_1605 at 1709.383 kb on - strand, within Psest_1605 at 1709.383 kb on - strand, within Psest_1605 at 1709.383 kb on - strand, within Psest_1605 at 1709.383 kb on - strand, within Psest_1605 at 1709.476 kb on - strand, within Psest_1605 at 1709.476 kb on - strand, within Psest_1605 at 1709.562 kb on + strand, within Psest_1605 at 1709.562 kb on + strand, within Psest_1605 at 1709.562 kb on + strand, within Psest_1605 at 1709.562 kb on + strand, within Psest_1605 at 1709.562 kb on + strand, within Psest_1605 at 1709.562 kb on + strand, within Psest_1605 at 1709.563 kb on - strand, within Psest_1605 at 1709.563 kb on - strand, within Psest_1605 at 1709.563 kb on - strand, within Psest_1605 at 1709.563 kb on - strand, within Psest_1605 at 1709.872 kb on - strand, within Psest_1605 at 1710.030 kb on + strand at 1710.157 kb on + strand, within Psest_1606 at 1710.157 kb on + strand, within Psest_1606 at 1710.157 kb on + strand, within Psest_1606 at 1710.157 kb on + strand, within Psest_1606 at 1710.158 kb on - strand, within Psest_1606 at 1710.171 kb on - strand, within Psest_1606 at 1710.171 kb on - strand, within Psest_1606 at 1710.175 kb on + strand, within Psest_1606 at 1710.176 kb on - strand, within Psest_1606 at 1710.220 kb on + strand, within Psest_1606 at 1710.220 kb on + strand, within Psest_1606 at 1710.220 kb on + strand, within Psest_1606 at 1710.220 kb on + strand, within Psest_1606 at 1710.221 kb on - strand, within Psest_1606 at 1710.221 kb on - strand, within Psest_1606 at 1710.703 kb on + strand at 1710.703 kb on + strand at 1710.703 kb on + strand at 1710.703 kb on + strand at 1710.703 kb on + strand at 1710.704 kb on - strand at 1710.704 kb on - strand at 1710.715 kb on - strand at 1710.778 kb on + strand at 1710.779 kb on - strand at 1710.779 kb on - strand at 1710.820 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,708,249 - +0.7 1,708,561 + Psest_1604 0.23 -0.4 1,708,562 - Psest_1604 0.23 -1.0 1,708,570 + Psest_1604 0.24 +0.7 1,708,570 + Psest_1604 0.24 -0.4 1,708,834 + Psest_1604 0.56 -2.9 1,709,089 + Psest_1604 0.88 -2.3 1,709,089 + Psest_1604 0.88 -0.5 1,709,089 + Psest_1604 0.88 +0.1 1,709,089 + Psest_1604 0.88 -0.1 1,709,211 + -2.4 1,709,358 + Psest_1605 0.22 -0.8 1,709,382 + Psest_1605 0.25 +1.3 1,709,382 + Psest_1605 0.25 -2.1 1,709,383 - Psest_1605 0.26 -1.6 1,709,383 - Psest_1605 0.26 -1.0 1,709,383 - Psest_1605 0.26 -0.5 1,709,383 - Psest_1605 0.26 -1.9 1,709,383 - Psest_1605 0.26 -2.0 1,709,476 - Psest_1605 0.38 +1.2 1,709,476 - Psest_1605 0.38 -0.6 1,709,562 + Psest_1605 0.49 -0.5 1,709,562 + Psest_1605 0.49 -0.2 1,709,562 + Psest_1605 0.49 -1.1 1,709,562 + Psest_1605 0.49 -0.1 1,709,562 + Psest_1605 0.49 -1.5 1,709,562 + Psest_1605 0.49 +1.1 1,709,563 - Psest_1605 0.49 -0.9 1,709,563 - Psest_1605 0.49 -2.1 1,709,563 - Psest_1605 0.49 -0.9 1,709,563 - Psest_1605 0.49 -1.3 1,709,872 - Psest_1605 0.89 -0.6 1,710,030 + +0.3 1,710,157 + Psest_1606 0.18 -0.4 1,710,157 + Psest_1606 0.18 -0.3 1,710,157 + Psest_1606 0.18 -1.1 1,710,157 + Psest_1606 0.18 +0.6 1,710,158 - Psest_1606 0.18 -0.7 1,710,171 - Psest_1606 0.20 -0.6 1,710,171 - Psest_1606 0.20 +0.5 1,710,175 + Psest_1606 0.21 -1.4 1,710,176 - Psest_1606 0.21 -2.1 1,710,220 + Psest_1606 0.27 -0.4 1,710,220 + Psest_1606 0.27 -0.1 1,710,220 + Psest_1606 0.27 +0.4 1,710,220 + Psest_1606 0.27 -0.5 1,710,221 - Psest_1606 0.28 +0.6 1,710,221 - Psest_1606 0.28 +1.0 1,710,703 + +1.4 1,710,703 + -0.1 1,710,703 + -2.7 1,710,703 + +0.4 1,710,703 + -0.3 1,710,704 - +0.1 1,710,704 - -0.0 1,710,715 - -1.6 1,710,778 + +0.0 1,710,779 - +0.6 1,710,779 - -2.3 1,710,820 - -0.8
Or see this region's nucleotide sequence