Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1577 and Psest_1578 are separated by 45 nucleotides Psest_1578 and Psest_1579 are separated by 241 nucleotides Psest_1579 and Psest_1580 are separated by 82 nucleotides Psest_1580 and Psest_1581 are separated by 15 nucleotides
Psest_1577: Psest_1577 - Membrane glycosyltransferase, at 1,681,308 to 1,683,866
_1577
Psest_1578: Psest_1578 - FKBP-type peptidyl-prolyl cis-trans isomerases 1, at 1,683,912 to 1,684,253
_1578
Psest_1579: Psest_1579 - Pirin-related protein, at 1,684,495 to 1,685,193
_1579
Psest_1580: Psest_1580 - NAD(P)H:quinone oxidoreductase, type IV, at 1,685,276 to 1,685,875
_1580
Psest_1581: Psest_1581 - Acetyltransferases, at 1,685,891 to 1,686,391
_1581
Position (kb)
1684
1685
1686 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1683.523 kb on - strand, within Psest_1577 at 1683.588 kb on + strand, within Psest_1577 at 1683.589 kb on - strand, within Psest_1577 at 1683.589 kb on - strand, within Psest_1577 at 1683.589 kb on - strand, within Psest_1577 at 1683.589 kb on - strand, within Psest_1577 at 1683.655 kb on + strand at 1683.656 kb on - strand at 1683.656 kb on - strand at 1683.656 kb on - strand at 1683.769 kb on - strand at 1683.769 kb on - strand at 1684.267 kb on + strand at 1684.371 kb on - strand at 1684.394 kb on - strand at 1684.513 kb on + strand at 1684.513 kb on + strand at 1684.513 kb on + strand at 1684.514 kb on - strand at 1684.514 kb on - strand at 1684.596 kb on + strand, within Psest_1579 at 1684.596 kb on + strand, within Psest_1579 at 1684.693 kb on + strand, within Psest_1579 at 1684.693 kb on + strand, within Psest_1579 at 1684.693 kb on + strand, within Psest_1579 at 1684.693 kb on + strand, within Psest_1579 at 1684.693 kb on + strand, within Psest_1579 at 1684.693 kb on + strand, within Psest_1579 at 1684.693 kb on + strand, within Psest_1579 at 1684.693 kb on + strand, within Psest_1579 at 1684.693 kb on + strand, within Psest_1579 at 1684.693 kb on + strand, within Psest_1579 at 1684.693 kb on + strand, within Psest_1579 at 1684.693 kb on + strand, within Psest_1579 at 1684.694 kb on - strand, within Psest_1579 at 1684.694 kb on - strand, within Psest_1579 at 1684.694 kb on - strand, within Psest_1579 at 1684.694 kb on - strand, within Psest_1579 at 1684.694 kb on - strand, within Psest_1579 at 1684.694 kb on - strand, within Psest_1579 at 1684.694 kb on - strand, within Psest_1579 at 1684.694 kb on - strand, within Psest_1579 at 1684.694 kb on - strand, within Psest_1579 at 1684.694 kb on - strand, within Psest_1579 at 1684.694 kb on - strand, within Psest_1579 at 1684.694 kb on - strand, within Psest_1579 at 1684.804 kb on + strand, within Psest_1579 at 1684.935 kb on + strand, within Psest_1579 at 1685.027 kb on - strand, within Psest_1579 at 1685.053 kb on + strand, within Psest_1579 at 1685.243 kb on + strand at 1685.243 kb on + strand at 1685.244 kb on - strand at 1685.271 kb on + strand at 1685.271 kb on + strand at 1685.271 kb on + strand at 1685.271 kb on + strand at 1685.272 kb on - strand at 1685.272 kb on - strand at 1685.272 kb on - strand at 1685.297 kb on + strand at 1685.298 kb on - strand at 1685.298 kb on - strand at 1685.309 kb on + strand at 1685.340 kb on + strand, within Psest_1580 at 1685.483 kb on + strand, within Psest_1580 at 1685.483 kb on + strand, within Psest_1580 at 1685.484 kb on - strand, within Psest_1580 at 1685.484 kb on - strand, within Psest_1580 at 1685.484 kb on - strand, within Psest_1580 at 1685.484 kb on - strand, within Psest_1580 at 1685.484 kb on - strand, within Psest_1580 at 1685.882 kb on + strand at 1685.883 kb on - strand at 1685.892 kb on + strand at 1685.892 kb on + strand at 1685.893 kb on - strand at 1685.928 kb on + strand at 1685.929 kb on - strand at 1685.970 kb on + strand, within Psest_1581 at 1685.971 kb on - strand, within Psest_1581 at 1685.971 kb on - strand, within Psest_1581 at 1686.016 kb on - strand, within Psest_1581 at 1686.016 kb on - strand, within Psest_1581 at 1686.016 kb on - strand, within Psest_1581
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,683,523 - Psest_1577 0.87 -2.0 1,683,588 + Psest_1577 0.89 +0.7 1,683,589 - Psest_1577 0.89 -1.1 1,683,589 - Psest_1577 0.89 +0.5 1,683,589 - Psest_1577 0.89 -0.9 1,683,589 - Psest_1577 0.89 +1.0 1,683,655 + +2.5 1,683,656 - +0.2 1,683,656 - -1.2 1,683,656 - +1.5 1,683,769 - -1.1 1,683,769 - -1.5 1,684,267 + -2.1 1,684,371 - -1.9 1,684,394 - -2.6 1,684,513 + +0.0 1,684,513 + +0.7 1,684,513 + -1.7 1,684,514 - -0.3 1,684,514 - +0.3 1,684,596 + Psest_1579 0.14 -0.1 1,684,596 + Psest_1579 0.14 -1.5 1,684,693 + Psest_1579 0.28 -1.0 1,684,693 + Psest_1579 0.28 -1.1 1,684,693 + Psest_1579 0.28 +0.7 1,684,693 + Psest_1579 0.28 -0.8 1,684,693 + Psest_1579 0.28 +0.6 1,684,693 + Psest_1579 0.28 +0.1 1,684,693 + Psest_1579 0.28 -0.1 1,684,693 + Psest_1579 0.28 +0.3 1,684,693 + Psest_1579 0.28 -1.1 1,684,693 + Psest_1579 0.28 -1.7 1,684,693 + Psest_1579 0.28 -1.7 1,684,693 + Psest_1579 0.28 -2.2 1,684,694 - Psest_1579 0.28 -1.1 1,684,694 - Psest_1579 0.28 -0.8 1,684,694 - Psest_1579 0.28 +1.7 1,684,694 - Psest_1579 0.28 -1.7 1,684,694 - Psest_1579 0.28 -0.3 1,684,694 - Psest_1579 0.28 -0.2 1,684,694 - Psest_1579 0.28 +1.5 1,684,694 - Psest_1579 0.28 -0.4 1,684,694 - Psest_1579 0.28 -3.5 1,684,694 - Psest_1579 0.28 -0.0 1,684,694 - Psest_1579 0.28 -1.2 1,684,694 - Psest_1579 0.28 -1.4 1,684,804 + Psest_1579 0.44 -0.9 1,684,935 + Psest_1579 0.63 -1.7 1,685,027 - Psest_1579 0.76 +0.8 1,685,053 + Psest_1579 0.80 -0.3 1,685,243 + -0.4 1,685,243 + -0.0 1,685,244 - +0.5 1,685,271 + -1.5 1,685,271 + +0.2 1,685,271 + -0.5 1,685,271 + -0.3 1,685,272 - -0.1 1,685,272 - +3.5 1,685,272 - -1.1 1,685,297 + -2.1 1,685,298 - +0.4 1,685,298 - -0.6 1,685,309 + -0.1 1,685,340 + Psest_1580 0.11 -2.5 1,685,483 + Psest_1580 0.34 -0.1 1,685,483 + Psest_1580 0.34 -0.6 1,685,484 - Psest_1580 0.35 -2.5 1,685,484 - Psest_1580 0.35 -0.2 1,685,484 - Psest_1580 0.35 -0.8 1,685,484 - Psest_1580 0.35 -1.6 1,685,484 - Psest_1580 0.35 +0.1 1,685,882 + -1.1 1,685,883 - -0.3 1,685,892 + +1.7 1,685,892 + +0.9 1,685,893 - -0.9 1,685,928 + +0.3 1,685,929 - +0.2 1,685,970 + Psest_1581 0.16 +0.7 1,685,971 - Psest_1581 0.16 -0.6 1,685,971 - Psest_1581 0.16 -1.2 1,686,016 - Psest_1581 0.25 -1.2 1,686,016 - Psest_1581 0.25 -0.4 1,686,016 - Psest_1581 0.25 -0.1
Or see this region's nucleotide sequence