Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1447 and Psest_1448 are separated by 75 nucleotides Psest_1448 and Psest_1449 are separated by 80 nucleotides Psest_1449 and Psest_1450 are separated by 8 nucleotides
Psest_1447: Psest_1447 - Putative threonine efflux protein, at 1,556,137 to 1,556,772
_1447
Psest_1448: Psest_1448 - Uncharacterized protein conserved in bacteria, at 1,556,848 to 1,557,303
_1448
Psest_1449: Psest_1449 - Short-chain dehydrogenases of various substrate specificities, at 1,557,384 to 1,558,358
_1449
Psest_1450: Psest_1450 - Transcriptional regulator, at 1,558,367 to 1,559,293
_1450
Position (kb)
1557
1558
1559 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1556.384 kb on - strand, within Psest_1447 at 1556.533 kb on - strand, within Psest_1447 at 1556.602 kb on - strand, within Psest_1447 at 1556.847 kb on + strand at 1556.847 kb on + strand at 1556.847 kb on + strand at 1556.847 kb on + strand at 1556.847 kb on + strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1556.848 kb on - strand at 1557.053 kb on - strand, within Psest_1448 at 1557.105 kb on + strand, within Psest_1448 at 1557.105 kb on + strand, within Psest_1448 at 1557.105 kb on + strand, within Psest_1448 at 1557.106 kb on - strand, within Psest_1448 at 1557.106 kb on - strand, within Psest_1448 at 1557.109 kb on + strand, within Psest_1448 at 1557.109 kb on + strand, within Psest_1448 at 1557.109 kb on + strand, within Psest_1448 at 1557.109 kb on + strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.110 kb on - strand, within Psest_1448 at 1557.197 kb on - strand, within Psest_1448 at 1557.256 kb on + strand, within Psest_1448 at 1557.256 kb on + strand, within Psest_1448 at 1557.256 kb on + strand, within Psest_1448 at 1557.257 kb on - strand, within Psest_1448 at 1557.352 kb on + strand at 1557.352 kb on + strand at 1557.352 kb on + strand at 1557.356 kb on + strand at 1557.356 kb on + strand at 1557.357 kb on - strand at 1557.357 kb on - strand at 1557.357 kb on - strand at 1557.357 kb on - strand at 1557.418 kb on + strand at 1557.745 kb on - strand, within Psest_1449 at 1557.774 kb on + strand, within Psest_1449 at 1557.775 kb on - strand, within Psest_1449 at 1557.932 kb on + strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.933 kb on - strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.990 kb on + strand, within Psest_1449 at 1557.991 kb on - strand at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1557.991 kb on - strand, within Psest_1449 at 1558.082 kb on + strand, within Psest_1449 at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand at 1559.302 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,556,384 - Psest_1447 0.39 -1.7 1,556,533 - Psest_1447 0.62 +0.8 1,556,602 - Psest_1447 0.73 -0.8 1,556,847 + -0.0 1,556,847 + -1.3 1,556,847 + +0.1 1,556,847 + +0.1 1,556,847 + -1.6 1,556,848 - -1.1 1,556,848 - +0.5 1,556,848 - -0.1 1,556,848 - -0.3 1,556,848 - -0.7 1,556,848 - -1.0 1,556,848 - -0.9 1,556,848 - -0.7 1,556,848 - -0.7 1,557,053 - Psest_1448 0.45 -0.7 1,557,105 + Psest_1448 0.56 -1.4 1,557,105 + Psest_1448 0.56 +2.7 1,557,105 + Psest_1448 0.56 +0.7 1,557,106 - Psest_1448 0.57 -1.7 1,557,106 - Psest_1448 0.57 -0.9 1,557,109 + Psest_1448 0.57 -0.3 1,557,109 + Psest_1448 0.57 -2.5 1,557,109 + Psest_1448 0.57 -3.3 1,557,109 + Psest_1448 0.57 -2.0 1,557,110 - Psest_1448 0.57 +1.7 1,557,110 - Psest_1448 0.57 +0.1 1,557,110 - Psest_1448 0.57 -1.5 1,557,110 - Psest_1448 0.57 +1.5 1,557,110 - Psest_1448 0.57 +2.1 1,557,110 - Psest_1448 0.57 +0.1 1,557,110 - Psest_1448 0.57 +1.3 1,557,110 - Psest_1448 0.57 +0.7 1,557,110 - Psest_1448 0.57 +0.3 1,557,110 - Psest_1448 0.57 +0.1 1,557,110 - Psest_1448 0.57 -0.4 1,557,110 - Psest_1448 0.57 -0.3 1,557,110 - Psest_1448 0.57 -1.8 1,557,110 - Psest_1448 0.57 +0.1 1,557,110 - Psest_1448 0.57 -1.6 1,557,110 - Psest_1448 0.57 +0.6 1,557,110 - Psest_1448 0.57 +1.1 1,557,110 - Psest_1448 0.57 -0.3 1,557,110 - Psest_1448 0.57 +0.5 1,557,110 - Psest_1448 0.57 -0.9 1,557,197 - Psest_1448 0.77 +0.2 1,557,256 + Psest_1448 0.89 -1.1 1,557,256 + Psest_1448 0.89 -2.0 1,557,256 + Psest_1448 0.89 +0.7 1,557,257 - Psest_1448 0.90 +0.9 1,557,352 + -1.7 1,557,352 + -0.9 1,557,352 + -0.9 1,557,356 + -0.3 1,557,356 + -2.0 1,557,357 - -1.1 1,557,357 - +0.7 1,557,357 - -0.7 1,557,357 - -0.3 1,557,418 + -1.1 1,557,745 - Psest_1449 0.37 -2.1 1,557,774 + Psest_1449 0.40 +0.3 1,557,775 - Psest_1449 0.40 -2.9 1,557,932 + Psest_1449 0.56 +0.5 1,557,933 - Psest_1449 0.56 -1.1 1,557,933 - Psest_1449 0.56 -0.9 1,557,933 - Psest_1449 0.56 -3.5 1,557,933 - Psest_1449 0.56 +1.3 1,557,990 + Psest_1449 0.62 +0.1 1,557,990 + Psest_1449 0.62 -0.8 1,557,990 + Psest_1449 0.62 -0.3 1,557,990 + Psest_1449 0.62 -2.1 1,557,990 + Psest_1449 0.62 -1.1 1,557,990 + Psest_1449 0.62 +0.2 1,557,990 + Psest_1449 0.62 -1.3 1,557,990 + Psest_1449 0.62 -1.5 1,557,990 + Psest_1449 0.62 -0.8 1,557,991 - +1.7 1,557,991 - Psest_1449 0.62 +0.4 1,557,991 - Psest_1449 0.62 -1.8 1,557,991 - Psest_1449 0.62 -2.0 1,557,991 - Psest_1449 0.62 -2.2 1,557,991 - Psest_1449 0.62 -0.3 1,557,991 - Psest_1449 0.62 -0.6 1,557,991 - Psest_1449 0.62 +0.4 1,557,991 - Psest_1449 0.62 -1.1 1,558,082 + Psest_1449 0.72 -2.1 1,559,302 - +0.9 1,559,302 - -2.0 1,559,302 - +0.1 1,559,302 - +0.1 1,559,302 - -1.8 1,559,302 - -0.4 1,559,302 - -0.9
Or see this region's nucleotide sequence