Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1408 and Psest_1409 are separated by 14 nucleotides Psest_1409 and Psest_1411 are separated by 192 nucleotides Psest_1411 and Psest_1412 are separated by 127 nucleotides
Psest_1408: Psest_1408 - Predicted unusual protein kinase, at 1,516,088 to 1,517,395
_1408
Psest_1409: Psest_1409 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 1,517,410 to 1,518,111
_1409
Psest_1411: Psest_1411 - AraC-type DNA-binding domain-containing proteins, at 1,518,304 to 1,519,047
_1411
Psest_1412: Psest_1412 - Protein of unknown function (DUF3047)., at 1,519,175 to 1,520,332
_1412
Position (kb)
1518
1519
1520 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 1517.354 kb on - strand at 1517.354 kb on - strand at 1517.354 kb on - strand at 1517.393 kb on + strand at 1517.394 kb on - strand at 1517.849 kb on - strand, within Psest_1409 at 1518.079 kb on + strand at 1518.079 kb on + strand at 1518.079 kb on + strand at 1518.079 kb on + strand at 1518.079 kb on + strand at 1518.079 kb on + strand at 1518.080 kb on - strand at 1518.080 kb on - strand at 1518.080 kb on - strand at 1518.085 kb on + strand at 1518.085 kb on + strand at 1518.085 kb on + strand at 1518.085 kb on + strand at 1518.086 kb on - strand at 1518.086 kb on - strand at 1518.102 kb on - strand at 1518.261 kb on + strand at 1518.261 kb on + strand at 1518.359 kb on + strand at 1518.360 kb on - strand at 1518.360 kb on - strand at 1518.360 kb on - strand at 1518.368 kb on + strand at 1518.369 kb on - strand at 1518.369 kb on - strand at 1518.444 kb on + strand, within Psest_1411 at 1518.445 kb on - strand, within Psest_1411 at 1518.445 kb on - strand, within Psest_1411 at 1518.446 kb on + strand, within Psest_1411 at 1518.446 kb on + strand, within Psest_1411 at 1518.446 kb on + strand, within Psest_1411 at 1518.446 kb on + strand, within Psest_1411 at 1518.446 kb on + strand, within Psest_1411 at 1518.446 kb on + strand, within Psest_1411 at 1518.446 kb on + strand, within Psest_1411 at 1518.446 kb on + strand, within Psest_1411 at 1518.446 kb on + strand, within Psest_1411 at 1518.446 kb on + strand, within Psest_1411 at 1518.446 kb on + strand, within Psest_1411 at 1518.447 kb on - strand, within Psest_1411 at 1518.447 kb on - strand, within Psest_1411 at 1518.447 kb on - strand, within Psest_1411 at 1518.447 kb on - strand, within Psest_1411 at 1518.707 kb on + strand, within Psest_1411 at 1518.707 kb on + strand, within Psest_1411 at 1519.004 kb on + strand at 1519.005 kb on - strand at 1519.078 kb on - strand at 1519.104 kb on + strand at 1519.120 kb on + strand at 1519.120 kb on + strand at 1519.120 kb on + strand at 1519.120 kb on + strand at 1519.120 kb on + strand at 1519.120 kb on + strand at 1519.121 kb on - strand at 1519.121 kb on - strand at 1519.121 kb on - strand at 1519.121 kb on - strand at 1519.307 kb on + strand, within Psest_1412 at 1519.307 kb on + strand, within Psest_1412 at 1519.308 kb on - strand, within Psest_1412 at 1519.577 kb on + strand, within Psest_1412 at 1519.738 kb on + strand, within Psest_1412 at 1519.738 kb on + strand, within Psest_1412 at 1519.994 kb on + strand, within Psest_1412 at 1519.994 kb on + strand, within Psest_1412 at 1519.994 kb on + strand, within Psest_1412 at 1520.035 kb on + strand, within Psest_1412 at 1520.036 kb on - strand, within Psest_1412 at 1520.036 kb on - strand, within Psest_1412 at 1520.036 kb on - strand, within Psest_1412 at 1520.036 kb on - strand, within Psest_1412 at 1520.042 kb on + strand, within Psest_1412 at 1520.043 kb on - strand, within Psest_1412 at 1520.043 kb on - strand, within Psest_1412 at 1520.043 kb on - strand, within Psest_1412 at 1520.043 kb on - strand, within Psest_1412 at 1520.043 kb on - strand, within Psest_1412
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,517,354 - -1.2 1,517,354 - +1.3 1,517,354 - +0.5 1,517,393 + -1.4 1,517,394 - -0.2 1,517,849 - Psest_1409 0.63 -1.4 1,518,079 + +3.9 1,518,079 + +0.9 1,518,079 + +0.6 1,518,079 + -0.6 1,518,079 + -1.1 1,518,079 + -0.4 1,518,080 - -0.3 1,518,080 - -0.4 1,518,080 - -0.2 1,518,085 + +0.9 1,518,085 + +0.0 1,518,085 + +0.4 1,518,085 + +1.6 1,518,086 - +0.6 1,518,086 - -1.5 1,518,102 - +1.2 1,518,261 + -0.7 1,518,261 + +0.1 1,518,359 + -0.7 1,518,360 - +0.6 1,518,360 - -2.2 1,518,360 - -0.7 1,518,368 + +1.0 1,518,369 - +0.2 1,518,369 - -1.0 1,518,444 + Psest_1411 0.19 +0.6 1,518,445 - Psest_1411 0.19 -0.5 1,518,445 - Psest_1411 0.19 -0.3 1,518,446 + Psest_1411 0.19 +1.6 1,518,446 + Psest_1411 0.19 -1.7 1,518,446 + Psest_1411 0.19 -1.0 1,518,446 + Psest_1411 0.19 -1.0 1,518,446 + Psest_1411 0.19 +0.6 1,518,446 + Psest_1411 0.19 +0.0 1,518,446 + Psest_1411 0.19 -1.0 1,518,446 + Psest_1411 0.19 -1.4 1,518,446 + Psest_1411 0.19 -1.4 1,518,446 + Psest_1411 0.19 +0.6 1,518,446 + Psest_1411 0.19 -2.0 1,518,447 - Psest_1411 0.19 -1.6 1,518,447 - Psest_1411 0.19 -0.5 1,518,447 - Psest_1411 0.19 +1.0 1,518,447 - Psest_1411 0.19 +0.0 1,518,707 + Psest_1411 0.54 +0.0 1,518,707 + Psest_1411 0.54 -1.4 1,519,004 + -1.2 1,519,005 - +0.5 1,519,078 - -2.7 1,519,104 + +0.1 1,519,120 + -0.4 1,519,120 + +1.6 1,519,120 + +2.2 1,519,120 + +0.6 1,519,120 + -1.0 1,519,120 + -0.6 1,519,121 - +0.3 1,519,121 - -1.3 1,519,121 - -1.6 1,519,121 - -0.4 1,519,307 + Psest_1412 0.11 +0.9 1,519,307 + Psest_1412 0.11 +0.4 1,519,308 - Psest_1412 0.11 +0.1 1,519,577 + Psest_1412 0.35 +0.2 1,519,738 + Psest_1412 0.49 -1.2 1,519,738 + Psest_1412 0.49 +1.0 1,519,994 + Psest_1412 0.71 -1.2 1,519,994 + Psest_1412 0.71 +0.3 1,519,994 + Psest_1412 0.71 +0.2 1,520,035 + Psest_1412 0.74 -1.0 1,520,036 - Psest_1412 0.74 -0.4 1,520,036 - Psest_1412 0.74 +1.6 1,520,036 - Psest_1412 0.74 +0.6 1,520,036 - Psest_1412 0.74 -0.2 1,520,042 + Psest_1412 0.75 -0.8 1,520,043 - Psest_1412 0.75 +2.8 1,520,043 - Psest_1412 0.75 -1.2 1,520,043 - Psest_1412 0.75 +0.0 1,520,043 - Psest_1412 0.75 -1.9 1,520,043 - Psest_1412 0.75 -0.4
Or see this region's nucleotide sequence