Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1404 and Psest_1405 are separated by 180 nucleotides Psest_1405 and Psest_1406 are separated by 9 nucleotides Psest_1406 and Psest_1407 overlap by 17 nucleotides
Psest_1404: Psest_1404 - Methyltransferase domain., at 1,511,192 to 1,511,812
_1404
Psest_1405: Psest_1405 - hypothetical protein, at 1,511,993 to 1,512,430
_1405
Psest_1406: Psest_1406 - His Kinase A (phosphoacceptor) domain./Response regulator receiver domain./Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase., at 1,512,440 to 1,514,143
_1406
Psest_1407: Psest_1407 - RecA-superfamily ATPases implicated in signal transduction, at 1,514,127 to 1,515,653
_1407
Position (kb)
1512
1513
1514
1515 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1511.486 kb on + strand, within Psest_1404 at 1511.486 kb on + strand, within Psest_1404 at 1511.486 kb on + strand, within Psest_1404 at 1511.486 kb on + strand, within Psest_1404 at 1511.487 kb on - strand, within Psest_1404 at 1511.781 kb on + strand at 1511.781 kb on + strand at 1511.781 kb on + strand at 1511.781 kb on + strand at 1511.782 kb on - strand at 1511.782 kb on - strand at 1511.834 kb on + strand at 1511.834 kb on + strand at 1511.834 kb on + strand at 1511.835 kb on - strand at 1511.874 kb on + strand at 1511.978 kb on + strand at 1511.978 kb on + strand at 1511.978 kb on + strand at 1511.979 kb on - strand at 1511.979 kb on - strand at 1511.979 kb on - strand at 1511.979 kb on - strand at 1511.996 kb on + strand at 1511.996 kb on + strand at 1511.997 kb on - strand at 1511.997 kb on - strand at 1511.997 kb on - strand at 1511.997 kb on - strand at 1511.997 kb on - strand at 1511.997 kb on - strand at 1512.000 kb on + strand at 1512.000 kb on + strand at 1512.000 kb on + strand at 1512.000 kb on + strand at 1512.000 kb on + strand at 1512.000 kb on + strand at 1512.001 kb on - strand at 1512.001 kb on - strand at 1512.001 kb on - strand at 1512.013 kb on - strand at 1512.108 kb on + strand, within Psest_1405 at 1512.108 kb on + strand, within Psest_1405 at 1512.108 kb on + strand, within Psest_1405 at 1512.108 kb on + strand, within Psest_1405 at 1512.108 kb on + strand, within Psest_1405 at 1512.108 kb on + strand, within Psest_1405 at 1512.108 kb on + strand, within Psest_1405 at 1512.109 kb on - strand, within Psest_1405 at 1512.109 kb on - strand, within Psest_1405 at 1512.109 kb on - strand, within Psest_1405 at 1512.109 kb on - strand, within Psest_1405 at 1512.109 kb on - strand, within Psest_1405 at 1512.109 kb on - strand, within Psest_1405 at 1512.109 kb on - strand, within Psest_1405 at 1512.109 kb on - strand, within Psest_1405 at 1512.111 kb on + strand, within Psest_1405 at 1512.111 kb on + strand, within Psest_1405 at 1512.111 kb on + strand, within Psest_1405 at 1512.111 kb on + strand, within Psest_1405 at 1512.111 kb on + strand, within Psest_1405 at 1512.111 kb on + strand, within Psest_1405 at 1512.112 kb on - strand, within Psest_1405 at 1512.112 kb on - strand, within Psest_1405 at 1512.112 kb on - strand, within Psest_1405 at 1512.112 kb on - strand, within Psest_1405 at 1512.123 kb on - strand, within Psest_1405 at 1512.289 kb on + strand, within Psest_1405 at 1512.353 kb on + strand, within Psest_1405 at 1512.467 kb on + strand at 1512.467 kb on + strand at 1512.467 kb on + strand at 1512.467 kb on + strand at 1512.467 kb on + strand at 1512.468 kb on - strand at 1512.468 kb on - strand at 1512.468 kb on - strand at 1512.468 kb on - strand at 1512.468 kb on - strand at 1512.468 kb on - strand at 1512.520 kb on - strand at 1512.818 kb on + strand, within Psest_1406 at 1512.819 kb on - strand, within Psest_1406 at 1513.729 kb on - strand, within Psest_1406 at 1513.787 kb on + strand, within Psest_1406 at 1514.006 kb on + strand at 1514.006 kb on + strand at 1514.006 kb on + strand at 1514.006 kb on + strand at 1514.006 kb on + strand at 1514.006 kb on + strand at 1514.006 kb on + strand at 1514.007 kb on - strand at 1514.007 kb on - strand at 1514.108 kb on + strand at 1514.108 kb on + strand at 1514.109 kb on - strand at 1514.179 kb on + strand at 1514.197 kb on - strand at 1514.197 kb on - strand at 1514.247 kb on - strand at 1514.342 kb on - strand, within Psest_1407 at 1514.371 kb on + strand, within Psest_1407 at 1514.371 kb on + strand, within Psest_1407 at 1514.372 kb on - strand, within Psest_1407 at 1514.372 kb on - strand, within Psest_1407 at 1514.372 kb on - strand, within Psest_1407 at 1514.372 kb on - strand, within Psest_1407 at 1514.372 kb on - strand, within Psest_1407 at 1514.372 kb on - strand, within Psest_1407 at 1514.424 kb on + strand, within Psest_1407 at 1514.425 kb on - strand, within Psest_1407 at 1514.425 kb on - strand, within Psest_1407 at 1514.458 kb on + strand, within Psest_1407 at 1514.458 kb on + strand, within Psest_1407 at 1514.458 kb on + strand, within Psest_1407 at 1514.459 kb on - strand, within Psest_1407 at 1514.459 kb on - strand, within Psest_1407 at 1514.519 kb on - strand, within Psest_1407 at 1514.560 kb on - strand, within Psest_1407 at 1514.565 kb on + strand, within Psest_1407 at 1514.764 kb on + strand, within Psest_1407 at 1514.930 kb on - strand, within Psest_1407 at 1514.968 kb on + strand, within Psest_1407 at 1515.031 kb on + strand, within Psest_1407 at 1515.032 kb on - strand, within Psest_1407
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,511,486 + Psest_1404 0.47 -0.9 1,511,486 + Psest_1404 0.47 +1.6 1,511,486 + Psest_1404 0.47 +0.4 1,511,486 + Psest_1404 0.47 -1.4 1,511,487 - Psest_1404 0.48 +0.6 1,511,781 + -0.2 1,511,781 + +0.2 1,511,781 + -0.6 1,511,781 + -0.7 1,511,782 - -0.4 1,511,782 - -1.0 1,511,834 + -0.2 1,511,834 + -0.5 1,511,834 + +1.0 1,511,835 - -0.6 1,511,874 + -2.1 1,511,978 + +0.6 1,511,978 + -0.4 1,511,978 + -1.3 1,511,979 - +0.6 1,511,979 - -0.2 1,511,979 - -1.6 1,511,979 - -0.7 1,511,996 + -1.3 1,511,996 + -1.2 1,511,997 - +0.6 1,511,997 - +0.9 1,511,997 - -1.4 1,511,997 - -0.5 1,511,997 - -0.4 1,511,997 - -1.0 1,512,000 + -2.0 1,512,000 + -1.9 1,512,000 + -2.3 1,512,000 + -2.3 1,512,000 + -2.5 1,512,000 + -1.6 1,512,001 - +1.1 1,512,001 - +0.4 1,512,001 - -0.6 1,512,013 - +0.6 1,512,108 + Psest_1405 0.26 -1.1 1,512,108 + Psest_1405 0.26 +0.9 1,512,108 + Psest_1405 0.26 -1.2 1,512,108 + Psest_1405 0.26 +0.1 1,512,108 + Psest_1405 0.26 -1.7 1,512,108 + Psest_1405 0.26 -1.2 1,512,108 + Psest_1405 0.26 -1.0 1,512,109 - Psest_1405 0.26 -2.0 1,512,109 - Psest_1405 0.26 -3.7 1,512,109 - Psest_1405 0.26 +1.3 1,512,109 - Psest_1405 0.26 +1.2 1,512,109 - Psest_1405 0.26 -1.0 1,512,109 - Psest_1405 0.26 -0.7 1,512,109 - Psest_1405 0.26 -0.7 1,512,109 - Psest_1405 0.26 +0.3 1,512,111 + Psest_1405 0.27 -1.4 1,512,111 + Psest_1405 0.27 -2.8 1,512,111 + Psest_1405 0.27 -0.2 1,512,111 + Psest_1405 0.27 -1.6 1,512,111 + Psest_1405 0.27 +0.9 1,512,111 + Psest_1405 0.27 +0.8 1,512,112 - Psest_1405 0.27 -2.2 1,512,112 - Psest_1405 0.27 +1.9 1,512,112 - Psest_1405 0.27 -1.6 1,512,112 - Psest_1405 0.27 +0.3 1,512,123 - Psest_1405 0.30 -0.7 1,512,289 + Psest_1405 0.68 +1.2 1,512,353 + Psest_1405 0.82 +0.4 1,512,467 + -0.7 1,512,467 + +0.0 1,512,467 + -1.0 1,512,467 + +0.4 1,512,467 + -1.1 1,512,468 - -0.1 1,512,468 - +0.0 1,512,468 - -2.0 1,512,468 - +0.9 1,512,468 - -2.4 1,512,468 - -3.2 1,512,520 - -0.5 1,512,818 + Psest_1406 0.22 -0.9 1,512,819 - Psest_1406 0.22 -2.2 1,513,729 - Psest_1406 0.76 -0.8 1,513,787 + Psest_1406 0.79 -3.0 1,514,006 + +0.9 1,514,006 + +0.7 1,514,006 + -0.9 1,514,006 + -1.6 1,514,006 + +0.0 1,514,006 + -1.4 1,514,006 + -0.7 1,514,007 - -2.5 1,514,007 - -0.1 1,514,108 + -0.4 1,514,108 + -1.4 1,514,109 - -0.1 1,514,179 + -1.1 1,514,197 - -2.1 1,514,197 - -1.7 1,514,247 - +0.1 1,514,342 - Psest_1407 0.14 -0.7 1,514,371 + Psest_1407 0.16 +0.1 1,514,371 + Psest_1407 0.16 -1.7 1,514,372 - Psest_1407 0.16 -1.6 1,514,372 - Psest_1407 0.16 -1.6 1,514,372 - Psest_1407 0.16 -1.7 1,514,372 - Psest_1407 0.16 -0.4 1,514,372 - Psest_1407 0.16 -1.7 1,514,372 - Psest_1407 0.16 -2.6 1,514,424 + Psest_1407 0.19 +0.3 1,514,425 - Psest_1407 0.20 -0.1 1,514,425 - Psest_1407 0.20 -0.6 1,514,458 + Psest_1407 0.22 -1.3 1,514,458 + Psest_1407 0.22 +0.3 1,514,458 + Psest_1407 0.22 +1.8 1,514,459 - Psest_1407 0.22 -1.0 1,514,459 - Psest_1407 0.22 -0.0 1,514,519 - Psest_1407 0.26 +1.3 1,514,560 - Psest_1407 0.28 -2.0 1,514,565 + Psest_1407 0.29 -1.7 1,514,764 + Psest_1407 0.42 -0.4 1,514,930 - Psest_1407 0.53 -0.3 1,514,968 + Psest_1407 0.55 +0.0 1,515,031 + Psest_1407 0.59 -0.2 1,515,032 - Psest_1407 0.59 +0.2
Or see this region's nucleotide sequence