Experiment: L-Isoleucine (N)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psest_1360 and Psest_1361 overlap by 4 nucleotides Psest_1361 and Psest_1362 overlap by 8 nucleotides Psest_1362 and Psest_1363 are separated by 7 nucleotides
Psest_1360: Psest_1360 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,470,176 to 1,471,153
_1360
Psest_1361: Psest_1361 - ABC-type nitrate/sulfonate/bicarbonate transport system, permease component, at 1,471,150 to 1,471,911
_1361
Psest_1362: Psest_1362 - ABC-type nitrate/sulfonate/bicarbonate transport system, ATPase component, at 1,471,904 to 1,472,548
_1362
Psest_1363: Psest_1363 - Predicted membrane protein, at 1,472,556 to 1,473,821
_1363
Position (kb)
1471
1472 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1470.165 kb on - strand at 1470.336 kb on + strand, within Psest_1360 at 1470.336 kb on + strand, within Psest_1360 at 1470.336 kb on + strand, within Psest_1360 at 1470.337 kb on - strand, within Psest_1360 at 1470.337 kb on - strand, within Psest_1360 at 1470.337 kb on - strand, within Psest_1360 at 1470.769 kb on + strand, within Psest_1360 at 1471.474 kb on + strand, within Psest_1361 at 1471.474 kb on + strand, within Psest_1361 at 1471.475 kb on - strand, within Psest_1361 at 1471.475 kb on - strand, within Psest_1361 at 1471.475 kb on - strand, within Psest_1361 at 1471.475 kb on - strand, within Psest_1361 at 1471.475 kb on - strand, within Psest_1361 at 1471.475 kb on - strand, within Psest_1361 at 1471.764 kb on + strand, within Psest_1361 at 1471.764 kb on + strand, within Psest_1361 at 1471.764 kb on + strand, within Psest_1361 at 1471.804 kb on + strand, within Psest_1361 at 1472.635 kb on + strand at 1472.635 kb on + strand at 1472.635 kb on + strand at 1472.636 kb on - strand at 1472.636 kb on - strand at 1472.636 kb on - strand at 1472.636 kb on - strand at 1472.716 kb on + strand, within Psest_1363 at 1472.716 kb on + strand, within Psest_1363 at 1472.716 kb on + strand, within Psest_1363 at 1472.716 kb on + strand, within Psest_1363 at 1472.716 kb on + strand, within Psest_1363 at 1472.717 kb on - strand, within Psest_1363 at 1472.717 kb on - strand, within Psest_1363 at 1472.717 kb on - strand, within Psest_1363
Per-strain Table
Position Strand Gene LocusTag Fraction L-Isoleucine (N) remove 1,470,165 - -0.8 1,470,336 + Psest_1360 0.16 -3.1 1,470,336 + Psest_1360 0.16 -1.0 1,470,336 + Psest_1360 0.16 -2.9 1,470,337 - Psest_1360 0.16 +1.0 1,470,337 - Psest_1360 0.16 -1.3 1,470,337 - Psest_1360 0.16 +2.2 1,470,769 + Psest_1360 0.61 -0.0 1,471,474 + Psest_1361 0.43 -2.0 1,471,474 + Psest_1361 0.43 -1.2 1,471,475 - Psest_1361 0.43 -0.5 1,471,475 - Psest_1361 0.43 -1.0 1,471,475 - Psest_1361 0.43 +0.4 1,471,475 - Psest_1361 0.43 -1.5 1,471,475 - Psest_1361 0.43 -2.2 1,471,475 - Psest_1361 0.43 -0.2 1,471,764 + Psest_1361 0.81 -0.9 1,471,764 + Psest_1361 0.81 -1.9 1,471,764 + Psest_1361 0.81 -0.8 1,471,804 + Psest_1361 0.86 -2.5 1,472,635 + -2.4 1,472,635 + -0.0 1,472,635 + -0.5 1,472,636 - -1.1 1,472,636 - +0.4 1,472,636 - +0.1 1,472,636 - -2.5 1,472,716 + Psest_1363 0.13 -0.5 1,472,716 + Psest_1363 0.13 +0.8 1,472,716 + Psest_1363 0.13 -1.0 1,472,716 + Psest_1363 0.13 -1.3 1,472,716 + Psest_1363 0.13 -1.5 1,472,717 - Psest_1363 0.13 -1.0 1,472,717 - Psest_1363 0.13 -1.6 1,472,717 - Psest_1363 0.13 -0.6
Or see this region's nucleotide sequence